Organogenesis of vegetative shoots from in vitro cultured flower buds of Mammillaria albicoma (Cactaceae)

Wyka, Tomasz P.; Hamerska, M.; Wróblewska, M.

doi:10.1007/s11240-006-9128-9

Cited by 15 publications

(13 citation statements)

References 21 publications

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“…(Juárez and Passera, 2002;Estrada et al, 2008); Coryphantha retusa (Ruvalcaba-Ruiz et al, 2010); Turbinicarpus spp. (Dávila et al, 2005) and Mammillaria albicoma (Wyka et al, 2006). In vitro propagation of these species and various cacti have been successfully employed in different culture media (Vidican and Cachita, 2010) but the MS medium (1962) has been the most satisfactory.…”

Section: Inducción De Brotesmentioning

confidence: 99%

“…In callus differentiation in Mammillaria albicoma and Coryphantha retusa the best results were achieved with naphthaleneacetic acid with a concentration range of 2.6 and 26μM (Wyka et al, 2006;Ruvalcaba et al, 2010). The individual action of auxins or the combination with cytokinins and its concentrations determine the success of the organogenic responses observed during various propagation techniques (Quiala et al, 2004).…”

Section: Inducción De Brotesmentioning

confidence: 99%

“…(Dávila et al, 2005) y Mammillaria albicoma (Wyka et al, 2006). En la propagación in vitro de estas especies y diversas cactáceas se han empleado de manera exitosa diferentes medios de cultivo (Vidican y Cachita, 2010) pero el medio MS (1962) ha resultado el más satisfactorio.…”

Section: Calli Proliferation and Shoot Multiplicationunclassified

“…Para el establecimiento de un sistema de propagación in vitro en cactáceas se pueden emplear diversos tejidos u órganos como explantes, cuya elección depende del género y la especie. Se pueden usar brotes terminales de plántulas (Gómez et al, 2006), secciones laterales o verticales de cladodios (Estrada et al, 2002); aréolas simples (Pérez y Dávila, 2002;Ramírez et al, 2007;Quiala et al, 2009);semillas (Dávila et al, 2005;De Madeiros et al, 2006) y brotes florales (Wyka et al, 2006), entre otros, los cuales son cultivados con diferentes reguladores de crecimiento (auxinas, citocininas y giberelinas) de acuerdo con los objetivos de estudios y las respuestas morfogénicas deseadas.…”

Section: Introductionunclassified

“…Terminal shoots of seedlings can be used (Gómez et al, 2006), lateral or vertical sections of cladodes (Estrada et al, 2002); simple areolas (Pérez y Dávila, 2002;Ramírez et al, 2007;Quiala et al, 2009);seeds (Dávila et al, 2005;De Madeiros et al, 2006) and flower buds (Wyka et al, 2006), among others, which are grown with different growth regulators (auxins, cytokinins and gibberellins) in accordance with the study objectives and desired morphogenic responses.…”

Section: Introductionmentioning

confidence: 99%

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Morfogénesis in vitro de Mammillaria plumosa Weber

Téllez-Román

López-Peralta

Hernández-Meneses

et al. 2017

Remexca

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Mammillaria plumosa es una cactácea mexicana altamente apreciada como planta ornamental por su peculiar morfología. La extracción desmedida y el saqueo de sus poblaciones silvestres han forzado su protección y actualmente se encuentra clasificada como una especie en peligro de extinción. Ante esta crítica situación el presente trabajo se planteó como objetivo desarrollar un sistema de propagación in vitro eficiente factible de implementarse como una de las estrategias para la recuperación de la especie. Segmentos de tallos con aréolas se sembraron en medio MS (1962), suplementado con 2,4-D (9, 13.5 y 18 μM) en combinación con cinetina (4.6, 9.3 y 13.9 μM). Todos los tratamientos evaluados indujeron la formación de callos pero la activación de las aréolas para su conversión en brotes solo se obtuvo con 18 μM de 2,4-D y 9.3 μM de cinetina. En la etapa de proliferación los callos continuaron su crecimiento en las mismas concentraciones de reguladores de crecimiento, la diferenciación de brotes se produjo a partir de la activación areolar y la diferenciación de brotes adventicios de novo. Los brotes formaron raíces de forma natural en el proceso pero el enraizamiento se mejoró con el cultivo en medio MS (1962) a la mitad de concentración de sales. En la aclimatación de plantas la tasa de supervivencia fue 85% en sustrato de turba y arena de río. Con este protocolo es posible establecer la micropropagación de Mammillaria plumosa donde se pueden regenerar un promedio 500 plantas en 24 semanas de cultivo, las cuales podrían servir en la restauración de poblaciones silvestres o para su aprovechamiento comercial.

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Section: Inducción De Brotesmentioning

confidence: 99%

Section: Inducción De Brotesmentioning

confidence: 99%

Section: Calli Proliferation and Shoot Multiplicationunclassified

Section: Introductionunclassified

Section: Introductionmentioning

confidence: 99%

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Morfogénesis in vitro de Mammillaria plumosa Weber

Téllez-Román

López-Peralta

Hernández-Meneses

et al. 2017

Remexca

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Embryo rescue and plant regeneration following interspecific crosses in the genus Hylocereus (Cactaceae)

Cisneros

Tel-Zur

2010

Euphytica

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The aim of this study was to develop an efficient methodology to rescue embryos following interspecific crosses in the genus Hylocereus. Crosses between the diploids Hylocereus polyrhizus and H. undatus in both directions were performed. Fertilized ovules carrying embryos at very early pro-embryonic stages were excised from ovaries 5 days after pollination (DAP) and placed on half-strength basal MS medium containing 680 lM glutamine, 0.55 lM a-naphthaleneacetic acid (NAA), 0.45 lM thidiazuron (TDZ) and various concentrations of sucrose. After 30 days in culture, ovules were isolated from the surrounding tissue and transferred to the same fresh medium. Significant differences were found between the main effects (cross and sucrose concentration) in ovule response, i.e., increased ovule size and callus formation. The best responses were obtained in the cross: H. polyrhizus 9 H. undatus; and sucrose concentration of 0.09 M. In terms of embryo conversion, polyembryony and number of regenerated plants, the highest responses were observed on the culture medium supplemented with 0.17 M sucrose in both interspecific crosses. All tested plants were found to be diploid by flow cytometric analyses. Fluorescent amplifiedfragment length polymorphism (fAFLP) confirmed the hybrid origin of the regenerated plants. This study reports on the success of a three-step embryo rescue procedure for Hylocereus species. The procedure developed here provides the means for producing plants from very-early embryo stage, thus expanding the prospects for vine-cactus breeding programs.

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In vitro propagation of purple pitahaya (Hylocereus costaricensis [F.A.C. Weber] Britton & Rose) cv. Cebra

Viñas

Fernández-Brenes

Azofeifa

et al. 2012

In Vitro Cell.Dev.Biol.-Plant

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Limitations to large-scale propagation of purple pitahaya (Hylocereus costaricensis [F.A.C. Weber] Britton & Rose), a potential source of betalains for the food industry, can be overcome through utilization of in vitro culture technologies. In this work, successful in vitro propagation from areoles of adult purple pitahaya plants is reported. Factors affecting culture initiation, bud sprouting and growth, shoot multiplication, rooting, and acclimatization were studied. Best results for culture initiation were obtained from the central region of new joints by disinfection of large (5-7 cm in length) explants that were subsequently divided. Explants were sequentially treated with Extran® alkaline detergent for 10 min, followed by immersion in 70 % (v/v) ethanol for 15-30 s, a mixture of the fungicide Benomyl and the bactericide Agrymicin (2 gl −1 each) for 30 min, and disinfection in sodium hypochlorite (1.0 %w/v) for 15 min. Culture of sectioned individual areoles, without removing thorns, on Murashige and Skoog medium supplemented with 15 or 30 μM N 6 -benzylaminopurine (BAP) for 3 mo with monthly subcultures, followed by transfer to the same medium with reduced BAP (0-2 μM), induced bud sprouting in over 80 % of explants, adequate growth of the shoots, with production of lateral shoots, and spontaneous rooting within 160 d. These plants were successfully acclimatized in vermiculite and peat moss (1:1), or perlite and peat moss (2:1) in the greenhouse, with over 90 % survival rate. One hundred percent of the in vitroderived plants were successfully transferred to the field. Furthermore, these plants showed higher survival rates, larger height and increase in stem diameter than equivalent plants from the same genotype, derived from stem segments (the common clonal propagation system utilized for this species) that were simultaneously planted.

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Organogenesis of vegetative shoots from in vitro cultured flower buds of Mammillaria albicoma (Cactaceae)

Cited by 15 publications

References 21 publications

Morfogénesis in vitro de Mammillaria plumosa Weber

Morfogénesis in vitro de Mammillaria plumosa Weber

Embryo rescue and plant regeneration following interspecific crosses in the genus Hylocereus (Cactaceae)

In vitro propagation of purple pitahaya (Hylocereus costaricensis [F.A.C. Weber] Britton & Rose) cv. Cebra

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