Remediation of soils contaminated with oil with a biological product with immobilization of bacteria by carriers from local soils

Abstract

The article shows the effectiveness of the biological product "SHER" developed by "Scientific Industrial Enterprise" AltaiAgroFarm Ltd "(Kazakhstan) on a carrier in the form of screening from local wastes of limestone-shell rock in the process of cleaning soils from oil with a high paraffin content. The results of NGS-sequencing of the consortium of microorganism as part of the probiotic biological product "SHER" established a microbiome of the following type: at the class level 96.15% of all representatives of the consortium were identified as Bacilli, of which 95.69% of bacteria at the order level were identified as Lactobacillales. A method has been developed for immobilizing bacteria of the biological product "SHER" on a carrier in the form of screening out from local waste of shell limestone. Based on the research results, it was established that the immobilization of bacteria of the biological product "SHER" by the carrier in the form of screening of limestone-shell rock showed a high degree of purification of oil-contaminated soil (88.63%). To study the further activity of bacteria, experiments were carried out on the secondary use of the residual stock solution of the biological product "SHER" used in the process of cleaning oil-contaminated soil. The results of experimental studies using residual mother liquor and immobilization by screenings showed the degree of soil purification (45.7%). X-ray spectral analysis determined the elemental composition of the cleaned soil, revealed the presence of a low content of metals: 1.42% Fe and 0.84% Al. It was found that on a carrier in the form of screening out from local wastes of limestone-shell rock, the effectiveness of the biological product "SHER" increases.

Minimization of the Negative Environmental Impact of Oil Sludge by Using it in the Production of Bitumen

“…Table 4 shows the physico-mechanical characteristics of the obtained samples of modified bitumen with an SBS modifier and oil extracted from oil sludge. The ratio of bitumen:sludge:modifier and bitumen:oil:modifier was chosen depending on the type of modifier and characteristics of bitumen, as well as previously studied works [25].…”

“…The results of the experiment on remediation of soils contaminated with paraffin oil using a biological preparation at room temperature showed that at 21 °C, the destruction of oil in 3 days was 88.63 % [25], therefore, the separation of the oil-water emulsion was carried out after 24 hours of settling.…”

“…It is known that in most cases a neutral environment with a pH between 6 and 9 is ideal for biodegradation [24]. So, for our saline (weakly alkaline) soils, the introduction of a carrier in the form of limestone-shell screening improves the contact of oil-contaminated soil with microorganisms [25]. The bacterial cells of the drug immobilized on the carrier are not exposed to weathering and remain viable in hot climates.…”

“…In [25], a method of bioremediation of oil-contaminated soils with a high paraffin content using the drug 'SHER' with immobilization of local wastes of limestone-shell rock is proposed.…”