Regulation of expression and role of leukemia inhibitory factor and interleukin-6 in the uterus of early pregnant pigs

Blitek, Agnieszka; Morawska, E.; Ziȩcik, Adam J.

doi:10.1016/j.theriogenology.2012.05.016

Cited by 57 publications

(62 citation statements)

References 40 publications

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“…Moreover, level of LIF mRNA on Day 12 was much higher in pregnancy than in the estrous cycle ( p < 0.01). These data corresponded with the results of the previous studies [3], showing that LIF expression in the endometrium tended to be higher on Day 12 of pregnancy compared with non-pregnant animals. The increased LIF expression on Day 12 of pregnancy coincides with an abundance of estrogens in the uterine lumen [13].…”

Section: Resultssupporting

confidence: 92%

“…Profile of endometrial expression of LIFR mRNA on Day 14 of pregnancy vs. the estrous cycle is in agreement with the results of the recently performed microarray analysis of the endometrial transcriptomes [14]. The increased expression of the LIF and LIFR in the porcine endometrium on Days 12-16 of pregnancy (present data) coincides with elevated LIF content in the uterine lumen (Day 12) and abundant expression of LIFR mRNA in the porcine conceptuses (Day 14) [3], which confirms the important role of this system during maternal recognition of pregnancy and implantation.…”

Section: Resultssupporting

confidence: 91%

“…The increased LIF expression on Day 12 of pregnancy coincides with an abundance of estrogens in the uterine lumen [13]. Thus, LIF synthesis in the pig endometrium is induced during the maternal recognition of pregnancy (Day 12) to support the conceptus development and attachment [3]. The expression of LIFR mRNA was affected by day ( p < 0.0001) and the reproductive status ( p = 0.005; Fig.…”

Section: Resultsmentioning

confidence: 99%

“…This family comprises several molecules, including IL6, IL11, leukemia inhibitory factor (LIF) and ciliary neurotrophic factor (CNTF) [for review see : 1]. LIF is a cytokine which seems to play a pivotal role in the regulation of endometrial functions and embryo implantation and has been identified in the uterus of many species, such as humans [2] and pigs [3,4]. Both LIF and IL6 are present in the uterine environment during early pregnancy of the pig, affecting endometrial function as well as the attachment and proliferation of trophoblast cells [4].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Differential expression of genes linked to the leukemia inhibitor factor signaling pathway during the estrus cycle and early pregnancy in the porcine endometrium

Kaczmarek

Krawczynski

Najmula

et al. 2014

Reproductive Biology

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Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Differential expression of genes linked to the leukemia inhibitor factor signaling pathway during the estrus cycle and early pregnancy in the porcine endometrium

Kaczmarek

Krawczynski

Najmula

et al. 2014

Reproductive Biology

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“…There is an up-regulation expression of endometrial IL-6 mRNA during early pregnancy in pigs, and IL-6 can stimulate the attachment and proliferation of trophoblast cells in vitro, which implies that IL-6 contributes to successful implantation of conceptus in pig (Blitek et al, 2012). It is essential for normal placenta development and successful pregnancy that IL-6 is produced by extravillous and cytotrophoblast (Goyal et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Changes in expression of Th1 and Th2 cytokines in bovine peripheral blood mononuclear cells during early pregnancy

Yang¹,

Wang²,

Xingshu³

et al. 2015

IJAR

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Conceptus-derived signals are involved in the maternal systemic immune regulation through blood circulation and lymph circulation by peripheral blood mononuclear cells (PBMC) in cattle. There exists a Th2-biased response in maternal uterus during early pregnancy in cattle. However, it is unclear that the changes in expression of Th1 and Th2 cytokines in PBMC during early pregnancy in cows. At present study, the expression of Th1 and Th2 cytokines in PBMC were explored at day 18 non-pregnant cows, day 18 and day 30 pregnant cows. Our results showed that IFN- was attenuated in PBMC, but TNF- and IL-2 in PBMC were up-regulated during early pregnancy in cows. However, the member of the Th2 cytokines, including IL-4, IL-5, IL-6, IL-10 and IL-13, was augmented in day 30 pregnant PBMC. In conclusion, there existed a Th2-biased response in PBMC during early pregnancy, which was essential for successful pregnancy in cattle.

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Effects of seminal plasma and the presence of a conceptus on regulation of lymphocyte‐cytokine network in porcine endometrium

Jalali

Kitewska

Wasielak

et al. 2014

Molecular Reproduction Devel

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Infusion of seminal plasma in the uterus is known to elicit an instant inflammatory response in the porcine uterus, but whether or not it prepares a uterine immunological response to the presence of conceptuses is not well understood. Seminal plasma induced long-term modulatory effects and conceptus-induced immune changes in leukocyte populations were measured by flow cytometry and mRNAs for various cytokines by quantitative reverse-transcriptase PCR in porcine endometrium collected on Days 6 and 13 from cycling and pregnant animals or from animals given seminal plasma infusions. Seminal plasma infusion induced long-term modulatory effects, resulting in significantly more endometrial FoxP3-positive T-regulatory and T-helper cells 6 days after infusion as compared to cycling and pregnant animals. The number of T-cytotoxic and T-null cells did not change between the studied groups. The early molecular effects of seminal plasma were not observed at 13-days post-infusion, although animals on Day 13 of pregnancy did show significantly more T-cells (of any type investigated). Seminal plasma also showed a delayed effect on cytokine expression, specifically exhibiting a significant increase in interleukin 10 (IL10) and a decrease in granulocyte macrophage colony-stimulating factor (GMCSF) gene expression on Day 13 as compared to Day 6 of cycling or pregnant gilts. The results indicate a delayed regulatory effect of seminal plasma on immune responses in the porcine uterus, which are similar to immune changes generated by implanting conceptuses.

show abstract

Regulation of expression and role of leukemia inhibitory factor and interleukin-6 in the uterus of early pregnant pigs

Cited by 57 publications

References 40 publications

Differential expression of genes linked to the leukemia inhibitor factor signaling pathway during the estrus cycle and early pregnancy in the porcine endometrium

Differential expression of genes linked to the leukemia inhibitor factor signaling pathway during the estrus cycle and early pregnancy in the porcine endometrium

Changes in expression of Th1 and Th2 cytokines in bovine peripheral blood mononuclear cells during early pregnancy

Effects of seminal plasma and the presence of a conceptus on regulation of lymphocyte‐cytokine network in porcine endometrium

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