Regulation of polyurethane hemocompatibility and endothelialization by tethered hyaluronic acid oligosaccharides

The effect of coimmobilizing heparin and fibronectin on titanium on hemocompatibility and endothelialization

“…Heparin with the density of 2.6 mg/cm 2 on PU surface was found to significantly inhibit platelets adhesion [28], thus in our study, after PBS immersion for five days, the residual heparin was still enough to exhibit the anticoagulant properties. However, the amount of physisorbed heparin on Ti surface dramatically decreased to an extremely low level even at the 1st day, which was equivalent to that on TiOHA surface without heparin adsorption, and further decreased to a level equivalent to Ti BLK (blank sample), indicating a through loss of heparin on physisorbed Ti surface.…”

“…We may speculate from the release difference of the two biomolecules that combination of heparin and fibronectin by electrostatic interaction was disturbed after immersion, and the stability of heparin and fibronectin depended on several different elements, including the concentration, the molecular weight and structure, and the integration degree with the substrate, etc. Though there was release of heparin and fibronectin from the Hep/Fn coimmobilized films, it was still reasonable to believe that the Hep/Fn complex was stable enough for performing its biofunctionality in terms of the residual amount and the studies by others [28].…”

Extract of fetal membrane would inhibit thrombosis and hemolysis

“…An approach to solve the problem is employing native biomolecules to modify the surface, for example some native biomolecules such as glycosaminoglycans are hired to regulate thrombogenicity and cell-adhesion [19]. Heparin is a glycosaminoglycan that inhibits platelet adhesion and aggregation by preventing the release of serotonin and thromboxane-A2 from platelets and also has anticoagulant activity by binding to anti-thrombin III.…”

Strategies and Techniques to Enhance theIn SituEndothelialization of Small-Diameter Biodegradable Polymeric Vascular Grafts

“…92 More recently, hyaluronic acid (HA) oligosaccharide, a glycosaminoglycan with angiogenic and antithrombogenic properties, chains of varying lengths were grafted to PU-based films. 93 While all three different lengths of HA proved more effective at limiting platelet adhesion and protein adsorption than PEG-or heparin-modified PU films, it was found that endothelial growth on the films was dependent on the molecular weight of the HA chains. Smaller chains of HA appeared to support better EC growth.…”