Regeneration of pineapple plants via somatic embryogenesis and organogenesis

Firoozabady, E.; Moy, York

doi:10.1079/ivp2003494

Cited by 44 publications

(54 citation statements)

References 13 publications

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“…The leaf basal region may have improved response to in vitro morphogenesis due to its proximity to the axillary meristem, which contain both meristematic regions and newly formed tissues with high metabolic activity (Firoozabady & Moy, 2004). In agreement to our results, the leaf basal portion of Dendrobium 'Chiengmai Pink' also showed a greater ability to directly form somatic embryos (Chung, Chen, & Chang, 2007).…”

Section: Discussion Plbs Inductionsupporting

confidence: 88%

Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of Cattleya tigrina A. Richard

Almeida

Fraga

Navarro

et al. 2017

Acta Sci. Biol. Sci.

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ABSTRACT. Cattleya tigrina is endemic to the Atlantic forest biome and classified as vulnerable in the Red Book of Brazilian Flora. In vitro techniques comprise valuable tools for the conservation of endangered plant species. The aim of the present study was to evaluate the morphological features, global DNA methylation levels and free polyamines during protocorm-like bodies (PLBs) induction of C. tigrina. Along with that, an efficient protocol for in vitro propagation of this species is proposed. The first evidences of PLBs induction in C. tigrina occurred at seven days in culture, starting from the basal portion of the leaf abaxial surface. A hypomethylation marked the beginning of cell differentiation, followed by an increased global DNA methylation at 35 days in culture, coinciding with a subtle change in the structures morphogenetic development. During PLBs induction, putrescine exhibited higher levels as compared to spermidine and spermine, and apparently presents a major role during the PLBs induction in C. tigrina. Due to the apparent secondary PLBs formation, this protocol can represent a highly efficient method for in vitro propagation of this species.Keywords: orchids, micropropagation, in vitro culture, epigenetics, PLBs.Dinâmica da metilação do DNA global e níveis endógenos de poliaminas durante a indução de estruturas semelhantes a protocormos de Cattleya tigrina A. Richard RESUMO. Cattleya tigrina é uma espécie endêmica do bioma Mata Atlântica e classificada como vulnerável no Livro Vermelho da Flora Brasileira. As técnicas in vitro compreendem ferramentas valiosas a serem empregadas na conservação de espécies de plantas ameaçadas. O objetivo do presente estudo foi avaliar as características morfológicas, os níveis globais de metilação do DNA e as poliaminas livres durante a indução de estruturas semelhantes a protocormos (ESPs). Paralelamente, um protocolo eficiente para a propagação in vitro desta espécie é apresentado. As primeiras evidências de indução de ESPs em C. tigrina foram observadas aos sete dias de cultivo, a partir da porção basal da superfície abaxial da folha. Uma hipometilação foi observada concomitante ao início da diferenciação celular, e um aumento da metilação global do DNA foi encontrada aos 35 dias de cultivo, coincidindo com uma sutil mudança no desenvolvimento morfogenético das estruturas. Durante a indução de ESPs, a putrescina exibiu níveis aumentados em comparação a espermidina e espermina e, aparentemente, apresenta um papel importante durante a indução dessas estruturas em C. tigrina. Devido à aparente formação secundária de ESPs, este protocolo pode representar um método altamente eficiente para a propagação in vitro desta espécie.Palavras-chave: orquideas, micropropagação, cultivo in vitro, epigenética, ESPs.

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Section: Discussion Plbs Inductionsupporting

confidence: 88%

Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of Cattleya tigrina A. Richard

Almeida

Fraga

Navarro

et al. 2017

Acta Sci. Biol. Sci.

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“…Hence, tests were conducted without picloram and additives in addition to plant growth regulators, which showed that the SEs maturation was significantly reduced (data not shown). Firoozabady and Moy (2004) reported picloram as one of embryogenic potentials agent to increase the growth regulators in Ananas comosus. However, picloram regulated the embryogenic stages and produced maximum frequency of SEs and plant germination (Little et al, 2000;Groll et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Effect of picloram, additives and plant growth regulators on somatic embryogenesis of Phyla nodiflora (L.) Greene

Ahmed

Rao

et al. 2011

Braz. arch. biol. technol.

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“…The experimental design was factorial in Completely Randomized Design with 10 replications. The first factor was basal media (MS and Bac) and the second factor was adenine sulphate (AdS) at the rate of 0, 0.05, and 0.1 uM (0, 20, and 40 mg l ) which was used by Firoozabady and Moy (2004). The calli including original explants were placed in the room culture with 25±2 o C, in the dark condition for 3 weeks.…”

Section: Indirect Somatic Embryogenesis Regenerationmentioning

confidence: 99%

“…However, this method should be applied to provide a large number of seedlings of important new cultivars resulted from hybridization, selection, mutation, and genetic engineering (Firoozabady and Moy, 2004;Nursandi et al, 2005). In practice, micropropagation is used for the establishment of multiplication blocks which then provide conventional planting material for larger production blocks.…”

Section: Introductionmentioning

confidence: 99%

Indirect Organogenesis and Somatic Embryogenesis of Pineapple Induced by Dichlorophenoxy Acetic Acid

et al. 2016

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This research aimed to study the effect of 2,4-D, AdS, and basal media to the regeneration of pineapple through indirect organogenesis and somatic embryogenesis, and to study the complete event of somatic embryogenesis. Callus formation was induced by 21, 41, and 62 μM 2,4-D with addition of 9 μM TDZ. The non embryogenic calli were transferred onto 4.65 μM Kn containing medium. Embryogenic callus formation was induced on MS or Bac basal media consisted of N-organic compounds with addition of AdS (0, 0.05 and 0.1 μM). The embryogenic calli were regenerated on modified MS medium with addition of 0.9 μM IBA, 1.1 μM BA, 0.09 μM GA3 or MS medium supplemented with 0.018 mM BA. The result proved that the single auxin of 2,4-D was not enough to induce embryogenic cells. Therefore the non embryogenic calli were regenerated through organogenesis. The 21 μM 2,4-D yielded high level of callus formation (80%), higher fresh weight (0.2 g/explant) and higher number of shoot (25 shoots/explant in two months). Embryogenic calli were produced on N-organic compounds enriched media. The regeneration medium significantly affected the level of browning, where the MS medium with addition of 0.018 mM BA yielded lower level of browning. There was an interaction of embryogenic callus induction medium and regeneration medium to the number of mature somatic embryos. The embryogenic callus induction on MS medium enriched with N-organic compounds and 0.05 μM AdS followed by the regeneration of somatic embryos on MS medium with addition of 0.018 mM BA was the best treatment which yielded 17 mature somatic embryos/explant.

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Regeneration of pineapple plants via somatic embryogenesis and organogenesis

Cited by 44 publications

References 13 publications

<b>Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of <i>Cattleya tigrina</i> A. Richard

<b>Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of <i>Cattleya tigrina</i> A. Richard

Effect of picloram, additives and plant growth regulators on somatic embryogenesis of Phyla nodiflora (L.) Greene

Indirect Organogenesis and Somatic Embryogenesis of Pineapple Induced by Dichlorophenoxy Acetic Acid

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