Release behavior and signaling effect of vitamin D3 in layered double hydroxides-hydroxyapatite/gelatin bone tissue engineering scaffold: An in vitro evaluation

Fayyazbakhsh, Fateme; Solati‐Hashjin, Mehran; Keshtkar, Abbas Ali; Shokrgozar, Mohammad Ali; Dehghan, Mohammad Mehdi; Larijani, Bagher

doi:10.1016/j.colsurfb.2017.07.004

Cited by 48 publications

(32 citation statements)

References 63 publications

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“…The VD3 was loaded into the gelatin prior to crosslinking, and the amount of VD3 released reached a plateau after 24 h of incubation in PBS. [ 42 ] This result is consistent with a finding that fibrinogen gel loaded with hydrophobic small molecules, similar to VD3, could release its payload within a week. [ 32 ] The system here was primarily designed to deliver VD3 locally.…”

Section: Resultsmentioning

confidence: 99%

A Multi‐Functional Implant Induces Bone Formation in a Diabetic Model

Khorsand

Acri

et al. 2020

Adv Healthcare Materials

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Patients with diabetes mellitus (DM) have defective healing of bone fractures. It was previously shown that nonviral gene delivery of plasmid DNA (pDNA) that independently encodes bone morphogenetic protein‐2 (BMP‐2) and fibroblast growth factor‐2 (FGF‐2), acts synergistically to promote bone regeneration in a DM animal model. Additionally, both insulin (INS) and the hormonally active form of vitamin D3, 1α,25‐dihydroxyvitamin D3 (1α,25(OH)2D3) (VD3) have independently been shown to play key roles in regulating bone fracture healing in DM patients. However, these individual therapies fail to adequately stimulate bone regeneration, illustrating a need for novel treatment of bone fractures in diabetic patients. Here, the ability of local delivery of INS and VD3 along with BMP‐2 and FGF‐2 genes is investigated to promote bone formation ectopically in Type‐2 diabetic rats. A composite consisting of VD3 and INS is developed that contains poly(lactic‐co‐glycolic acid) microparticles (MPs) embedded in a fibrin gel surrounded by a collagen matrix that is permeated with polyethylenimine (PEI)‐(pBMP‐2+pFGF‐2) nanoplexes. Using a submuscular osteoinduction model, it is demonstrated that local delivery of INS, VD3, and PEI‐(pBMP‐2+pFGF‐2) significantly improves bone generation compared to other treatments, thusimplicating this approach as a method to promote bone regeneration in DM patients with bone fractures.

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Section: Resultsmentioning

confidence: 99%

A Multi‐Functional Implant Induces Bone Formation in a Diabetic Model

Khorsand

Acri

et al. 2020

Adv Healthcare Materials

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“…LDHs have been suggested as biomaterials and an expansion on our knowledge about their biological responses is necessary, mainly addressing comparative aspects of both of them and considering the heterogeneity of cells, as fibroblasts. We have argued to evaluate whether two compositions of LDHs (Mg 2 Al‐Cl and Zn 2 Al‐Cl) are able to influence fibroblast performance and so reinforcing them as potential nanomaterials to be further explored in tissue bioengineering.…”

Section: Resultsmentioning

confidence: 99%

“…In this context, advances in physics and chemistry area allowed the application of nanoscaled structures on modifying and functionalizing molecules/materials, ameliorating their performance in medical proposes . It is not new the purpose of layered double hydroxides (LDHs) as biomaterial . Recent studies have shown that LDHs are efficient structures in the transport of drugs and assist cellular processes, such as cell differentiation and viability .…”

Section: Introductionmentioning

confidence: 99%

Layered Double Hydroxides Are Promising Nanomaterials for Tissue Bioengineering Application

et al. 2019

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Layered double hydroxides (LDHs) have emerged as promising nanomaterials for human health and although it has achieved some progress on this matter, their application within bioengineering is not fully addressed. This prompted to subject fibroblasts to two compositions of LDHs (Mg2Al‐Cl and Zn2Al‐Cl), considering an acute response. First, LDH particles are addressed by scanning electron microscopy, and no significant effect of the cell culture medium on the shape of LDHs particles is reported although it seems to adsorb some soluble proteins as proposed by energy‐dispersive X‐ray analysis. These LDHs release magnesium, zinc, and aluminum, but there is no cytotoxic or biocompatibility effects. The data show interference to fibroblast adhesion by driving the reorganization of actin‐based cytoskeleton, preliminarily to cell cycle progression. Additionally, these molecular findings are validated by performing a functional wound‐healing assay, which is accompanied by a dynamic extracellular matrix remodeling in response to the LDHs. Altogether, the results show that LDHs nanomaterials modulate cell adhesion, proliferation, and migration, delineating new advances on the biomaterial field applied in the context of soft tissue bioengineering, which must be explored in health disorders, such as wound healing in burn injuries.

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“…However, these methods had a significant role in the progress of the LTE field. 30,135 There are many methods used for TE approaches, including solvent-casting, [137][138][139][140] freeze-drying, 92,95,138,[141][142][143] electrospinning, [144][145][146][147] phase separation, 103,135 and gas foaming. 77 In this study, we represent some of these techniques that have been utilized in LTE.…”

Section: Extracellular Matrixmentioning

confidence: 99%

Liver Tissue Engineering as an Emerging Alternative for Liver Disease Treatment

Mirdamadi

Kalhori

Zakeri

et al. 2020

Tissue Engineering Part B: Reviews

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Chronic liver diseases affect thousands of lives throughout the world every year. The shortage of liver donors for transplantation has been the main driving force to employ alternative methods such as liver tissue engineering (LTE) in fabricating a three-dimensional transplantable liver tissue or enhancing cell delivery techniques alleviating the need for liver donors. LTE consists of three components, cells, ECM (extracellular matrix), and signaling molecules, which we discuss the first and second. The three most common cell sources used in LTE are human and animal primary hepatocytes, and stem cells for different applications. Two major categories of ECM are used to mimic the microenvironment of these cells, named scaffolds and microbeads. Scaffolds have been made by numerous methods with a wide range of synthetic and natural biomaterials. Cell encapsulation has also been utilized by many polymeric biomaterials. To investigate their functions, many properties have been discussed in the literature, such as biochemical, geometrical, and mechanical properties, in both of these categories. Overall, LTE shows excellent potential in assisting hepatic disorders. However, some challenges exist that prevent the practical use of it clinically, making LTE an ongoing research subject in the scientific society.

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Release behavior and signaling effect of vitamin D3 in layered double hydroxides-hydroxyapatite/gelatin bone tissue engineering scaffold: An in vitro evaluation

Cited by 48 publications

References 63 publications

A Multi‐Functional Implant Induces Bone Formation in a Diabetic Model

A Multi‐Functional Implant Induces Bone Formation in a Diabetic Model

Layered Double Hydroxides Are Promising Nanomaterials for Tissue Bioengineering Application

Liver Tissue Engineering as an Emerging Alternative for Liver Disease Treatment

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