The aim of this work was to investigate the barrier and comfort properties of protective uncoated and coated-impregnated three-layered knitted fabrics with different arrangements of special yarns, such as conductive yarns and yarns with different filament cross sections. Depending on content (7.5-30%) of conductive PES yarns with carbon core filaments and PES/stainless steel spun yarns, fabrics were grouped into A and B. In order to achieve multifunctional barrier and comfort properties, high porosity polyurethane and fluorocarbon resin coatings were applied. At the beginning of the research, the fabrics of groups A and B were coated with commercially available micro-porous polyurethane foam Tubicoat Õ MB according to the crushed foams method and impregnated with fluorocarbon water-repellent agent Tubiguard Õ 270. The achieved functional, barrier (water and oil repellency, resistance to water penetration, and electrostatic shielding), and comfort (air permeability, water vapor permeability, water retentivity, and drying intensiveness) properties were determined.