Regeneration from Phylloclade Explants and Callus Cultures of Schlumbergera and Rhipsalidopsis

Sriskandarajah, Sridevy; Serek, Margrethe

doi:10.1023/b:ticu.0000020398.11717.88

Cited by 17 publications

(20 citation statements)

References 10 publications

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“…Sriskandarajah and Serek (2004) reported that accumulation of cytokinin-like substances due to repeated culture in media containing cytokinin may have contributed to balance out the high endogenous levels of auxins, thus improving adventitious shoot formation in phylloclade explants of cultivar CB4 of Rhipsalidopsis. Chen and Chang (2001) found that embryo formation was retarded on leaf explants of Oncidium 'Grower Ramsey' by the four auxins tested (IAA, IBA, NAA and 2,4-D) whereas the highest percentage of embryo formation was recorded using media containing TDZ, 2iP and kinetin.…”

Section: Discussionmentioning

confidence: 98%

“…In vitro propagation is a potential alternative for production of these plants (Hubstenberger et al, 1992). While axillary and adventitious shoots in Schlumbergera and Rhipsalidopsis have been produced in vitro from phylloclade explants and callus cultures (Sriskandarajah and Serek, 2004) and from shoot tips (Johnson et al, 1976), the frequency at which these shoots are produced is still slow and unsatisfactory.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Plant regeneration via somatic embryogenesis in Schlumbergera truncata

Al-Ramamneh¹,

Sriskandarajah²,

Serek

2006

Plant Cell Tiss Organ Cult

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Somatic embryogenesis was induced from phylloclade explants of Schlumbergera truncata cv. Russian Dancer. Callus developed on phylloclade explants and sub-cultured over a period of 16 months on MS medium containing mainly cytokinins was superior for the induction of somatic embryos compared to callus grown for a shorter time in the establishment medium. Sub-culture of callus grown in SH-or MSbased liquid media supplemented with 7.0 lM kinetin and transferred onto solid MS-based medium with either 0.45 lM 2,4-D or without hormones resulted in the differentiation into somatic embryos. SH-based medium proved better than MS-based medium when used as the first medium for the induction of somatic embryogenesis. However, somatic embryogenesis, contrary to adventitious shoot formation, was reduced when 2,4-D was included in the MS-based medium used for final transfer compared to the medium without growth regulators, indicating that a critical hormonal balance was reached. Somatic embryos developed root and shoot poles when grown on G medium. On this medium approximately 70% germination was recorded in the embryos that were differentiated earlier from the callus that was grown for a longer time in the establishment medium. This callus was grown on either SH-or MS-based medium supplemented with 7.0 lM kinetin, and then transferred after 30 days (from SH medium) onto MS medium without hormones or after 40 days (from MS medium) onto MS medium with 0.45 lM 2,4-D. Furthermore, plants from somatic embryos were successfully potted in soil and showed further growth and formation of a second set of phylloclades (secondary phylloclades). Histological studies showed that somatic embryos had no detectable connection with the mother explants and that advanced stages of somatic embryos had a contained vascular system. In addition to the normal dicotyledonous embryos, anomalous embryos with multiple cotyledons and vase-like embryos were observed. Secondary embryos were also recorded in this study.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Plant regeneration via somatic embryogenesis in Schlumbergera truncata

Al-Ramamneh¹,

Sriskandarajah²,

Serek

2006

Plant Cell Tiss Organ Cult

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“…As espécies da família Cactaceae possuem caracterís-ticas especializadas para sobreviverem em ambientes ári-dos, tais como, a presença de espinhos, de cera, de parênquima aquífero, de sistema radicular fasciculado e de Mecanismo Ácido das Crassuláceas (CAM), que reduzem a perda de água (Sriskandarajah & Serek, 2004;Arruda et al, 2005;Khalafalla et al, 2007). Essas características podem ter favorecido a independência da etapa de rustificação e os valores altos para a sobrevivência nas condições ex vitro.…”

Section: Efeito Do Substrato Na Aclimatização Dos Brotos Obtidos In Vunclassified

“…Malda et al (1999) A formação de raízes in vitro e ex vitro em M. glaucescens, sem a necessidade da utilização de regulador vegetal, indica que a concentração dos hormônios endógenos é suficiente para promover a formação das raízes dos brotos nessa espécie. Os cactos, em geral, produzem um excesso de auxina sob condições de cultivo in vitro, que pode favorecer a formação de raízes em meio livre de regulador vegetal (Hubstenberger et al, 1992;Sriskandarajah & Serek, 2004).…”

Section: Efeito De Diferentes Períodos De Enraizamento Na Aclimatizaçunclassified

Influência do substrato e do enraizamento na aclimatização de Melocactus glaucescens Buining & Brederoo propagados in vitro

2010

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Effect of substrate and rooting on acclimatization of in vitro propagated Melocactus glaucescens Buining & BrederooMelocactus glaucescens (Cactaceae) is endemic to Bahia and is included in the IUCN and MMA lists as endangered species. Transference of in vitro cultures to ex vitro environment is critical and can become limiting for the production of micropropagated scions. The main objective of this work was to examine the effect of different substrates and rooting during acclimatization of Melocactus glaucescens. The plants were in vitro propagated and kept in 100 % light, on daily cycles for 75 days. The results suggested that the best substrate should consist of 50 % soil and 50 % sand; plantlets should be at least 5mm in diameter and height before being transferred; and that both steps in vitro rooting and harding could be eliminated from micropropagation of M. glaucescens. Further studies are still needed on desiccation time of shoots above 5 mm so that the step of in vitro rooting can be completely eliminated.

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“…Pairs of areoles were dissected from the cladodes and were used as explants. Sriskandarajah and Serek (2004) also started in vitro cultures using phylloclade explants of Schlumberguera and Rhipsalidopsis from plants grown in the greenhouse. Due to the contamination issue frequently faced during the establishment of in vitro cultures of cacti species, many investigators have started from in vitro aseptic seedlings or other secondary explants axcissed from in vitro cultures.…”

Section: In Vitro Culture Establishmentmentioning

confidence: 99%

Tissue culture of ornamental cacti

Pérez-Molphe-Balch

Santos-Díaz

Ramírez-Malagón

et al. 2015

Sci. agric. (Piracicaba, Braz.)

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Cacti species are plants that are well adapted to growing in arid and semiarid regions where the main problem is water availability. Cacti have developed a series of adaptations to cope with water scarcity, such as reduced leaf surface via morphological modifications including spines, cereous cuticles, extended root systems and stem tissue modifications to increase water storage, and crassulacean acid metabolism to reduce transpiration and water loss. Furthermore, seeds of these plants very often exhibit dormancy, a phenomenon that helps to prevent germination when the availability of water is reduced. In general, cactus species exhibit a low growth rate that makes their rapid propagation difficult. Cacti are much appreciated as ornamental plants due to their great variety and diversity of forms and their beautiful short-life flowers; however, due to difficulties in propagating them rapidly to meet market demand, they are very often over-collected in their natural habitats, which leads to numerous species being threatened, endangered or becoming extinct. Therefore, plant tissue culture techniques may facilitate their propagation over a shorter time period than conventional techniques used for commercial purposes; or may help to recover populations of endangered or threatened species for their re-introduction in the wild; or may also be of value to the preservation and conservation of the genetic resources of this important family. Herein we present the state-of-the-art of tissue culture techniques used for ornamental cacti and selected suggestions for solving a number of the problems faced by members of the Cactaceae family.

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Regeneration from Phylloclade Explants and Callus Cultures of Schlumbergera and Rhipsalidopsis

Cited by 17 publications

References 10 publications

Plant regeneration via somatic embryogenesis in Schlumbergera truncata

Plant regeneration via somatic embryogenesis in Schlumbergera truncata

Influência do substrato e do enraizamento na aclimatização de Melocactus glaucescens Buining & Brederoo propagados in vitro

Tissue culture of ornamental cacti

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