Effect of dual growth factor administration on oxidative markers during acute stage wound healing in rats

Abstract

Wound healing is a complex process affected by various conditions, including oxidative stress. The present study explored the time-dependent effects of platelet-derived growth factor (PGDF) and vascular endothelial growth factor (VEGF) administration on oxidative markers during acute stage wound healing. Thirty-six Wistar rats were distributed into three major groups; skin wounds were inflicted in all groups. The wounds were either left untreated (control group), treated topically with blank chitosan gel (blank chitosan gel group), or treated topically with a combination of PDGF and VEGF in chitosan gel (PDGF + VEGF chitosan gel group). Wounds were sampled on postsurgery days 3 and 7; samples were assayed for the oxidant markers nitric oxide (NOx) and thiobarbituric acidreactive substances (TBARs) and the antioxidant markers glutathione (GSH), ascorbic acid (AA), and superoxide dismutase (SOD) activity. PDGF + VEGF administration increased and decreased NOx levels on days 3 and 7, respectively. PDGF + VEGF administration lowered TBARs levels, compared with blank chitosan gel administration, on day 7. PDGF + VEGF administration increased GSH levels. These results demonstrate that PDGF + VEGF administration changes oxidative status of wound tissue. This study provides valuable insights for the development of therapeutic targets that promote wound healing.

Comparison of Oxidative Effects of Two Different Administration Form of Oxybutynin in the Potential Target Tissues

“…NOx are stable end products of nitric oxide. Sodium nitrite was considered as the standard [18]. Lipid peroxidation was determined spectrophotometrically according to the method detailed before at 535 nm.…”

“…This method based on the principle that the generation of malondialdehyde (MDA) as an end product of lipid peroxidation, which interacts with thiobarbituric acid producing thiobarbituric acid-reactive substance (TBARs). Tetraethoxypropane solution was considered as a standard for this assay [18]. Levels of glutathione (GSH) were measured spectrophotometrically according to a modified version of the Ellman method at 412 nm.…”

“…Levels of glutathione (GSH) were measured spectrophotometrically according to a modified version of the Ellman method at 412 nm. In this method, 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB) reacts with reduced GSH to form a yellow-colored compound [18].…”

Advancements in the Delivery of Growth Factors and Cytokines for the Treatment of Cutaneous Wound Indications

“…A chitosan hydrogel encapsulating PDGF-bb and VEGF-A improved the oxidative status of sutured incisions relative to hydrogel-treated controls. 164 Most GF and cytokine delivery systems have, however, been evaluated in acute full-thickness excisional wounds, either in the rat or mouse.…”

“…Combination therapies evaluated for cutaneous wound and scarring indications Rat full-thickness incisional wound model (sutured)-single treatment led to oxidative changes relative to hydrogel and untreated controls164 …”