Relationship between maternal concentrations of caprine pregnancy-associated glycoprotein in Alpine goats and the number of fetuses using a homologous radioimmunoassay

Batalha, E. S.; Sulon, Joseph; Figueiredo, José Ricardo de; Beckers, Jean‐François; Martins, Gabrimar Araújo; Silva, L. D. M.

doi:10.1016/s0921-4488(01)00242-5

Cited by 8 publications

(6 citation statements)

References 12 publications

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“…This is the first report to describe PAG profile during pregnancy in local Moroccan goats with a high frequency of blood samples during early and late pregnancy and with the aim to separate goats bearing singles from those bearing multiples. The PAG concentrations detected here by a semi‐heterologous RIA are higher than with those previously reported using homologous RIA systems based on antisera against caprine PAG (Batalha et al. 2001b; Gonzalez et al.…”

Section: Discussioncontrasting

confidence: 75%

“…(1999) at the15th, 16th and 17th week of gestation in Moxoto goats. Batalha et al. (2001b) demonstrates the influence of the number of foetuses on PAG levels from about the day 63 of pregnancy until parturition.…”

Section: Discussionmentioning

confidence: 99%

“…1999; Gonzalez et al. 2000; Batalha et al. 2001b) but strong individual variations made these differences sometimes non‐significant.…”

Section: Introductionmentioning

confidence: 99%

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Pregnancy‐Associated Glycoprotein Secretion in North Moroccan Goats

Chentouf

Amiri

Sulon

et al. 2008

Reprod Domestic Animals

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The plasma profile of pregnancy-associated glycoprotein (PAG) and its relationship with fetal number were studied in 14 North Moroccan goats using a semi-heterologous radioimmunoassay (RIA). Peripheral blood was collected every day during the first month of pregnancy, afterward the blood samples were collected trice a week. The PAG were first detected at day 20 of pregnancy, their levels increase to week 8 of gestation then decrease slightly until parturition. Statistical differences between goats carrying one or two foetuses are observed from week 5 of pregnancy until parturition. Factorial Discriminant Analysis provides mathematical models for the discrimination between the litter size using the PAG level and the week of pregnancy. Using only one blood sample per week, high sensitivity, specificity and overall accuracy (74%, 88% and 81%) were obtained using these models. It is concluded that the PAG RIA is an effective tool for early diagnosis of pregnancy and for discrimination between the litter sizes in North Moroccan goats.

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Section: Discussioncontrasting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

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Pregnancy‐Associated Glycoprotein Secretion in North Moroccan Goats

Chentouf

Amiri

Sulon

et al. 2008

Reprod Domestic Animals

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“…Moreover, heterologous PAG tests were also useful for pregnancy diagnoses in wild ruminants, i.e. wood bison [107], moose [108], rocky mountain elk [109], white-tailed deer [110,111], mule deer [111], red deer [112], sika deer [113], fallow deer [114,115], mountain goat [116] and Alpine goats [117][118][119]. The measurement of PAG protein concentrations in maternal blood or milk of ruminants allow for 76.6-100% accuracy rates for early pregnancy diagnoses [120,121] and provide an alternative method to ultrasonography for determining late embryonic mortality [96,122].…”

Section: Pregnancy Diagnostic Pag Testsmentioning

confidence: 99%

“…The concentration of circulating PAGs depends on the number of well-being embryos/foetuses, and is higher in females with multiple than single pregnancies, according to the greater mass of the chorionic membranes in animals bearing twins [4, 70,98,99,117,118]. The secretion of PAGs can also differ based on the sex and breed of the foetus.…”

Section: Pregnancy Diagnostic Pag Testsmentioning

confidence: 99%

Biodiversity of multiple Pregnancy-Associated Glycoprotein (PAG) family: gene cloning and chorionic protein purification in domestic and wild eutherians (Placentalia) – a review

2006

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-This review presents a broad overview of chorionic glycoproteins encoded by the Pregnancy-Associated Glycoprotein (PAG) gene family and also serves to illustrate how the recent discovery of the PAG family has contributed to our general knowledge of genome evolution, placental transcription and placental protein expression. The complex and large PAG family is restricted to the Artiodactyla order, although single PAG-like genes have also been identified in species outside the Artiodactyla. The PAGs are members of the aspartic proteinase (AP) superfamily. Unexpectedly, however, some members of the PAG family possess amino acid substitutions within and around the active site that likely render them unable to act as proteinases. This paper summarises the available information regarding biodiversity of PAG gene expression based on cDNA cloning, mRNA localisation studies and the structural organisation of the PAG genes with a particular emphasis on PAG promoters. It also compares available data regarding PAG protein purifications, sequencing and their N-glycodiversity. Finally, it discusses the scientific relevance, possible functional roles of the PAGs and describes possible profitable applications related to the detection of PAG proteins in the blood of pregnant domestic and wild species. chorionic glycoproteins / eutherian PAG family / diagnostic PAG markers

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