Comparison of the Effectiveness of Basta, Bialaphos and Glufosinate Ammonium for Selecting Transformed Oil Palm Tissues

Abstract

One of the important requirements for producing transgenic plants is the ability to

Progress in Tissue Culture and Genetic Transformation of Oil Palm: An Overview

“…To date, researchers have used Basta (50mg/L) as an effective selection agent for oil palm genetic transformation experiments. However, recent reports [68,69] demonstrated the successful use of calli suspension cultures for the production of transgenic oil palms, and this can be recovered on less than 50 mg/L of Basta. Data have shown that concentrations of 10 and 20 mg/L of Basta are required for the growth suppression of embryogenic calli and embryoids (suspension cultures), respectively.…”

“…Data have shown that concentrations of 10 and 20 mg/L of Basta are required for the growth suppression of embryogenic calli and embryoids (suspension cultures), respectively. Nurfahisza et al 2016 [69] also published an article on the effective use of other selection agents such as glufosinate-ammonium and bialaphos at 3 mg/L of the MIC for growth suppression of embryogenic calli.…”

Minimal Inhibitory Concentration of Hygromycin for Selecting Transformed Oil Palm Embryogenic Calli

“…Prior to the transformation of the desired genes, preliminary experiments are often conducted to determine minimal inhibitory concentration (MIC) of selection agent required for efficient selection under established culture condition. For oil palm, a number of MIC studies have been done using different types of selection agents such as kanamycin, geneticin G-418, neomycin, hygromycin, herbicide Basta, phosphomannose isomerase, DOG R 1, bialaphos and glufosinate ammonium Bahariah et al, 2012;Masli et al, 2012;Nurfahisza et al, 2016). Based on the MIC result, the putatively transformed tissues will be selected and regenerated on the specific concentration of the desired selection agent.…”