Relationship between Dietary Intake and Tissue Levels of Reduced and Total Vitamin C in the Nonscorbutic Guinea Pig

Berger, Joseph J.; Shepard, Douglas; Morrow, Frank D.; Taylor, Allen

doi:10.1093/jn/119.5.734

Cited by 84 publications

(27 citation statements)

References 25 publications

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“…0.5-inch needle syringe. The samples were treated as previously reported [11]. An equal volume of 0.35 M perchloric acid with 0.1 mg/ml EDTA and 0.2 mM glutathione was added to the aqueous sample and then filtered through a 0.22-Ìm membrane by centrifugation (UFC30GV00, Millipore).…”

Section: Measurement Of Asamentioning

confidence: 99%

Ultraviolet B-Induced Corneal and Lens Damage in Guinea Pigs on Low-Ascorbic Acid Diet

Wu¹,

Kojima²,

Shui³

et al. 2004

Ophthalmic Res

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To evaluate ocular morphological response to ultraviolet B (UV-B) exposure in guinea pigs fed with normal and low-ascorbic acid (ASA) diets, 24 guinea pigs were randomly divided into UVR (normal diet + UV-B), LVC (low-ASA diet + UV-B) and CTL (normal diet, without UV-B) groups. The irradiated animals were exposed to 8.2 J/cm² of UV-B over 29 weeks. Changes in the cornea and lens were examined using a Scheimpflug camera, specular microscope and histological studies. ASA contents in the plasma, aqueous humor and lens sections were measured by high-performance liquid chromatography. The results showed low contents of ASA in the plasma, aqueous humor and lens sections in LVC. Both irradiated groups displayed corneal epithelial edema, neovascularization in stroma, increase in corneal thickness, decrease in endothelial density, and lens epithelial damage. No significant morphological differences in cornea and lens were found between guinea pigs fed with low-ASA or normal diet.

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Section: Measurement Of Asamentioning

confidence: 99%

Ultraviolet B-Induced Corneal and Lens Damage in Guinea Pigs on Low-Ascorbic Acid Diet

Wu¹,

Kojima²,

Shui³

et al. 2004

Ophthalmic Res

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“…2.8. Determination of AA levels Ascorbic acid (AA) levels were estimated as previously described (Berger et al, 1989). In brief, homogenized (on ice in 0.35 M perchloric acid) samples were centrifuged for 3 min at 15,000 × g. A 400-μL aliquot of either the standard (AA) or sample was mixed with 100 μL of color reagent in a centrifugal analyzer tube.…”

Section: Determination Of Gsh Levelsmentioning

confidence: 99%

Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats

Dindar¹,

Kaltalıoğlu²,

Cevher³

2017

Turk J Zool

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Wound healing is a complex process affected by various conditions, including oxidative stress. The present study explored the time-dependent effects of platelet-derived growth factor (PGDF) and vascular endothelial growth factor (VEGF) administration on oxidative markers during acute stage wound healing. Thirty-six Wistar rats were distributed into three major groups; skin wounds were inflicted in all groups. The wounds were either left untreated (control group), treated topically with blank chitosan gel (blank chitosan gel group), or treated topically with a combination of PDGF and VEGF in chitosan gel (PDGF + VEGF chitosan gel group). Wounds were sampled on postsurgery days 3 and 7; samples were assayed for the oxidant markers nitric oxide (NOx) and thiobarbituric acidreactive substances (TBARs) and the antioxidant markers glutathione (GSH), ascorbic acid (AA), and superoxide dismutase (SOD) activity. PDGF + VEGF administration increased and decreased NOx levels on days 3 and 7, respectively. PDGF + VEGF administration lowered TBARs levels, compared with blank chitosan gel administration, on day 7. PDGF + VEGF administration increased GSH levels. These results demonstrate that PDGF + VEGF administration changes oxidative status of wound tissue. This study provides valuable insights for the development of therapeutic targets that promote wound healing.

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“…The average daily as corbate supplementation was 0.9 mg. All unopened bags of diet were frozen at -2 0 0 C to ensure that ascor bate did not deteriorate as a result of storage. To ensure consistency and accuracy of the animals' daily ascorbate intake, each bag of guinea pig diet was ana lyzed for reduced and total vitamin C content utilizing methods described by Berger et al [ 12] and Roe and Keuther [ 17], All guinea pigs were provided free access to drinking water treated by reverse osmosis, double ion exchange, and ultraviolet irradiation.…”

Section: Dietsmentioning

confidence: 99%

“…The Low-C diet (~ 5 mg/day) provided di etary levels of ascorbate, which were adequate to prevent scurvy, while the High-C diet (74 mg/day) provided more than ten times the daily recommended requirement for guinea pigs [ 19], This is enough to saturate all tissues in the parental strain with ascorbate [11,12], Ascorbate levels were evaluated at the conclu sion of the experiment to determine the bio availability of ascorbate in organs of interest. Table 1 shows that the diets resulted in two groups of hairless animals with different as corbate status.…”

Section: Tissue Ascorbate Levelsmentioning

confidence: 99%

“…This also presented an opportunity to use guinea pigs as a model for cataract. With cataractous guinea pigs, it would be possible to test relationships between ascorbate and cata ract, since (1) guinea pigs are one of the few laboratory animals that, like humans, require ascorbate, and (2) ascorbate levels in guinea pig tissues (including eye) can be regulated by dietary intervention [11,12], Furthermore, these relationships are of interest, since pre liminary data indicate that enhanced dietary ascorbate is associated with diminished cata ractlike damage in guinea pigs [6], with de layed cataract in selenite-stressed mice [13], and with diminished cataract risk in humans [14,15]. Thus, the acetone cataract model appeared a very attractive tool.…”

Section: Introductionmentioning

confidence: 99%

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Relationships between Acetone, Cataracts, and Ascorbate in Hairless Guinea Pigs

Taylor¹,

Smith²,

Palmer³

et al. 1993

Ophthalmic Res

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Acetone is one of the most commonly used industrial solvents. Recent literature indicated that in guinea pigs, but not rabbits, acetone is cataractogenic and that elevated acetone exposure is also associated with depressed aqueous ascorbate levels. Other work indicated that aqueous and lens levels of ascorbate are closely linked and that depressed ascorbate status is related to cataract. Taken together, these papers suggested that acetone exposure, depressed ascorbate levels, and cataract are related, possibly causally. While the possibility that acetone is cataractogenic presented a major health concern, it also presented an opportunity to develop a new model of cataract in which hypotheses regarding anticataractogenic effects of ascorbate could be tested. Albino hairless guinea pigs are immunocompetent animals derived from albino Hartley guinea pigs. Animals were fed diets containing low (4.9 mg/day) and high (55 mg/day) levels of ascorbate. This resulted in distinct groups of animals, one with high tissue ascorbate levels and the other with low, but nonscorbutic ascorbate levels. The tissue levels of ascorbate and the relationship between tissue ascorbate levels and dietary intake indicate that with respect to ascorbate uptake, transport, and concentration, these animals are identical to the standard albino Hartley animals. Daily exposure to acetone was extended for 6 months, with a total applied dose of 65 ml. Absorption of the solvent was maximized by the use of hairless animals. Despite exposure of the animals to higher levels of acetone, in no case (n = 20) were cataracts observed over a 2-year period. This is consistent with results using rabbits. The data show that the hairless guinea pig is a fine model for studies in which ascorbate bioavailability is of importance. However, other models to test the cataractogenic potential of acetone and anticataract effects of ascorbate are needed.

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Relationship between Dietary Intake and Tissue Levels of Reduced and Total Vitamin C in the Nonscorbutic Guinea Pig

Cited by 84 publications

References 25 publications

Ultraviolet B-Induced Corneal and Lens Damage in Guinea Pigs on Low-Ascorbic Acid Diet

Ultraviolet B-Induced Corneal and Lens Damage in Guinea Pigs on Low-Ascorbic Acid Diet

Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats

Relationships between Acetone, Cataracts, and Ascorbate in Hairless Guinea Pigs

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