Regeneration through somatic embryogenesis from petal-derived calli of Rosa hybrida L. cv arizona (hybrid tea)

Murali, S.; Sreedhar, D.; Lokeswari, T. S.

doi:10.1007/bf00033087

Cited by 33 publications

(16 citation statements)

References 11 publications

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“…However, in our study, the highest frequency of maturation and germination of secondary somatic embryos was achieved on1/2-strength MS. This result is concordant with the finding in R. hybrida 'arizona' (Murali et al 1996).…”

Section: Discussionsupporting

confidence: 95%

An efficient system to produce transgenic plants via cyclic leave-originated secondary somatic embryogenesis in Rosa rugosa

et al. 2014

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An efficient and reproducible Agrobacteriummediated transformation system via repetitive secondary somatic embryogenesis was developed for Rosa rugosa 'Bao white'. Somatic embryogenesis was induced from in vitro-derived unexpanded leaflet explants on MS medium supplemented with 4.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), 0.05 mg/L Kinetin and 30 g/L glucose. Secondary somatic embryos were successfully proliferated via cyclic secondary somatic embryogenesis on MS medium containing 1.0 mg/L 2,4-D, 0.01 mg/L 6-benzyladenine and 45 g/L glucose under light intensity of 500-1,000 lux. The highest germination rate (86.33 %) of somatic embryos was observed on 1/2-strength MS medium containing 1.0 mg/L BA. Relying on the repetitive secondary somatic embryogenesis and A. tumefaciens strain EHA105 harboring the binary vector pBI121, a stable and effective Agrobacterium-mediated transformation pattern was developed. The presented transformation protocol, in which somatic embryo clumps at globular stage (0.02-0.04 g) were infected by Agrobacterium for 60 min and co-cultivated for 2 days, and then selected under a procedure of 3 steps, were confirmed to be optional by GUS histochemical assay and Southern blot analysis. The procedure described here will be very useful for the introgression of desired genes into R. rugosa 'Bao white' and the molecular analysis of gene function.

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Section: Discussionsupporting

confidence: 95%

An efficient system to produce transgenic plants via cyclic leave-originated secondary somatic embryogenesis in Rosa rugosa

et al. 2014

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“…Consequently, reproductive parts are seldom used as explants for shoot organogenesis. Callus from floral parts in many cases is embryogenic (Murali et al 1996;Kintzios and Michaelakis 1999) or produces adventitious floral organs (Orlikowska et al 1998;Wojciechowicz 2002), suggesting a retention of floral determination by the callus cells. In some cases, however, floral-derived callus can also undergo sufficient dedifferentiation to give rise to vegetative shoots (Heuser and Apps 1976), as seen in our Mammillaria cultures.…”

mentioning

confidence: 98%

Organogenesis of vegetative shoots from in vitro cultured flower buds of Mammillaria albicoma (Cactaceae)

Wyka

Hamerska

Wróblewska

2006

Plant Cell Tiss Organ Cult

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We tested the organogenetic capacity of floral buds of Mammillaria albicoma Bö ed. (Cactaceae). Buds were incubated on solid MS medium supplemented with 0.1 mg l -1 a-naphthaleneacetic acid (NAA) and 5.0 mg l -1 6-benzylaminopurine (BA). Callus growth was observed from the cut explant base and from within the perianth. These calli during subsequent subcultures to the same medium gave rise to adventitious shoots. Shoots formed also directly from the perianth, as confirmed by observations in the light microscope and scanning electron microscope (SEM). On transfer to a fresh medium, the shoots produced proliferating cultures. This is the first report of regeneration of cactus shoots from floral explants.

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“…Somatic embryos of the two floribundas, Trumpeter and Glad Tidings, converted to plants at a 9-17% frequency (Marchant et al 1996). The hybrid tea rose Royalty showed a 12% conversion rate, while another hybrid tea, Arizona, was reported to have a 38% maturation and a 93% conversion rate (Noriega and Sondahl 1991;Murali et al 1996). Kunitake et al (1993) found that somatic embryos of Rosa rugosa, a wild rose native to the northern regions of Asia, had a plant conversion frequency of 3%.…”

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 97%

“…For example, the maturation of somatic embryos was found by Murali et al (1996) to be affected by abscisic acid, while Marchant et al (1996) used abscisic acid combined with BAP. Phloroglucinol was required for the conversion of Arizona embryos (Murali et al 1996). Either BAP combined with indole-3-butyric acid and a 4C cold treatment (Marchant et al 1996) or methyl laurate and BAP (Sarasan et al 2001) were shown to stimulate conversion.…”

Section: Introductionmentioning

confidence: 97%

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Dispersal and size fractionation of embryogenic callus increases the frequency of embryo maturation and conversion in hybrid tea roses

et al. 2004

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Plant regeneration from embryogenic cells of two Rosa hybrida cultivars, Kardinal and Classy, was increased by dispersing embryogenic callus in liquid medium for 3 h followed by size-fractionation to isolate proembryogenic masses that were smaller than 530 microm. Dispersed callus of three cultivars, Kardinal, Classy, and Tineke, produced 61-135 cotyledonary-stage embryos/100 mg fresh weight (FW) as compared to intact callus that had not been dispersed, which produced only zero to three cotyledonary-stage embryos/100 mg FW. Over 500 cotyledonary-stage embryos/100 mg FW callus developed from proembryogenic masses of Kardinal, Classy, and Tineke following 2 months of culture on solidified Murashige and Skoog's basal salts medium supplemented with 0.25% activated charcoal. Cotyledonary-stage embryos of Classy that developed from both dispersed callus and fractionated cells of various sizes showed a significantly higher conversion frequency to plants (28%) than cotyledonary-stage embryos isolated from intact callus (9%). The highest conversion frequencies for Kardinal (50-58%) occurred from cotyledonary-stage embryos that developed from dispersed callus and from the fraction of cells smaller than 850 microm.

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Regeneration through somatic embryogenesis from petal-derived calli of Rosa hybrida L. cv arizona (hybrid tea)

Cited by 33 publications

References 11 publications

An efficient system to produce transgenic plants via cyclic leave-originated secondary somatic embryogenesis in Rosa rugosa

An efficient system to produce transgenic plants via cyclic leave-originated secondary somatic embryogenesis in Rosa rugosa

Organogenesis of vegetative shoots from in vitro cultured flower buds of Mammillaria albicoma (Cactaceae)

Dispersal and size fractionation of embryogenic callus increases the frequency of embryo maturation and conversion in hybrid tea roses

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