Refractive index distributions in dehydrated cells of human oral cavity epithelium

Белашов, А. В.; Жихорева, А.А.; Bespalov, V. G.; Novik, V. I.; Zhilinskaya, N. T.; Семенова, И. В.; Vasyutinskiǐ, O. S.

doi:10.1364/josab.34.002538

Cited by 22 publications

(7 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For all reconstructions the borders of the cell and its inner structure with nucleus are clearly seen both in amplitudes and phases. Phase distribution maps show that phase delay in a region of cell nucleus is significantly higher than in other parts of the cell, that corresponds to higher refractive index of the nucleus, knowing that thickness of the cell changes slightly [10]. The reconstruction results for amplitudes for all exposure times are similar, but for phases there are differences between t ex p = 1 ms and others cases.…”

Section: Resultsmentioning

confidence: 82%

See 1 more Smart Citation

Super-resolution microscopy for biological specimens: lensless phase retrieval in noisy conditions

Shevkunov

Katkovnik

Petrov

et al. 2018

Biomed. Opt. Express

View full text Add to dashboard Cite

The paper is devoted to a computational super-resolution microscopy. A complexvalued wavefront of a transparent biological cellular specimen is restored from multiple intensity diffraction patterns registered with noise. For this problem, the recently developed lensless super-resolution phase retrieval algorithm [Optica, 4(7), 786 (2017)] is modified and tuned. This algorithm is based on a random phase coding of the wavefront and on a sparse complex-domain approximation of the specimen. It is demonstrated in experiments, that the reliable phase and amplitude imaging of the specimen is achieved for the low signal-to-noise ratio provided a low dynamic range of observations. The filterings in the observation domain and specimen variables are specific features of the applied algorithm. If these filterings are omitted the algorithm becomes a super-resolution version of the standard iterative phase retrieval algorithms. In comparison with this simplified algorithm with no filterings, our algorithm shows a valuable improvement in imaging with much smaller number of observations and shorter exposure time. In this way, presented algorithm demonstrates ability to work in a low radiation photon-limited mode.

show abstract

Section: Resultsmentioning

confidence: 82%

“…One of the DH's advantages is an ability to reconstruct the object phase, which brings information about a thickness and refractive index of the object [10]. Estimation of these parameters opens great possibility for science in investigation of live creatures.…”

Section: Introductionmentioning

confidence: 99%

Super-resolution microscopy for biological specimens: lensless phase retrieval in noisy conditions

Shevkunov

Katkovnik

Petrov

et al. 2018

Biomed. Opt. Express

View full text Add to dashboard Cite

show abstract

“…Numerical processing of holograms comprised several steps, namely, reconstruction by means of the least square minimization algorithm [34,35], phase unwrapping [36] and cells segmentation. The phase image quality was improved by coherent noise reduction procedure based on the recording of a series of holograms with small lateral shifts of the specimen within the field of view and coherent noise elimination [37]. As a result spatial distributions of integral phase shift induced by the specimens were obtained.…”

Section: Experimental Methodologymentioning

confidence: 99%

Morphological changes in the ovarian carcinoma cells of Wistar rats induced by chemotherapy with cisplatin and dioxadet

Жихорева

Белашов

Беспалов

et al. 2018

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

The development of new express methods for the analysis of the efficacy of anticancer therapy on the cellular level is highly desirable for the analysis of chemotherapeutic agent performance. In this paper we suggest the use of parameters of cell morphology determined by holographic microscopy and tomography for the effective label free quantitative analysis of cell viability under antitumor chemotherapy and thus of cytostatic agent efficacy. As shown, measured phase shifts and cell morphology change dramatically as a result of chemotherapy and depend strongly on the cell type and agent applied. Experimentally, a comparative analysis of the antitumor efficacy of the two cytostatics, cisplatin and dioxadet, that are commonly used for chemotherapy of disseminated ovarian carcinoma has been performed. The experiments were carried out on the Wistar rat model. An essential difference in the morphology of cells, both normal (erythrocytes) and cancerous, present in ascitic fluid taken from the non-treated group of rats and the groups treated with either dioxadet or cisplatin, has been observed. The results obtained can be interpreted as an indication of the antitumor performance of both cytostatics at the cellular level and as a demonstration of the higher efficacy of therapy with dioxadet as compared to that with cisplatin. Differences in cell morphology are suggested to be applied as quantitative markers of cell viability and cytostatic agent efficacy. The conclusions made are supported by a comparison with the results of recent experiments based on survival rates of laboratory animals treated with these agents.

show abstract

“…In order to examine the adequacy of the developed mathematical model we used fresh and frozen cranberries and cowberries and syrups with sucrose content of 30, 40 and 50%. Analysis of sucrose content in berries and syrups was carried out by refractometry technique, which is widely used in many research areas [14][15][16].…”

Section: Methodsmentioning

confidence: 99%

Modeling of Kinetics of Mass-Exchange Processes for Fruit and Berry Production Preservation

Bazarnova¹,

Кузнецова²,

Аронова³

2018

International Scientific and Practical Conference "AgroSMART - Smart Solutions for Agriculture" (AgroSMART 2018)

View full text Add to dashboard Cite

At preservation of fruits and berries mass-exchange processes take place in liquid mediums, which define quality of the canned production. The composition of covering syrup should guarantee preservation of turgor, taste and color of fruits and berries and prevent fermentation processes. We carried out construction and adequacy examination of kinetic model for moisture osmotic transfer for fruit-berry material to covering syrups at thermal and refrigerating preservation. We obtained empirical coefficients. It was established that moisture migration rate depends on berry type and syrup concentration. Moisture loss for more than 30% results in berries turgor loss, which makes its further storage impractical. Using the obtained equation and empirical constants, we have established that the duration of the refrigeration storage of cowberry canned in 30% sugar syrups for the stated conditions was about 130 days.

show abstract

Refractive index distributions in dehydrated cells of human oral cavity epithelium

Cited by 22 publications

References 35 publications

Super-resolution microscopy for biological specimens: lensless phase retrieval in noisy conditions

Super-resolution microscopy for biological specimens: lensless phase retrieval in noisy conditions

Morphological changes in the ovarian carcinoma cells of Wistar rats induced by chemotherapy with cisplatin and dioxadet

Modeling of Kinetics of Mass-Exchange Processes for Fruit and Berry Production Preservation

Contact Info

Product

Resources

About