Regulation of oncogenic genes by MicroRNAs and pseudogenes in human lung cancer

Tutar, Yusuf; Özgür, Aykut; Tutar, Esen; Tutar, Lütfi; Pulliero, Alessandra; Izzotti, Alberto

doi:10.1016/j.biopha.2016.08.043

Cited by 28 publications

(14 citation statements)

References 73 publications

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“…It is well-known that numerous genes are responsible for carcinogenesis and cancer development. Therefore, miRNAs can act as tumor suppressors or oncogenes, by binding to regulators associated with cancer cell survival, proliferation, apoptosis and metastasis (25,26). A growing body of evidence has demonstrated that miRNA-145 serves a suppressive role in various cancers (27)(28)(29)(30).…”

Section: Discussionmentioning

confidence: 99%

microRNA‑145 modulates migration and invasion of bladder cancer cells by targeting N‑cadherin

Zhang

Chang

et al. 2018

Mol Med Report

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MicroRNA (miRNA)‑145 has been demonstrated to serve a role in several types of tumors, however, the potential molecular mechanism of action of miRNA‑145 in bladder cancer metastasis remains to be elucidated. This study aimed to investigate the potential modulation of miRNA‑145 in bladder carcinoma and elucidate the underlying molecular mechanism. The expression of miRNA‑145 in bladder adenocarcinoma tissues and bladder cancer cells was measured by reverse transcription‑quantitative polymerase chain reaction. miRNA‑145 mimics and inhibitor were transfected into bladder cancer (BC) cells to determine the role of miRNA‑145 on cell motility and invasion measured by wound healing and transwell assays. Luciferase assay was performed to confirm whether N‑cadherin was the direct target of miRNA‑145. Subsequently, expression of N‑cadherin and matrix metalloproteinase‑9 (MMP9) in BC cells were detected by western blot analysis. miRNA‑145 was significantly downregulated cells and tissues from patients with BC, compared with healthy controls. miRNA‑145 markedly inhibited the ability of BC cells to migrate and invade. Furthermore, N‑cadherin was identified as a target of miRNA‑145 in BC cells. MMP9, acting downstream of N‑cadherin, was downregulated in BC cells by miRNA‑145. In the present study, miRNA‑145 suppressed the migration and invasion of BC cells by regulating N‑cadherin. The results of the present study indicated that miRNA‑145 may function as a tumor suppressor and may have a potential to be a diagnostic and predictive biomarker, and a therapeutic target for treatment of BC.

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Section: Discussionmentioning

confidence: 99%

microRNA‑145 modulates migration and invasion of bladder cancer cells by targeting N‑cadherin

Zhang

Chang

et al. 2018

Mol Med Report

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“…Although pseudogenes were classified as non-functioning genomic fossils initially in 1977 [ 10 ], pseudogene-derived RNAs can function as antisense RNAs (asRNAs), endogenous siRNAs, and competing endogenous RNAs (ceRNAs) during post-transcriptional regulation [ 11 ]. Moreover, many studies have verified that several pseudogenes participate in multiple biological processes, and their deregulation is associated with human diseases, especially cancers [ 12–14 ]. Long non-coding RNAs (lncRNAs) are defined as transcripts that are longer than 200 nts and do not encode proteins [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

The pseudogene-derived long non-coding RNA SFTA1P suppresses cell proliferation, migration, and invasion in gastric cancer

Chen

et al. 2018

Bioscience Reports

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Pseudogenes were once regarded as transcriptionally inactive and without specific molecular function. However, current evidence shows that pseudogene-derived long non-coding RNAs (lncRNAs) may be crucial regulators of human cancer development, including gastric cancer (GC). In the present study, we report that a pseudogene-derived lncRNA named surfactant associated 1, pseudogene (SFTA1P), which is 693-nt long, was significantly down-regulated in GC tissues compared with that in the adjacent normal tissues. In addition, decreased SFTA1P expression was strongly correlated with advanced tumor lymph node metastasis (TNM) stage, larger tumor size, lymphatic metastasis, and poor prognosis of patients with GC. Moreover, gain-of-function experiments revealed that the overexpression of SFTA1P inhibits cell proliferation, migration, and invasion, thus verifying the tumor inhibitory role of SFTA1P in GC. Furthermore, we investigated the potential action mechanism of SFTA1P. Our results showed that down-regulation of SFTA1P may be associated with decreased TP53 expression. In summary, our work suggests that the pseudogene-derived lncRNA SFTA1P functions as a tumor suppressor in GC and thus may act as a potential diagnostic and therapeutic target of GC.

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“…Microarray technology has allowed detection of pseudogene transcription at the whole genome level in human malignancy and has shown that several pseudogenes are transcribed. Also, increasing numbers of studies have confirmed that several pseudogenes are involved in a variety of biological processes, and their deregulation may participate in human diseases, including human cancer [9–11].…”

Section: Introductionmentioning

confidence: 99%

The Octamer-Binding Transcription Factor 4 (OCT4) Pseudogene, POU Domain Class 5 Transcription Factor 1B (POU5F1B), is Upregulated in Cervical Cancer and Down-Regulation Inhibits Cell Proliferation and Migration and Induces Apoptosis in Cervical Cancer Cell Lines

Zhang

Zhou

et al. 2019

Med Sci Monit

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Background The POU domain class 5 transcription factor 1B (POU5F1B), is a pseudogene that is homologous to octamer-binding transcription factor 4 (OCT4), and is located adjacent to the MYC gene on human chromosome 8q24. POU5F1B has been reported to be transcribed in several types of cancer, but its role in cervical cancer remains unclear. This study aimed to investigate the expression and function of POU5F1B in tissue samples of human cervical cancer and in cervical cancer cell lines in vitro . Material/Methods Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to quantify POU5F1B expression in cervical cancer tissues and in SiHa, HeLa, CaSki, and C33A human cervical cancer cell lines. Functional in vitro studies included analysis of the effects of POU5F1B expression on cervical cancer cell proliferation, migration, and apoptosis using a Cell Counting Kit-8 (CCK-8) assay, cell migration assays, and flow cytometry. Luciferase activity assays, qRT-PCR, and Western blot were performed to confirm the expression of POU5F1B. Results POU5F1B was significantly upregulated in cervical cancer tissues and cell lines. Interference with the expression of POU5F1B significantly inhibited cell proliferation, apoptosis, migration and invasion, and induced apoptosis in vitro . Western blot demonstrated that POU5F1B could modulate the expression of the OCT4 protein. Conclusions POU5F1B was upregulated in cervical cancer and down-regulation inhibited cell proliferation and migration and induced apoptosis in cervical cancer cell lines by modulating OCT4. Further studies are required to determine whether POU5F1B might be a diagnostic or prognostic biomarker or therapeutic target in cervical cancer.

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Regulation of oncogenic genes by MicroRNAs and pseudogenes in human lung cancer

Cited by 28 publications

References 73 publications

microRNA‑145 modulates migration and invasion of bladder cancer cells by targeting N‑cadherin

microRNA‑145 modulates migration and invasion of bladder cancer cells by targeting N‑cadherin

The pseudogene-derived long non-coding RNA SFTA1P suppresses cell proliferation, migration, and invasion in gastric cancer

The Octamer-Binding Transcription Factor 4 (OCT4) Pseudogene, POU Domain Class 5 Transcription Factor 1B (POU5F1B), is Upregulated in Cervical Cancer and Down-Regulation Inhibits Cell Proliferation and Migration and Induces Apoptosis in Cervical Cancer Cell Lines

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