This paper presents a methodology allowing for determination of strain soliton velocity in opaque solid materials. The methodology is based on the analysis of soliton evolution in a layer of a transparent material adhesively bonded to the layer of a material under study. It is shown that the resulting soliton velocity in the complex waveguide equals to the arithmetic mean of soliton velocities in the two component materials. The suggested methodology is best suited for analysis of materials with relatively close elastic parameters and can be applied in research of nonlinear wave processes in opaque composites on the basis of transparent matrices.
Changes in morphological characteristics of cells from two cultured cancer cell lines, HeLa and A549, induced by photodynamic treatment with Radachlorin photosensitizer have been monitored using digital holographic microscopy. The observed dose-dependent post-treatment dynamics of phase shift variations demonstrated several scenarios of cell death. In particular the phase shift increase at low doses can be associated with apoptosis while its decrease at high doses can be associated with necrosis. Two cell types were shown to be differently responsive to treatment at the same doses. Although the sequence of death scenarios with increasing irradiation dose was demonstrated to be the same, each specific scenario was realized at substantially different doses. Results obtained by holographic microscopy were confirmed by confocal fluorescence microscopy with the commonly used test assay.
Recent works demonstrated that digital time-resolved holography is the prospective approach to study nonlinear light-matter interaction processes. In this Letter, we present a straightforward inline holographic approach for studying degenerate phase modulation induced by an inclined collimated pump beam in the isotropic sample. The method is based on a minimization of the difference between experimentally acquired data and simulated inline holograms obtained from a numerical model of pump-probe interaction in optical nonlinear media. A sophisticated experimental data processing algorithm is implemented to provide high sensitivity and a signal-to-noise ratio eligible for soft interaction with a collimated pump beam. The integral phase shift determined by our method can be used to estimate the nonlinear refractive index and the relaxation time for material with a low damage threshold. We validated our approach for the case of soda-lime and BK7 glasses.
A methodology providing noninvasive monitoring and evaluation of the effect of photodynamic treatment on live cells in vitro is presented. Variations in morphological characteristics of cells in the course and after treatment are recorded by means of digital holographic microscopy. High-precision measurements of phase shift gained by probe radiation in HeLa and human endometrial mesenchymal stem cell cultures demonstrate for the first time changes of their volume occurred in response to treatment.
Digital holographic microscopy supplemented with the developed cell segmentation and machine learning and classification algorithms is implemented for quantitative description of the dynamics of cellular necrosis induced by photodynamic treatment in vitro. It is demonstrated that the developed algorithms operating with a set of optical, morphological, and physiological parameters of cells, obtained from their phase images, can be used for automatic distinction between live and necrotic cells. The developed classifier provides high accuracy of about 95.5% and allows for calculation of survival rates in the course of cell death.
In this paper, we present a novel numerical approach for increasing the resolution of retrieved images of objects after their diffraction patterns are recorded via terahertz pulse time-domain holography (THz PTDH). THz PTDH allows for spectrally resolved imaging with high spatial resolution and does not require the fine alignment of complex optics in the THz path. The proposed data post-processing method opens up the possibility to reconstruct holograms recorded with spatially restricted THz detectors, and overcome the diffraction limit even for the lower-frequency spectral components. The method involves an iterative procedure of backward-forward wavefront propagation to simulate the field distribution beyond the initially recorded hologram area. We show significant improvement in both the object reconstruction and contrast across the whole spectrum, with qualitative resolution enhancement at lower frequency spectral components.
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