The Octamer-Binding Transcription Factor 4 (OCT4) Pseudogene, POU Domain Class 5 Transcription Factor 1B (POU5F1B), is Upregulated in Cervical Cancer and Down-Regulation Inhibits Cell Proliferation and Migration and Induces Apoptosis in Cervical Cancer Cell Lines

Yu, Jingwen; Zhang, Jingling; Zhou, Liulin; Li, Hao; Deng, Zhaoqun; Meng, Bi

doi:10.12659/msm.912109

Cited by 13 publications

(4 citation statements)

References 33 publications

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“…Worldwide, cervical cancer is the second most common malignancy in women, after breast cancer, and has a high mortality rate [1–3]. Currently, there are many treatment approaches, including surgery, chemotherapy, and radiotherapy, but these are not always satisfactory, particularly in late-stage cervical cancer [4].…”

Section: Introductionmentioning

confidence: 99%

Long Noncoding RNA TP73-AS1 Targets MicroRNA-329-3p to Regulate Expression of the SMAD2 Gene in Human Cervical Cancer Tissue and Cell Lines

et al. 2019

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Departmental sources Background: Worldwide, mortality from cervical cancer in women remains high. This study aimed to investigate the expression of long noncoding RNA (lncRNA) TP73-AS1, microRNA-329-3p (miRNA-329-3p), and the SMAD2 gene and their regulatory relationships in human cervical cancer tissue and cervical cancer cell lines. Material/Methods: Cervical cancer tissue samples (n=30) and normal control cervical tissues were studied. Cell proliferation and migration were investigated in HeLa and SiHa human cervical cancer cells using the MTT assay, crystal violet staining, wound healing assay, and the transwell assay. Expression of lncRNA TP73-AS1 and the SMAD2 gene were detected using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Enrichment of miR-329-3p was measured using the RNA immunoprecipitation assay (RIPA). Targeting relationships between TP73-AS1, miR-329-3p, and SMAD2 were identified using the dual-luciferase reporter assay. A subcutaneous xenograft model was established, tumor size was measured, and SMAD2 expression was detected using immunohistochemistry. Results: LncRNA TP73-AS1 was overexpressed in cervical cancer tissues and cells and was associated with reduced expression of miR-329-3p. Down-regulation of lncRNA TP73-AS1 inhibited cell proliferation, migration and invasion and increased miR-329-3p expression. Expression of SMAD2 down-regulated miR-329-3p and was associated with increased expression of TP73-AS1. LncRNA TP73-AS1 knockdown resulted in miR-329-3p silencing. In tumor xenografts, expression of TP73-AS1 reduced the tumor volume and down-regulated the expression levels of the SMAD2 gene. Conclusions: LncRNA TP73-AS1 promoted proliferation of cervical cancer cell lines by targeting miR-329-3p to regulate the expression of the SMAD2 gene. A regulatory network was formed between lncRNA TP73-AS1, miR-329-3p, and SMAD2.

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Section: Introductionmentioning

confidence: 99%

Long Noncoding RNA TP73-AS1 Targets MicroRNA-329-3p to Regulate Expression of the SMAD2 Gene in Human Cervical Cancer Tissue and Cell Lines

et al. 2019

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“…Recently, Liu et al (2017c) showed that RSU1P2 was increased in cervical cancer and boosted the malignant phenotype of cervical cancer. POU5F1B was also reported to be upregulated in cervical cancer tissues and cell lines by Yu et al (2019). The authors confirmed that interference with expression of POU5F1B significantly suppressed cell proliferation, migration and invasion, and induced apoptosis in cervical cancer .…”

Section: Other Types Of Cancermentioning

confidence: 69%

Pseudogene-Derived lncRNAs and Their miRNA Sponging Mechanism in Human Cancer

Lou

Ding

2020

Front. Cell Dev. Biol.

124

115

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“…GBP1P1 and PTTG3P were observed in microarray analysis and validated by qRT-PCR in tissues of cervical carcinoma [46]. FTH1P3 and POU5F1B are upregulated in cervical cancer patient samples and cell lines [47,48]. In colon cancer tissues, DUXAP8, RP11-54H7.4, and RP11-138J23.1 show elevated expression in advanced tumor stages [49].…”

Section: Cancers Located In the Abdomen And Bonesmentioning

confidence: 82%

The World of Pseudogenes: New Diagnostic and Therapeutic Targets in Cancers or Still Mystery Molecules?

Stasiak

Kolenda

Kozłowska-Masłoń

et al. 2021

Life

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Pseudogenes were once considered as “junk DNA”, due to loss of their functions as a result of the accumulation of mutations, such as frameshift and presence of premature stop-codons and relocation of genes to inactive heterochromatin regions of the genome. Pseudogenes are divided into two large groups, processed and unprocessed, according to their primary structure and origin. Only 10% of all pseudogenes are transcribed into RNAs and participate in the regulation of parental gene expression at both transcriptional and translational levels through senseRNA (sRNA) and antisense RNA (asRNA). In this review, about 150 pseudogenes in the different types of cancers were analyzed. Part of these pseudogenes seem to be useful in molecular diagnostics and can be detected in various types of biological material including tissue as well as biological fluids (liquid biopsy) using different detection methods. The number of pseudogenes, as well as their function in the human genome, is still unknown. However, thanks to the development of various technologies and bioinformatic tools, it was revealed so far that pseudogenes are involved in the development and progression of certain diseases, especially in cancer.

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The Octamer-Binding Transcription Factor 4 (OCT4) Pseudogene, POU Domain Class 5 Transcription Factor 1B (POU5F1B), is Upregulated in Cervical Cancer and Down-Regulation Inhibits Cell Proliferation and Migration and Induces Apoptosis in Cervical Cancer Cell Lines

Cited by 13 publications

References 33 publications

Long Noncoding RNA TP73-AS1 Targets MicroRNA-329-3p to Regulate Expression of the SMAD2 Gene in Human Cervical Cancer Tissue and Cell Lines

Long Noncoding RNA TP73-AS1 Targets MicroRNA-329-3p to Regulate Expression of the SMAD2 Gene in Human Cervical Cancer Tissue and Cell Lines

Pseudogene-Derived lncRNAs and Their miRNA Sponging Mechanism in Human Cancer

The World of Pseudogenes: New Diagnostic and Therapeutic Targets in Cancers or Still Mystery Molecules?

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