Differential expression of genes linked to the leukemia inhibitor factor signaling pathway during the estrus cycle and early pregnancy in the porcine endometrium

Kaczmarek, Monika M.; Krawczynski, Kamil; Najmula, Joanna; Reliszko, Zaneta P.; Sikora, Małgorzata; Gajewski, Zdzisław

doi:10.1016/j.repbio.2014.06.003

Cited by 8 publications

(12 citation statements)

References 14 publications

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“…Interestingly, addition of conceptus‐conditioned culture medium elevates the concentrations of IL6 and LIF in endometrial tissue explants (Blitek et al, ), indicating conceptus‐triggered regulation of their expression. These cytokines also increase the viability and adhesion of porcine trophoblast cells in vitro (Blitek et al, ), which is consistent with the detection of LIF and IL6 receptors on the porcine conceptus (Blitek et al, ; Kaczmarek et al, ; Modrić et al, ). Although the receptors are also present on the porcine endometrium (Blitek et al, ; Kaczmarek et al, ; Modrić et al, ), the outcome of their signaling in this tissue is not understood.…”

Section: The Conceptus‐maternal Interface and The Pro‐inflammatory Ensupporting

confidence: 72%

Embryo‐maternal dialogue during pregnancy establishment and implantation in the pig

Wacławik

Kaczmarek

Blitek

et al. 2017

Molecular Reproduction Devel

Self Cite

102

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Porcine conceptuses secrete pregnancy-recognition signals (estrogens, including estradiol-17β) that inhibit luteolysis, thereby prolonging progesterone production by corpora lutea. The supportive mechanism by which the conceptus also inhibits luteolysis is by shifting endometrial prostaglandin (PG) synthesis to luteoprotective PGE2.Progesterone stimulates endometrial production of factors that are essential for conceptus development. Priming the uterus by progesterone and loss of progesterone receptors from the uterine epithelium by Day 10-12 after estrus are key for achieving endometrial receptivity for implantation. Conceptus implantation involves a series of events, many resembling the inflammatory reaction, that are greatly influenced by cytokines, growth factors, and prostaglandins. We herein present a novel, dual role for PGF2α in corpora lutea that depends on the acquisition of luteolytic sensitivity, based on the knowledge that PGF2α triggers pathways involved in luteolysis during the estrous cycle or/and may have an alternative function in maintaining progesterone synthesis during pregnancy. We also point out a new role for PGF2α that, together with PGE2, can act as embryonic signal mediators. PGF2α, which until recently was considered undesirable for promoting pregnancy, is now known to stimulate conceptus-maternal interactions and angiogenesis in the endometrium. This function is in line with other important prostaglandin functions, such as stimulating adhesion of trophoblasts (PGE2, PGI2) as well as endometrial vascular functions and trophoblast cell proliferation (PGI2).

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Section: The Conceptus‐maternal Interface and The Pro‐inflammatory Ensupporting

confidence: 72%

Embryo‐maternal dialogue during pregnancy establishment and implantation in the pig

Wacławik

Kaczmarek

Blitek

et al. 2017

Molecular Reproduction Devel

Self Cite

102

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“…LIF plays a crucial functional role in stimulating the proliferation and attachment of porcine trophoblast cells during early pregnancy (Blitek et al, ). In addition, the mRNA expression of LIFR can be varied on different days during embryo implantation and is affected by the reproductive status during early pregnancy in pigs (Kaczmarek et al, ). Similarly, the expression of EGF family members and their receptors increases in reproductive organs during the peri‐implantation and implantation period in pigs (Kennedy et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…LIF plays a crucial functional role in stimulating the proliferation and attachment of porcine trophoblast cells during early pregnancy (Blitek et al, 2012). In addition, the mRNA expression of LIFR can be varied on different days during embryo implantation and is affected by the reproductive status during early pregnancy in pigs (Kaczmarek et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Gene expression in the embryo and maternal environment could affect embryonic development potential and embryo implantation during early pregnancy (Bauer et al, ; Fernandez‐Gonzalez et al, ; Kepkova et al, ; Patricia Fauque et al, ). Previous studies have reported that several molecules could have an effect on endometrium and embryo implantation during early pregnancy, such as LIF (Ropka‐Molik, Oczkowicz, Mucha, Piórkowska, & Piestrzyńska‐Kajtoch, ), LIF receptor ( LIFR ; Kaczmarek et al, ), integrins (Lessey, ), l‐selectin (Genbacev et al, ) and EGF (Kennedy, Brown, & Vaughan, ). Collectively, identification of the molecules that may have an effect on porcine endometrial cells during early pregnancy is important for understanding the implantation of blastocyst and embryo development in pigs.…”

Section: Introductionmentioning

confidence: 99%

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Annexin A8 (ANXA8) regulates proliferation of porcine endometrial cells via Akt signalling pathway

Jiang

Xue

Kang

et al. 2018

Reprod Domestic Animals

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Annexin A8 (ANXA8) gene, a member of the annexin family, encodes an anticoagulant protein involved in blood coagulation cascade and acts as an indirect inhibitor of the thromboplastin-specific complex. However, little is known about the function of ANXA8 in porcine endometrial cells so far. Here, ANXA8 mRNA was found to be abundant in porcine endometrium on days 11-13 of pregnancy. Real-time RT-PCR analysis indicated that the mRNA expression of the leukaemia inhibitory factor (LIF) and the epidermal growth factor (EGF) was upregulated by ANXA8 in porcine endometrial cells. Immunofluorescence technology and cell cycle analysis revealed that ANXA8 promoted the proliferation of endometrial cells, as evidenced by the abundant proliferating cell nuclear antigen (PCNA) expression and an increase in the S phase. Western blot analysis results indicated that ANXA8 activated the phosphorylation of the target protein kinase B (Akt) protein. Immunofluorescence technology results showed that the PCNA protein had no significant change in porcine endometrial cells with both ANXA8 overexpression and the addition of Akt inhibitor. Furthermore, the number of implantation sites was significantly reduced by injection of mus-siRNA-ANXA8 into the uterine horn of mice. Collectively, these results suggest that ANXA8 promotes the proliferation of endometrial cells through the Akt signalling pathway.

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“…Many factors secreted within the pig uterus are important during the critical period of conceptus elongation and implantation, including insulin‐like growth factors (IGFs) (Green, Simmen, & Simmen, ; Jeong, Song, Bazer, & Kim, ), IGF‐binding proteins (Geisert, Chamberlain, Vonnahme, Malayer, & Spicer, ; Lee, Green, Simmen, & Simmen, ), epidermal growth factor (EGF) (Jeong, Jung, Bazer, Song, & Kim, ; Kennedy, Brown, & Vaughan, ; Vaughan, James, Pascall, & Brown, ), heparin binding‐EGF (Kim et al, ), transforming growth factor beta (TGFB) (Jaeger et al, ; Massuto et al, ), fibroblast growth factor 7 (FGF7) (Ka et al, , ), vascular endothelial growth factor (VEGF) (Kaczmarek et al, ), colony stimulating factor 2 (CSF2) (Jeong, Kim, Bazer, & Song, ), leukemia inhibitory factor (LIF) (Kaczmarek et al, ), and interleukin 6 (IL6) (Anegon et al, ; Modric, Kowalski, Green, Simmen, & Simmen, ). Endometrial secretion of FGF7, LIF, and IGF‐1 are specificity enhanced before and after elongation (reviewed by Geisert et al, ).…”

Section: Conceptus Elongation and Establishment Of Pregnancymentioning

confidence: 99%

Rapid conceptus elongation in the pig: An interleukin 1 beta 2 and estrogen‐regulated phenomenon

Geisert

Whyte

Meyer

et al. 2017

Molecular Reproduction Devel

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Establishment and maintenance of pregnancy in the pig involves activating many physiological, cellular, and molecular signaling pathways between the developing conceptus and hormonally regulated maternal endometrium. Rapid elongation of the pig trophoblast allows for the establishment of sufficient placental surface area for the transport of nutrients to the fetus throughout pregnancy. Estrogens secreted by the conceptus during elongation act on uterine epithelia to induce secretion of uterine factors required for conceptus development and for preventing endocrine secretion of prostaglandin F2α, which would cause luteolysis. Thus, trophoblast expansion within the uterine lumen during early gestation is an essential process for implantation and maintenance of pregnancy in species with an epitheliochorial form of placentation. In the pig, rapid conceptus elongation involves the unique expression of interleukin-1 beta 2 (IL1B2), which establishes pro-inflammatory effects that may be tempered by the spatiotemporal secretion of estrogen from the conceptuses. The present review provides current information on pig conceptus remodeling and signaling via estrogen and IL1B2 pathways, as well as endometrial responses to those conceptus factors leading to establishment of pregnancy.embryo, endometrium, placenta, pregnancy[The] unique capacity of pig conceptuses to rapidly remodel and elongate, compared with other mammals, is an essential mechanism developed to establish pregnancy.

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Differential expression of genes linked to the leukemia inhibitor factor signaling pathway during the estrus cycle and early pregnancy in the porcine endometrium

Cited by 8 publications

References 14 publications

Embryo‐maternal dialogue during pregnancy establishment and implantation in the pig

Embryo‐maternal dialogue during pregnancy establishment and implantation in the pig

Annexin A8 (ANXA8) regulates proliferation of porcine endometrial cells via Akt signalling pathway

Rapid conceptus elongation in the pig: An interleukin 1 beta 2 and estrogen‐regulated phenomenon

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