An in vitro system for the study of the mechanism of type 1 anaphylactic reactions is described using skin slices from actively sensitized guinea pigs. Anaphylactic histamine release was found to occur with low concentrations of antigen. Release of histamine was rapid and occurred over a narrow temperature range. This system appears to be a useful model for study of specificity of inhibitors of type 1 anaphylactic reaction.
The effect of disodium cromoglycate (DCG) on spontaneous and evoked histamine release from guinea pig skin in vitro was studied. DCG in 1–4 mm concentration did not inhibit spontaneous histamine release, release by compound 48/80 or, using skin of actively sensitised guinea pigs, antigen-specific histamine release.
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