Release of Histamine from Skin during &lt;i&gt;in vitro&lt;/i&gt; Anaphylaxis

Self Cite

The effect of drugs which increase the cellular concentration of cyclic AMP on IgE-mediated cutaneous hypersensitivity reactions in skin was studied using skin from ovalbumin-sensitised rats. Theophylline, which reduces degradation of cyclic AMP, caused inhibition of antigen-induced histamine release. Synergism was demonstrated when adrenaline in a concentration which was not itself inhibitory caused marked enhancement of theophylline inhibition. The pure β-adrenergic agent isoprenaline produced inhibition in extremely low concentrations. Direct evidence of an inhibitory role for cyclic AMP was obtained using dibutyryl cyclic AMP which produced a dose-related inhibition of histamine release from skin. This evidence should lead to trials of β-adrenergic drugs and xanthines in the treatment of immediate hypersensitivity reactions in human skin.

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inhibition of Cutaneous Hypersensitivity by Cyclic AMP

Yamamoto¹,

Greaves²

1972

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“…As part of an investigation into methods of inhibiting this type of cutaneous allergy, an in vitro system using sensitized skin has been de vised [Greaves et al, 1971] in which anaphylactic histamine release from skin slices is measured. In this paper, we describe some simple characteristics of anaphylactic histamine release from skin of actively sensitized guinea pigs.…”

Section: Introductionmentioning

confidence: 99%

Anaphylactic Release of Histamine from Guinea Pig Skin <i>in vitro</i>

Yeoh¹,

Tay²,

Greaves³

1972

Self Cite

An in vitro system for the study of the mechanism of type 1 anaphylactic reactions is described using skin slices from actively sensitized guinea pigs. Anaphylactic histamine release was found to occur with low concentrations of antigen. Release of histamine was rapid and occurred over a narrow temperature range. This system appears to be a useful model for study of specificity of inhibitors of type 1 anaphylactic reaction.

“…The shaved abdominal skin obtained from sensitized guinea pigs was sliced into 500 ,«m thick slices using a hand micro tome [4], The skin slices were washed three times with Tyrode's solution. 30 slices (ap proximately 500 mg wet weight) were incu bated in 7.0 or 3.5 ml of Tyrode's solution in the presence or absence of egg albumin (10 //g/ml) at 37 °C for 20 min.…”

mentioning

confidence: 99%

“…The residual histamine in the slices after in cubation was extracted by boiling for 10 min in 10 ml of Tyrode's solution. His tamine content in samples was determined by bioassay using an isolated atropinized guinea pig ileum, and the magnitude of his-tamine release was expressed as a percent age [4].…”

mentioning

confidence: 99%

<i>In vitro </i>Release of Eosinophil Chemotactic Factor of Anaphylaxis (ECF-A) from Guinea Pig Skin

Kimura¹,

Yamamoto²,

Yamura³

1979

An in vitro release of the eosinophil chemotactic factor of anaphylaxis (ECF-A) in anaphylactic reactions was studied using skin slices from actively sensitized guinea pigs. ECF-A and histamine were released in vitro from actively sensitized guinea pig skin by antigen challenge. The magnitude of ECF-A release did not always parallel that of anaphylactic histamine release in guinea pig skin, suggesting that there may be no correlation between the amount of ECF-A and that of histamine released from the skin in anaphylactic reactions in guinea pigs.