Regeneration of Pancreatic B Cells of Type 1 Diabetic Mouse by Stem Cell Transplatation

Phuc, Pham Van; Truc, Pham Le Buu; Thuy, Duong Thanh; Nhung, Truong Hai; Chung, Đoàn Chính; Toan, Nguyen Khac; Phuc, Ma Kien; Ngoc, Phan Kim

doi:10.1007/978-3-642-12020-6_40

Cited by 2 publications

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study we induced diabetes mellitus by alloxan an diabetic agent by injecting the mice with 70 mg/kg body weight and all the mice were found to be diabetic after 72 hours. this result is similar to that recorded by (9,17) who use the same diabetic agent other authors use another diabetic agent Streptozotocin and obtained the same result by Phuc et al (18) and we can detect induce the diabetes in the mice by measuring the blood sugar level by using strips.…”

Section: Induction Of Diabetes Mellitussupporting

confidence: 91%

Protective effect of placental mesenchymal stem cells on histological changes of pancreas experimentally induced by alloxane in mice

Ismail¹,

Al-Sabawy²,

Jumaa³

2020

IJVS

View full text Add to dashboard Cite

In an attempt to find a treatment for diabetes by Placental mesenchymal stem cells, we induced diabetes in albino mice by injected them with the diabetic agent Alloxan intra peritoneal cavity dose 70 mg / kg of body weight for the group 1 and 2, then injection of the diabetic mice with Placental mesenchymal stem cells in the peritoneal cavity for the group 2 then the glucose levels in the blood of these mice were measured by using a GLUCOTREND 2, while group 3 was control injected with normal saline only. Pancreas tissue where tested by studying histopathological effect of Alloxan, the endocrine pancreas histologically showed decreased in the size and number of Langerhans islets with vacuolar degeneration and necrosis of almost all cells in the atrophied islets. Also, there was congestion of blood vessels in the interlobular space and there was distortion of exocrine pancreas. There was vacuolar degeneration and necrosis of the epithelial cells of the acini, also there was atrophy of acini which lead to edema and dilatation of interacini space. Moreover, there was thickening in the wall of blood vessels, and there was thrombus in some blood vessels. In addition to the infiltration of inflammatory cells in the interlobular septa. Compared with the section of pancreas of control group showed the normal structure of islets notice the normal cells shape surrounding by exocrine pancreas. Then the pancreas studied after injection of Placental mesenchymal stem cells, while there was regeneration of islets and acini which seem to be normal comparing with control group, there was improvement of normal histological appearance of mice treated with alloxan and then injected with placental mesenchymal stem cells. The results of the diabetic mice which treated with placental mesenchymal stem cells demonstrated present of significant differences (P≤0.05). Our conclusion of this study revealed that placental mesenchymal stem cells have a protective role against the histological changes of pancreas induced by alloxan.

show abstract

Section: Induction Of Diabetes Mellitussupporting

confidence: 91%

Protective effect of placental mesenchymal stem cells on histological changes of pancreas experimentally induced by alloxane in mice

Ismail¹,

Al-Sabawy²,

Jumaa³

2020

IJVS

View full text Add to dashboard Cite

show abstract

“…Liczne badania były poświęcone możliwości pozyskiwania komórek beta z komórek macierzystych szpiku, krwi pępowinowej i tkanki mezenchymalnej trzustki [55][56][57]. Zespołowi Pagliuca i wsp.…”

Section: Neogenezaunclassified

“…Obiecujące wyniki uzyskali Van Phuc i wsp. [55] obserwując różnico-wanie się komórek beta z mezenchymalnych komórek macierzystych uzyskanych z ludzkiej krwi pępowinowej. Według nich otrzymano lepsze efekty produkcji insuliny i lepsze efekty odpowiedzi na poziomy glukozy w porównaniu do komórek pobranych ze szpiku czy komórek macierzystych wywodzących się z tkanki trzustkowej.…”

Section: Neogenezaunclassified

Restrain of beta-cell destruction and stimulation of beta-cell regeneration in type 1 diabetes

Szewczyk¹,

Bury²,

Pierpoint³

2015

Pediatr. Endocrinol.

View full text Add to dashboard Cite

The contemporary date on the microenvironment of pancreatic beta cells have been presented. Attention was drawn to successful attempts to inhibition of beta cells destruction after the diagnosis of type 1 diabetes. The attractive attempts of stimulation of beta cells regeneration by their proliferation, neogenesis from other non-endocrine elements or conversion of alfa to beta cells has been traced, also the role of the autonomic nervous system in these processes has been underlined. Pediatr. Endocrinol. 2015.14.3.52.35-40. © Copyright by PTEiDD 2015 Słowa kluczowe cukrzyca typu 1, mikro-środowisko komórek beta, hamowanie destrukcji komórek beta, regeneracja komórek beta Streszczenie W pracy zaprezentowano współczesne dane dotyczące funkcjonowania mikrośrodowiska komórek beta trzustki. Zwrócono uwagę na udane próby hamowania destrukcji komórek beta po rozpoznaniu cukrzycy typu 1. Prześledzono atrakcyjne próby mające na celu pobudzenie regeneracji komórek beta poprzez ich proliferację, neogenezę z innych elementów nieendokrynnych czy konwersję komórek alfa do beta. Podkreślono także rolę autonomicznego układu nerwowego w tych procesach.

show abstract

Regeneration of Pancreatic B Cells of Type 1 Diabetic Mouse by Stem Cell Transplatation

Cited by 2 publications

References 10 publications

Protective effect of placental mesenchymal stem cells on histological changes of pancreas experimentally induced by alloxane in mice

Protective effect of placental mesenchymal stem cells on histological changes of pancreas experimentally induced by alloxane in mice

Restrain of beta-cell destruction and stimulation of beta-cell regeneration in type 1 diabetes

Contact Info

Product

Resources

About