Relationships between pronuclei and nucleoli morphology with the prognosis in intracytoplasmic sperm injection (ICSI) cycles.

Rossi-Ferragut, Lia Mara; Iaconelli, Assumpto; Rocha, C.C.; Medeiros, A.R.C; Aoki, Tsutomu; Borges, Edson

doi:10.1016/s0015-0282(01)02364-0

Cited by 2 publications

(2 citation statements)

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“…A previous study that had included 294 ICSI cycles, with clinical pregnancy at 81% of treatment cycles, have been showed similar results. Furthermore, good pronuclei and nucleoli morphology of embryos are likely to show better embryos on day 3, based on the high pregnancy percentages when at least one S0 embryo was transferred (36). Such data have contributed for inclusion of PN classification in our clinical routine for embryo selection.…”

Section: Discussionmentioning

confidence: 97%

The impact of pronuclear orientation to select chromosomally normal embryos

Borges

Rossi

Farah

et al. 2005

J Assist Reprod Genet

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Purpose : To find an effective method to select embryos from ICSI with better chromosomal status when preimplantation-genetic-diagnosis (PGD) is not applied. Methods : Several morpholological evaluations were done in same embryos. Embryos from ICSI were classified on day 1 according to pronuclear-nucleoli arrangement. On day 3, classification was done according to number, fragmentation, size and shape of cells. In some patients, embryos exhibiting good quality on day 3 (at least six regular blastomeres with cell fragmentation lower than 20%) were also submitted to PGD, irrespective to pronuclearnucleoli morphology. Results : Forty-two per cent of normally fertilized embryos showed pronuclear-nucleoli-goodmorphology; from those, 86% were also classified as good quality on day 3. Good-quality embryos submitted to PGD have shown lower chromosomal abnormality rates when also classified as pronuclear-nucleoli-good-morphology. Conclusions : Pronuclear-nucleoli morphology seems to be correlated with PGD results. This criterion may prove useful for pre-select embryos with normal chromosomal package when PGD is not applied.

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Section: Discussionmentioning

confidence: 97%

The impact of pronuclear orientation to select chromosomally normal embryos

Borges

Rossi

Farah

et al. 2005

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…Spermatozoa retrieved from men with severe male factor may show some abnormalities in terms of MTOC or centrioles, on the basis of the fact that zygote was an abnormal morphology (32,33), rarely progress well, and rarely form blastocysts (9). Despite this, in our previous work (34,35) we compared PN morphology, transfer day morphology, and PGD results for chromosomes X, Y, 13, 18, and 21. Our results have showed that preembryos with close or aligned PN exhibit higher percentage of normal PGD results for the analyzed chromosomes (56%) compared to preembryos with unequal or separated PN (45.5%).…”

Section: Discussionmentioning

confidence: 99%