Release of monomers from orthodontic adhesives

Bationo, Raoul; Jordana, Fabienne; Boileau, Marie-José; Colat-Parros, Jacques

doi:10.1016/j.ajodo.2016.02.027

Cited by 26 publications

(32 citation statements)

References 48 publications

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“…Regarding BPA, current results match with those of Bationo, Jordana, Boileau, and Colat‐Parros (2016) who detected BPA from Blugloo and not from Transbond XT after 24 hours of immersion in Milli‐Q water.…”

Section: Discussionsupporting

confidence: 88%

Cytotoxicity evaluation of dental and orthodontic light‐cured composite resins

Bationo

Rouamba

Diarra

et al. 2020

Clinical & Exp Dental Res

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Introduction The aim of this study was to determine the cytotoxicity of light‐cured composite resins (Clearfil ES‐2, Clearfil ES Flow, Filtek Supreme XTE, Grengloo, Blugloo, Transbond XT, and Transbond LR) then to assess leachable components in contact with human gingival fibroblasts (GFs) and to quantity detected bisphenol A (BPA). Methods Light‐cured composite resin discs were immersed for 24 hours in gingival fibroblastic medium (n = 3 for each product) and in control medium (n = 2 for each product) contained in plate. Cytotoxicity of the products (n = 95) was determined by the measure of cell viability using MTT assay after reading the optical densities of the plates. The analysis of leachable components was done by gas phase chromatography and mass spectrometry (GC–MS) and detected BPA was quantified. The limit of quantification was 0.01 μg/mL. Statistical analyses were performed by using IBM SPSS Statistics 20 and Kruskal–Wallis and Mann–Whitney U‐tests were applied. Results Cell viabilities were between 85 and 90%. Many chemical compounds including triethylene glycol dimethacrylate (TEGDMA) and BPA were identified. The average concentrations were 0.67 μg/mL ± 0.84 in the control medium and 0.73 μg/mL ± 1.05 in the fibroblastic medium. Filtek Supreme XTE presented the highest concentration of BPA with 2.16 μg/mL ± 0.65 and Clearfil ES Flow presented the lowest with 0.25 μg/mL ± 0.35. No BPA was detected with Transbond XT and Transbond LR. Clearfil ES Flow, Filtek Supreme XTE, Grengloo and Transbond LR presented residual TEGDMA. Conclusions Light‐cured composite resins are slightly cytotoxic opposite GFs and release many components including BPA and TEGDMA. Clinical precautions should be taken to decrease the release of these monomers.

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Section: Discussionsupporting

confidence: 88%

Cytotoxicity evaluation of dental and orthodontic light‐cured composite resins

Bationo

Rouamba

Diarra

et al. 2020

Clinical & Exp Dental Res

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“…Therefore, regarding the cytotoxic/genotoxic potential of a product, not only the manufacturer's list of chemical composition must be taken into account but also the residues derived from the synthesis of the raw materials, which may affect the biocompatibility of the product in a negative manner. Therefore, all chemicals with a potential to exert endocrine activity, cytotoxicity, genotoxicity, and allergic reactions should be listed in the product safety profile [53][54][55][56]. In orthodontic treatment, a major exposition to monomers occurs during the application of brackets at the beginning of multiband therapy.…”

Section: Discussionmentioning

confidence: 99%

Genotoxic and cytotoxic potential of methacrylate-based orthodontic adhesives

Taubmann¹,

Willershausen

Al‐Maawi

et al. 2020

Clin Oral Invest

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Objectives The biocompatibility of methacrylate-based adhesives is a topic that is intensively discussed in dentistry. Since only limited evidence concerning the cyto- and genotoxicity of orthodontic adhesives is available, the aim of this study was to measure the genotoxic potential of seven orthodontic methacrylate-based adhesives. Materials and methods The XTT assay was utilized to determine the cytotoxicity of Assure Plus, Assure Bonding Resin, ExciTE F, OptiBond Solo Plus, Scotchbond Universal Adhesive, Transbond MIP, and Transbond XT after an incubation period of 24 h on human gingival fibroblasts. We also performed the γH2AX assay to explore the genotoxic potential of the adhesives within cytotoxic dose ranges after an incubation period of 6 h. Results The XTT assay showed a concentration-dependent reduction in cell viability. The decrease in cellular viability was in the same dose range most significant for Assure Plus, rendering it the adhesive material with the highest cytotoxicity. Employing the γH2AX assay, a concentration-dependent increase in H2AX phosphorylation was detected, indicating induction of DNA damage. Conclusions For most products, a linear correlation between the material concentration and γH2AX foci was observed. The most severe effect on γH2AX focus induction was found for Transbond MIP, which was the only adhesive in the test group containing the co-initiator diphenyliodonium hexafluorophosphate (DPIHP). Clinical relevance The data indicate that orthodontic adhesives, notably Transbond MIP, bear a genotoxic potential. Since the study was performed with in vitro cultivated cells, a direct translation of the findings to in vivo exposure conditions should be considered with great diligence.

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“…Overall, the achieved sensitivities meet the requirements for the assessment of these contaminants in the fruit drinks, with maximum permitted concentrations as defined by the European Union Commission and Codex Alimentarius. Interestingly, the developed HPLC-UV/FL method exhibited significantly lower LOD values for OTA (0.5 ng mL −1 ) and BPA (1.0 ng mL −1 ) compared to other reported methods using CE-PDA (OTA, 11.2 ng mL −1 ; BPA, 55 µg L −1 ) [31,32], and GC-MS (BPA, 20 µg L −1 ) [33], although the LOD values were comparable to those obtained using HPLC-MS/MS (OTA, 0.5 ng mL −1 ; BPA, 0.15 ng mL −1 ) [34,35].…”

Section: Limits Of Detection and Quantitationmentioning

confidence: 73%

Simultaneous Quantitative Assessment of Ochratoxin A, Patulin, 5-Hydroxymethylfurfural, and Bisphenol A in Fruit Drinks Using HPLC with Diode Array-Fluorimetric Detection

Hassan

Othman

et al. 2020

Foods

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The analysis of regulated contaminants in fruit drinks often requires suitable validated and rapid analytical methods for cost-effective food control, and is of considerable interest among the fruit beverage industry. This study demonstrated a rapid and sensitive high-performance liquid chromatography approach for the simultaneous determination of ochratoxin A (OTA), patulin (PAT), 5-hydroxymethylfurfural (HMF), and bisphenol A (BPA) in various fruit drinks. The separations were achieved using a C18 core-shell column with both photo-diode array and fluorimetric detections connected in series. A gradient system consisting of methanol and 0.1% formic acid at a flow rate of 1.2 mL min−1, thermostated at 35 °C, provided fast elution with run time <9 min. Sample pretreatment was optimised to enable extraction of all analytes from fruit drink matrices. The optimised method was validated. Correlation coefficients of R > 0.99 were achieved with detection limits of 0.5 ng mL−1 (OTA), 1.1 ng mL−1 (PAT), 7.9 ng mL−1 (HMF), and 1.0 ng mL−1 (BPA). Recoveries ranged from 82% to 99%. Good relative standard deviations for intraday retention times (≤3.54%) and peak area (≤3.5%) were achieved. The developed multi-contaminants analysis method was successfully applied to determine OTA, PAT, HMF, and BPA in various fruit drinks.

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Release of monomers from orthodontic adhesives

Cited by 26 publications

References 48 publications

Cytotoxicity evaluation of dental and orthodontic light‐cured composite resins

Cytotoxicity evaluation of dental and orthodontic light‐cured composite resins

Genotoxic and cytotoxic potential of methacrylate-based orthodontic adhesives

Simultaneous Quantitative Assessment of Ochratoxin A, Patulin, 5-Hydroxymethylfurfural, and Bisphenol A in Fruit Drinks Using HPLC with Diode Array-Fluorimetric Detection

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