Digital holographic microscopy in label-free analysis of cultured cells’ response to photodynamic treatment

Abstract

A methodology providing noninvasive monitoring and evaluation of the effect of photodynamic treatment on live cells in vitro is presented. Variations in morphological characteristics of cells in the course and after treatment are recorded by means of digital holographic microscopy. High-precision measurements of phase shift gained by probe radiation in HeLa and human endometrial mesenchymal stem cell cultures demonstrate for the first time changes of their volume occurred in response to treatment.

Quantitative assessment of changes in cellular morphology at photodynamic treatment in vitro by means of digital holographic microscopy

“…Cells were incubated in this solution for 4 hours, then the medium was replaced by the one without PS. As shown in [18,19] Radachlorin penetrates through the cellular membrane and accumulates mainly in mitochondria, lysosomes and endoplasmic reticulum. Basic photophysical properties of Radachlorin in aqueous solution were studied in our recent works [20,21], where the efficient generation of singlet oxygen was demonstrated.…”

“…[13,14,15,16,17]). In [18] we presented our first results on determination of cellular morphology at PD treatment by means of digital holographic microscopy. The necrotic pathway of cell death was investigated in two types of cell cultures: cancer and stem.…”

Исследование Показателя Преломления Обезвоженных Клеток С Помощью Цифровой Голографической Микроскопии

“…В последние годы цифровая голографическая микроскопия находит широкое применение в биологических исследованиях [1,2], а также в работах, связанных с медицинскими приложениями, в частности при диагностике целого ряда заболеваний [3,4]. В отличие от оптичес-кой микроскопии голографическая микроскопия позволяет получать количественные данные о фазовом запаздывании волнового фронта, прошедшего через клеточные структуры, что широко используется при наблюдении различных биологических процессов [5,6]. Измеряемой физической величиной в голографических методах является показатель преломления, определение пространственных распределений которого дает возможность получить важную дополнительную информацию по сравнению с другими оптическими методами.…”

Research summer camp in photonics

“…Liu Weidi participated in a project dedicated to improving the Terahertz Pulse Time-Domain Holography (THz PTDH) method that was proved perspective for phase imaging in the terahertz frequency range. 3 The work done by Weidi will allow increasing measurement speed by several times. Xia Zhenhi chose wavefront adaptive optimization through scattering media as her topic.…”