Regeneration and Transformation of a Roadside Tree Pittosporum tobira A.

Kondo, Komei; Takahashi, Misa; Morikawa, Hiromichi

doi:10.5511/plantbiotechnology.19.135

Cited by 13 publications

(5 citation statements)

References 15 publications

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“…An in vitro aseptic culture of Ficus thunbergii, an evergreen creeper, was grown for three months as reported in [33]. Seeds of roadside shrubs Rhaphiolepis umbellata [34] and Pittosporum tobira [33] were sterilised and cultivated on a woody plant medium for three to four months as reported in [35]. Seeds of roadside trees Eucalyptus viminalis and Eucalyptus globulus were sterilised and cultivated for two months [10].…”

Section: Plant Materialsmentioning

confidence: 99%

“…Conversion of nitrogen dioxide (NO 2 ) to nitrous oxide (N 2 O) by the wild type (WT) and transgenic (line 271) tobacco This transgenic tobacco practically lacks nitrite reductase activity[39]. The tobacco plants were aseptically cultured on B medium[35] for seven weeks, and fumigated with 1 or 4 ppm of 15 NO 2 for five days, after which they were transferred onto a B medium in gas-sealed pots without the addition of NO 2 . Then the cultivation was continued for three days.…”

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confidence: 99%

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Conversion of the Nitrate Nitrogen and Nitrogen Dioxide to Nitrous Oxides in Plants

Hakata

Takahashi

Zumft

et al. 2003

Acta Biotechnologica

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Tobacco and wheat are known to emit nitrous oxide (N 2 O). Provided that N 2 O is a greenhouse gas about 300 times worse than carbon dioxide in its potential for global warming, it is an intriguing and important subject, whether this activity is commonly present in other plants. In this study, 17 plant taxa that had been cultured aseptically were fed with 15 N-labelled nitrate for one week (feeding period). The plant taxa were then transferred to a medium with non-labelled nitrate in gas-sealed pots and cultured for another week (emission period). The amount of labelled N 2 O emitted from the plants during the emission period was determined. This value reflects only a part of the capability of plants to convert nitrate to N 2 O, because the amount of N 2 O determined here does not correspond to the total N 2 O emission from plants, but to only an 15 N-labelled N 2 O fraction emitted during the emission period from nitrate taken up into the plants during the feeding period. It was discovered that all of the 17 plant taxa analysed, except for Eucalyptus viminalis, showed emission of 15 N 2 O. The emission ranged from 0.45 ± 0.20 ng N 2 O/g fresh weight (kenaf) to -0.012 ± 0.12 ng N 2 O/g fresh weight (Eucalyptus viminalis). This activity of converting nitrate to N 2 O is obviously common in plants with some exceptions. There was more than a 58-fold variation between the highest (Hibiscus cannabinus) and the second lowest (Nicotiana tabacum) capability to convert nitrate to N 2 O among the 17 plant taxa. The present result indicates that the potential of plants to convert nitrate to N 2 O highly varies among the plant species as in the case of the assimilation of nitrogen dioxide (N 2 O) where more than a 600-fold variation was observed among 217 plant taxa. A negative correlation was obtained for N 2 O emission and NO 2 assimilation (r = 0.72).This is the first report where there was a competitive interaction between the assimilation and dissimilation activities in plants. It was also found that wild type and transgenic tobacco are able to convert NO 2 to N 2 O when they were fumigated with 15 N-labelled NO 2 . However since the N 2 O emission values observed in this study were approximately three orders of magnitude smaller than those observed with wheat leaves, the evaluation of the environmental significance of the N 2 O emission by plants must await quantitative analysis using the present system to figure out the full N 2 O emission capability of plants.

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Section: Plant Materialsmentioning

confidence: 99%

mentioning

confidence: 99%

Conversion of the Nitrate Nitrogen and Nitrogen Dioxide to Nitrous Oxides in Plants

Hakata

Takahashi

Zumft

et al. 2003

Acta Biotechnologica

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“…In vitro seed germination followed by hypocotyl sectioning to raise plants has been documented in P . tobira [ 9 ]…”

Section: Introductionmentioning

confidence: 99%

Genetic Homogeneity Revealed Using SCoT, ISSR and RAPD Markers in Micropropagated Pittosporum eriocarpum Royle- An Endemic and Endangered Medicinal Plant

et al. 2016

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Pittosporum eriocarpum Royle, a medicinally important taxon, is endemic to Uttarakhand region of Himalaya. It has become endangered due to over-collection and the loss of habitats. As raising plants through seeds in this plant is problematic, a reliable protocol for micropropagation using nodal explants has been developed. High shoot regeneration (95%) occurred in MS medium augmented with BA 0.4mg/l in combination IBA 0.6mg/l. In vitro regenerated shoots were rooted in MS medium supplemented with three auxins, of which 0.6 mg/l indole butyric acid proved to be the best for rooting (90%) with maximum number of roots per shoot. Thereafter, rooted plants were hardened and nearly 73% of rooted shoots were successfully acclimatized and established in the field. Start codon targeted (SCoT), inter simple sequence repeats (ISSR) and random amplified polymorphic DNA (RAPD) markers were used to validate the genetic homogeneity amongst nine in vitro raised plantlets with mother plant. DNA fingerprints of in vitro regenerated plantlets displayed monomorphic bands similar to mother plant, indicating homogeneity among the micropropagated plants with donor mother plant. The similarity values were calculated based on SCoT, ISSR and RAPD profiles which ranged from 0.89 to 1.00, 0.91 to 1.00 and 0.95 to 1.00 respectively. The dendrograms generated through Unweighted Pair Group Method with arithmetic mean (UPGMA) analysis revealed 97% similarity amongst micropropagated plants with donor mother plant, thus confirming genetic homogeneity of micropropagated clones. This is the first report on micropropagation and genetic homogeneity assessment of P. eriocarpum. The protocol would be useful for the conservation and large scale production of P. eriocarpum to meet the demand for medicinal formulations and also for the re-introduction of in vitro grown plants in the suitable natural habitats to restore the populations.

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“…Herbicide Basta is a commercial formulation of glufosinate, which is the ammonium salt of PPT (Fromm et al, 1990). Meanwhile, bialaphos is a tripeptide herbicide produced by Streptomyces hygroscopius consisting of two L-alanine molecules and one L-glutamic acid analog called PPT (Ogawa et al, 1973;Kondo et al, 2002). To date, no study evaluating the use of bialaphos and glufosinate ammonium as selection agent for oil palm has been reported.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Effectiveness of Basta, Bialaphos and Glufosinate Ammonium for Selecting Transformed Oil Palm Tissues

Nurfahisza¹

2016

JOPR

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Regeneration and Transformation of a Roadside Tree Pittosporum tobira A.

Cited by 13 publications

References 15 publications

Conversion of the Nitrate Nitrogen and Nitrogen Dioxide to Nitrous Oxides in Plants

Conversion of the Nitrate Nitrogen and Nitrogen Dioxide to Nitrous Oxides in Plants

Genetic Homogeneity Revealed Using SCoT, ISSR and RAPD Markers in Micropropagated Pittosporum eriocarpum Royle- An Endemic and Endangered Medicinal Plant

Comparison of the Effectiveness of Basta, Bialaphos and Glufosinate Ammonium for Selecting Transformed Oil Palm Tissues

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