Relationship of Serum Interleukin-10 and Its Genetic Variations with Ischemic Stroke in a Chinese General Population

Abstract

Background and PurposeAnti-inflammatory cytokine and its genetic variations may play an important role in the process of atherosclerosis. We assessed whether serum interleukin-10 (IL-10) and its genetic variations are associated with ischemic stroke in a Chinese general population.MethodsAn epidemiological survey on cardiovascular diseases and their risk factors was carried in a general population in Beijing in 2005. Serum IL-10, IL-6, p-selectin, soluble intercellular adhesion molecule-1 and C-reactive protein were analyzed using ELISA kits, while three IL-10 Single Nucleotide Polymorphisms (SNP) (rs1800872, rs1554286 and rs3021094) were genotyped in 1475 participants.ResultsA high serum IL-10 (top tertile) was significantly associated with ischemic stroke (multivariable adjusted odds ratio (OR) =0.50; 95%CI 0.31-0.81). Rs1800872 (AA vs. AC+CC genotype, OR=1.60; 1.06-2.39), rs1554286(TT vs. CT+CC genotype, OR=1.59; 1.06-2.39), and rs3021094 (CC/CA vs. AA genotype, OR=1.64; 1.04-2.60) were all significantly associated with ischemic stroke even after controlling for age, sex, smoking, systolic blood pressure, total cholesterol, glucose, body mass index and serum IL-10. The SNP score (a summary index of these SNPs) and IL-10 (top tertile) together significantly improved the discriminative power in predicting ischemic stroke by 3.3% (95%CI: 0.2-6.4, p=0.0398) compared to predictions based on conventional risk factors alone.ConclusionsThe lower serum IL-10 concentration and its selected genetic variations were significantly associated with an increased likelihood of ischemic stroke in this cross-sectional study. Our results suggest that more prospective studies should be conducted to provide stronger evidence justifying the use of IL-10 and its SNPs as new biomarkers to identify a predisposition towards ischemic stroke.

Interleukin-10 family cytokines pathway: genetic variants and psoriasis

“…analysed the SNP rs1554286 of IL10 in 807 Indian patients and found a strong association between rs1554286 and leprosy . The SNP rs1554286 (TT vs. CT and CC genotype, OR = 1·59; 95% CI 1·06–2·39) was significantly associated with ischaemic stroke even after controlling for age, sex, smoking, systolic blood pressure, total cholesterol, glucose, body mass index and serum IL‐10 in a case–control study …”

Association of the interleukin-10 gene -1082A/G genetic polymorphism with risk of ischemic stroke in a Chinese population

“…The authors of previous studies have reported an association between IL10 gene polymorphisms and the development of cerebrovascular diseases; however, the results are inconsistent (Balding et al, 2004;Trompet et al, 2007;Munshi et al, 2010;Sultana et al, 2011;Xie et al, 2013;Chao et al, 2014). Balding et al (2004) found that IL10 gene polymorphisms were not associated with ischemic stroke.…”

“…Munshi et al (2010) conducted a study in Caucasians and revealed that individuals bearing A allele of IL10 -1082A/G gene were more predisposed to stroke. Xie et al (2013) reported that the individuals carring IL10 rs1800872 and rs3021094 gene variant exhibited a significantly association with risk of ischemic stroke in a Chinese population. A recent meta-analysis pooled 16 studies and revealed that the AA genotype of IL10 -1082A/G is associated with the risk of ischemic stroke (Chao et al, 2014).…”

“…The gene encoding IL-10 (IL10) is present on the chromosomal locus 1q31-32, and several genome regions of chromosome 1 (1q21-23, 1q31-32 and 1q42-44) have been observed to be correlated with development of several kinds of diseases, such as diabetes mellitus, peptic ulcer disease, coronary artery disease, leprosy, chronic hepatitis B infection, and oral cancer (Alvarado-Arnez et al, 2015;Ghaleh Baghi et al, 2015;Hsu et al, 2015;Khodaeian et al, 2015;Miftahussurur and Yamaoka, 2015;Yang et al, 2015). The authors of previous researches have reported the correlation of IL10 gene polymorphisms with the ischemic stroke risk; however, the results are inconsistent (Munshi et al, 2010;Sultana et al, 2011;Xie et al, 2013;Qi et al, 2014). In the present study, we conducted a case-control study to assess the role of two SNPs of IL10 (IL10 -1082A/G and -592C/A) in the ischemic stroke risk.…”

Anti-inflammatory mediators for molecular imaging of atherosclerosis

“…Apart from this, Pinderski Oslund et al found that activated T-lymphocytes overexpress the antiinflammatory cytokine interleukin-10 (IL-10), and that this event is capable of blocking AS actions in vitro and in vivo (57,58). Xie et al showed in a cross-sectional study that decreased serum IL-10 concentrations were significantly associated with an increased likelihood of ischemic stroke (59). Further, IL-10 expression was found elevated in advanced and unstable AS-plaques (60), and it appears to contribute to the regulation of the local inflammatory response, and is involved in the control of cell death within plaques (60)(61)(62)(63).…”