Effect of picloram, additives and plant growth regulators on somatic embryogenesis of Phyla nodiflora (L.) Greene

Ahmed, Abdul Bakrudeen Ali; Rao, A. S.; Rao, M. S.; Taha, Rosna Mat

doi:10.1590/s1516-89132011000100002

Cited by 22 publications

(16 citation statements)

References 26 publications

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“…In addition, it was observed that IAA and IBA drastically reduced the callus biomass resulting in WFC natured calli all the media. These results are well authenticated with the previous reports of Ahmed et al (2011), who demonstrated that NAA and 2,4-D were suitable in the callus induction of P. nodiflora. P. nodiflora callus growth curve was sigmoid type and four growth phases can be distinguished in different days (15, 25, 35, 45 and 55 days).…”

Section: Callus Induction and Growth Curve Analysissupporting

confidence: 91%

Optimized conditions for callus induction, plant regeneration and alkaloids accumulation in stem and shoot tip explants of Phyla nodiflora

Ahmed¹,

Pallela²,

Rao³

et al. 1970

Span. j. agric. res.

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The present study describes callus induction and the subsequent plant regeneration with alkaloids accumulation in stem and shoot tip explants of Phyla nodiflora. Both explants were cultured on different media (MS, B5, SH and WPM) for callus induction. Stem explants showed better callus biomass (dry weight) than shoot tip explants with green compact callus when cultured on MS medium containing 1.5 mg L -1 α-naphthalene acetic acid. Shoots were regenerated from the callus on MS medium with 1.5 mg L -1 α-naphthalene acetic acid and 1.0 mg L -1 benzyl adenine. The rooting of all regenerated shoots was successfully performed on half-strength MS medium with 1.0 mg L -1 indole-3-butyric acid. The plantlets were acclimatized and established in soil (90%) and exhibited morphological characteristics similar to those of the mother plant. In addition, the alkaloids content was higher in regenerated callus than intact stem and shoot tip explants, which were analyzed by a gravimetric method, TLC (thin layer chromatography) and HPTLC (high performance thin layer chromatography). The proposed method could effectively be applied for the conservation and clonal propagation to meet the pharmaceutical demands of this medicinally important species.Additional key words: callus biomass; gravimetric method; growth curve; HPTLC; rooting; TLC. ResumenCondiciones optimizadas para inducción de callos, regeneración de plantas y acumulación de alcaloides en explantes de tallos y brotes de Phyla nodiflora El presente estudio describe la inducción de callos y la posterior regeneración de plantas con la acumulación de alcaloides en los explantes de tallos y brotes de Phyla nodiflora. Para la inducción de callos, ambos explantes fueron cultivados en diferentes medios (MS, B5, SH y WPM). Los explantes de tallos tuvieron mejor biomasa (peso seco) de callo que explantes de ápices de naturaleza verde compacta cuando se cultivaron en un medio MS con 1,5 mg L -1 de ácido α-naftalén acético. Los brotes fueron regenerados a partir de los callos en medio MS con 1,5 mg L -1 de ácido α-naftalén acético y 1,0 mg L -1 de benciladenina. El enraizamiento de todos los brotes regenerados se realizó con éxito en un medio ½ MS con 1,0 mg L -1 de ácido indol-3-butírico. Las plántulas fueron aclimatadas y establecidas en suelo (en un 90%) y mostraron características morfológicas similares a las de la planta madre. Además, el contenido de alcaloides fue mayor en los callos regenerados que en los explantes de tallo y brotes intactos, que fueron analizados por un método gravimétrico, TLC y HPTLC. El método propuesto se podría aplicar con eficacia para la conservación y propagación clonal, a fin de satisfacer la demanda farmacéutica de esta especie medicinalmente importante.

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Section: Callus Induction and Growth Curve Analysissupporting

confidence: 91%

Optimized conditions for callus induction, plant regeneration and alkaloids accumulation in stem and shoot tip explants of Phyla nodiflora

Ahmed¹,

Pallela²,

Rao³

et al. 1970

Span. j. agric. res.

View full text Add to dashboard Cite

show abstract

“…Figure 4 also showed that picloram-derived cultures had the lowest similarity (0.34). Picloram is an auxinic herbicide belong to the pyridine group and commonly used at low concentration as an inducer for callus formation during somatic embryogenesis regeneration (Firoozabady & Moy 2004;Ahmed et al et al 2011;Noormi 2012). Beside regenerated through callus formation phase, it needs a longer time to conduct somatic embryogenesis, so that the probability of genetic changes would increase.…”

Section: Regeneration Methods Affects the Somaclonal Variation Of Pinementioning

confidence: 99%

RAPD Analysis to Detect Somaclonal Variation of Pineapple In Vitro Cultures during Micropropagation

Roostika

Khumaida²,

Ardie³

2016

BIOTROPIA

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Plant off-type becomes a concern in pineapple micropropagation. Reliable methods are needed to detect and to reduce plant off-type. This research was conducted to confirm the occurrence of somaclonal variation during micropropagation of pineapple clone Simadu. The culture period (long-and short-period) and the regeneration methods (direct organogenesis, indirect organogenesis and somatic embryogenesis) were studied to know their contribution in bringing out somaclonal variation. RAPD analysis using 10 primers was performed to confirm genetic variation. The result showed that RAPD assay could be applied as early detection of somaclonal variation of pineapple, where OPA primers were better to be used than OPJ primers. The phenotypic variation occurred in the four year-old pineapple plants and plantlets were due to genetic variation. It has proved that the long-period of cultures is the main contributor of somaclonal variation, while the regeneration method and plant growth regulator could also induce genetic variation. The new cultures showed higher level of similarity. Therefore, it needs a correct strategy to apply micropropagation method of pineapple to minimize plant off-type. It was recommended to avoid the use of long-period culture as mother stock and to apply direct organogenesis method for micropropagation rather than indirect organogenesis and somatic embryogenesis.

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“…Plants also produce certain chemicals to overcome abiotic stresses. In this aspects plant tissue culture developed callus influenced by medium, explants, plant growth regulators, color lights, temperature, photoperiod and carbon sources are helpful to produce valuable secondary metabolites compounds in many studies [3,4]. Growing a plant outside its natural environment under ideal conditions may therefore, result in being unable to produce the desired bioactive substances, hence the need for prior evaluation.…”

Section: Application Areamentioning

confidence: 99%

“…Environmental factors on the other hand are the culture conditions under which explants are maintained. The environmental factors involved include the temperature and illumination of the culture room, agitation process and incubation period of the cultures [4]. For the initiation of callus culture, the following factors are important-: the origin of explants used for the establishment of callus culture, the cellular/tissue differentiation status, external plant growth regulators, culture media and culture conditions [46].…”

Section: Organogenesismentioning

confidence: 99%

In vitro Regeneration, Acclimatization and Antimicrobial Studies of Selected Ornamental Plants

Ahmed¹,

Mohajer²,

Elnaiem³

et al. 2012

Plant Science

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Effect of picloram, additives and plant growth regulators on somatic embryogenesis of Phyla nodiflora (L.) Greene

Abstract: The present study describes the plant regeneration via somatic embryogenesis in suspension culture derived from the leaf and stem explants of Phyla nodiflora.

Cited by 22 publications

References 26 publications

Optimized conditions for callus induction, plant regeneration and alkaloids accumulation in stem and shoot tip explants of Phyla nodiflora

Optimized conditions for callus induction, plant regeneration and alkaloids accumulation in stem and shoot tip explants of Phyla nodiflora

RAPD Analysis to Detect Somaclonal Variation of Pineapple In Vitro Cultures during Micropropagation

In vitro Regeneration, Acclimatization and Antimicrobial Studies of Selected Ornamental Plants

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