Relationship between TA01 and TA02 polypeptides associated with lung adenocarcinoma and histocytological features

Abstract

Summary TA01 (molecular weight 35.0 kDa, isoelectric point 5.45) and TA02 (molecular weight 35.0 kDa, isoelectric point 5.29) polypeptides were detected using two-dimensional polyacrylamide gel electrophoresis (2-DE). A previous study has shown that these polypeptides are distributed in primary adenocarcinomas and some large -cell carcinomas of the lung. However, various expression levels of TA01 and TA02 polypeptides were demonstrated in adenocarcinoma, while large-cell carcinoma expressed low levels. To evaluate the relationship between the expression of TA01 and TA02 polypeptides and the histocytological features of primary adenocarcinoma of the lung, these two polypeptides were analysed by 2-DE combined with a non-enzymatic sample preparation technique, and their expression levels were compared with the histocytological features of primary lung adenocarcinoma. Out of 57 primary lung adenocarcinoma cases, 46 cases (80.7%) and 52 cases (91.2%) expressed TA01 and TA02 polypeptides respectively. Furthermore, the expression levels of TA01 and TA02 polypeptides correlated with the degree of cellular atypia, structural atypia and histocytological differentiation of primary lung adenocarcinoma. On the other hand, these two polypeptides were not detected in adenocarcinoma of the lung, metastatic from the colon and mammary glands. High expression of TA01 and TA02 polypeptides reflected the differentiation of primary adenocarcinoma in the lung. These two polypeptides are valuable in determining the histocytological differentiation of primary lung adenocarcinoma as well as in distinguishing between primary and metastatic adenocarcinoma of the lung.Keywords: primary adenocarcinoma of the lung; histopathological differentiation; TA01 polypeptide; TA02 polypeptide; two-dimensional polyacrylamide gel electrophoresis Tumour markers can contribute to clinicopathological diagnosis, more accurate evaluation of biological malignancy of the tumour and selection of therapeutic strategy. Products of oncogenes and mutant tumour-suppressor genes are possible candidates for biological tumour markers. Also, histological differentiation antigens could be useful markers in cases of borderline histology.Although some tumour markers (e.g. CEA, CA 19-9 and TPA, etc.) are used in the clinical diagnosis of primary adenocarcinoma of the lung (Vincent et al, 1979;Latanche et al, 1985;Buccheri et al, 1988;Niklinski et al, 1995), there is no specific tumour marker for primary adenocarcinoma of the lung. At present, cell proliferation and products of oncogenes and tumour-suppressor genes in lung adenocarcinoma are attracting attention (M0rkve et al, 1992;Scagliotti et al, 1993;Ebina et al, 1994;Rachwal et al, 1995;Costa et al, 1996) Correspondence to: T Hirano Linnoila et al, 1992) and protein 1 (Barnard et al, 1992). There is a possibility that these polypeptides become specific tumour markers for primary adenocarcinoma of the lung. Such a marker might be useful to distinguish primary and metastatic adenocarcinoma of the lung.We also investigate...

Aspartic proteinase napsin is a useful marker for diagnosis of primary lung adenocarcinoma

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