The impact of pronuclear orientation to select chromosomally normal embryos

Borges, Edson; Rossi, Lia Mara; Farah, L; Guilherme, Patrı́cia; Rocha, C.C.; Ortiz, Valdemar; Iaconelli, Assumpto

doi:10.1007/s10815-005-4874-x

Cited by 7 publications

(4 citation statements)

References 48 publications

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“…No relationship between female or male PN and blastocyst formation was found, revealing that protein and energy storage could be more important to the developmental viability of embryos than chromosomal normality, at least if chromosomal errors are not too big. [36][37][38][39][40] The lack of NPB assessment in our model was the main drawback in predicting the occurrence of aneuploidy. From A) The rate of chromosome-normal blastocysts by the ranking of female PN coefficient 𝛽1.…”

Section: Discussionmentioning

confidence: 99%

Variation of Female Pronucleus Reveals Oocyte or Embryo Chromosomal Copy Number Variations

Yang

Wang

et al. 2022

Advanced Genetics

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The characteristics of the human pronuclei (PNs), which exist 16–22 h after fertilization, appear to serve as good indicators to evaluate the quality of human oocyte and embryo, and may reflect the status of female and male chromosome composition. Here, a quantitative PN measurement method that is generated by applying expert experience combined with deep learning from large annotated datasets is reported. After mathematic reconstruction of PNs, significant differences are obtained in chromosome‐normal rate and chromosomal small errors such as copy number variants by comparing the size of the reconstructive female PN. After integrating the whole procedure of PN dynamics and adjusting for errors that occur during PN identification, the results are robust. Notably, all positive prediction results are obtained from the female propositus population. Thus, the size of female PNs may mirror the internal quality of the chromosomal integrity of the oocyte. Embryos that develop from zygotes with larger female PNs may have a reduced risk of copy number variations.

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Section: Discussionmentioning

confidence: 99%

Variation of Female Pronucleus Reveals Oocyte or Embryo Chromosomal Copy Number Variations

Yang

Wang

et al. 2022

Advanced Genetics

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“…A large PN was regarded as an abnormality, and the number of cells with a large PN increased with the duration of 6-DMAP treatment. In human assisted reproductive technology, PN morphology is a key evaluation criterion for fertilized embryo development and screening of chromosome abnormalities (Borges et al, 2005;Scott et al, 2000), and is used to select embryos of good quality and development potential. In the present study, abnormal PNs had a larger diameter than normal PNs and the nucleoli were dispersed within them.…”

Section: Discussionmentioning

confidence: 99%

Successful reprogramming of differentiated cells by somatic cell nuclear transfer, usingin vitro-matured oocytes with a modified activation method

Yan

Zhang

et al. 2012

J Tissue Eng Regen Med

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Therapeutic cloning has tremendous potential for cell therapy and tissue repair in some diseases. However, the efficiency of development of cloned human embryos by somatic cell nuclear transfer is still low. In the present study, the activation of cloned human embryos was investigated while using in vitro-matured oocytes. Pseudo-pronuclear formation and the subsequent development was compared with different activation parameters, including different durations of ionomycin and 6-dimethylaminopurine treatment. The results showed that somatic cells were successfully reprogrammed by modification of activation treatments while using in vitro-matured oocytes. The activation efficiency of cloned human embryos was significantly increased at durations of ionomycin at both 5 and 7 min, despite different durations of 6-DMAP treatment. The results of blastocyst development showed that 20% of activated embryos developed to the blastocyst stage when the embryos were activated with 5 µm ionomycin for 5 min and 2 mm 6-DMAP for 5 h, which was significantly higher than those activated with other parameters. Moreover, we found that an increasing duration of 6-DMAP induced the formation of a single, large, pseudo-pronucleus in cloned human embryos and impaired subsequent development competence. In conclusion, successful reprogramming of human somatic cells was achieved using in vitro-matured oocytes by somatic cell nuclear transfer and improved with a modified activation method.

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“…A morfologia alterada na fase de pronucleos parece também estar diretamente relacionada com maior índice de anormalidades genéticas embrionárias. Estudos 27,28 correlacionam as características do zigoto com os resultados de diagnóstico genético pré-implantacional, demonstrando forte correlação entre os parâmetros analisados. Donadio 25 constatou uma correlação imperfeita, sugerindo perda da acuidade nos critérios pronucleares isolados.…”

Section: Discussionunclassified

Resultados obtidos em mulheres portadoras de endometriose pélvica avançada e mulheres com ligadura tubária, submetidas a ICSI: análise comparativa

Aoki¹,

Donadio²,

Auge³

et al. 2005

Rev. Bras. Ginecol. Obstet.

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Objetivo: análise comparativa dos resultados obtidos em mulheres portadoras de endometriose pélvica avançada e mulheres com ligadura tubária, submetidas a injeção intracitoplasmática de espermatozóide (ICSI). Métodos: noventa e três mulheres com diagnóstico de infertilidade, com ciclo menstrual normal, não submetidas a qualquer tratamento hormonal e cirúrgico durante o último ano, índice de massa corporal igual a 20-25, portadoras de ovários sem a presença de cistos ou tumores participaram deste estudo e foram divididas em dois grupos: tubário (TUB), 39 mulheres com ligadura tubária, e endometriose (EDT), 54 mulheres com EDT-III e EDT-IV, submetidas a indução ovulatória com FSH-r e ICSI. Os dados clínicos e laboratoriais foram comparados. Testes χ 2 , Fisher, t pareado, t de Student e Mann-Whitney foram empregados. Resultados: níveis mais baixos de estradiol (2243,1 vs 1666,3; p=0,001) e número menor de folículos por paciente (16,9 vs 13,9; p=0,001) foram recuperados no grupo EDT, apesar da utilização de maior número de unidades de FSH-r (1775,6 vs 1998,6; p=0,007, para TUB e EDT, respectivamente). Não houve diferença quanto à taxa de recuperação ovocitária (69 vs 73,5%; p=0,071) assim como nas taxas de fertilização normal (83,7 vs 81,7%; p=0,563 respectivamente para TUB e EDT. No entanto, menor número de pré-embriões ótimos foi obtido no grupo EDT (36,4 vs 24,8%, respectivamente para TUB e EDT; p=0,005). As taxas de gestação total (41,0 vs 42,6%; p=0,950) e de implantação (13,9% x 14,5%; p=0,905) não foram significativamente diferentes quando TUB e EDT foram comparados. Conclusões: os ovários das mulheres do grupo EDT parecem ser menos responsivos à indução da ovulação com FSH-r. A endometriose parece comprometer o número médio de folículos e de pré-embriões ótimos sem prejudicar as taxas de recuperação ovocitária e fertilização. No entanto, uma vez formados, os pré-embriões do grupo EDT exibem chances de implantação e gestação semelhantes àquelas do grupo TUB. PALAVRAS-CHAVE:Endometriose/terapia; Infertilidade feminina; Fertilização in vitro; Injeção de esperma intracitoplasmáticas; Medicina reprodutiva ABSTRACT Purpose: comparative analysis of the outcome of women with advanced pelvic endometriosis and women with tubal sterilization submitted to intracytoplasmic sperm injection (ICSI). Methods: ninety-three infertile women, with normal menstrual cycle, without hormonal or surgical treatment during 12 months, body mass index of 20-25, ovaries with no tumors or cysts were included in the present study and divided into two groups: tubal sterilization (TUB), 39 women, and endometriosis (EDT), 54 women with III-EDT and IV-EDT, undergoing ovulatory induction using r-FSH and ICSI. Clinical and laboratorial data were compared. χ 2 , Fisher, Student's t, and Mann-Whitney tests were employed. Results: lower estradiol levels (2,243.1 vs 1,666.3; p=0.001) and lower number of follicles per patient (16.9 vs 13.9, p=0.001) were noted in EDT group, in spite of more units of r-FSH (1,775.6 vs 1,998.6; p=0.007, for TUB and EDT...

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The impact of pronuclear orientation to select chromosomally normal embryos

Cited by 7 publications

References 48 publications

Variation of Female Pronucleus Reveals Oocyte or Embryo Chromosomal Copy Number Variations

Variation of Female Pronucleus Reveals Oocyte or Embryo Chromosomal Copy Number Variations

Successful reprogramming of differentiated cells by somatic cell nuclear transfer, usingin vitro-matured oocytes with a modified activation method

Resultados obtidos em mulheres portadoras de endometriose pélvica avançada e mulheres com ligadura tubária, submetidas a ICSI: análise comparativa

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