Reduction of oxidative stress by bioaugmented strain Pseudomonas sp. HF-1 and selection of potential biomarkers in sequencing batch reactor treating tobacco wastewater

Abstract

Oxidative stress induced by toxic pollutants is generally responsible for the poor performance of many sequencing batch reactors (SBRs) treating organic wastewater. In this study, the oxidative stress in two SBR systems that dealt with tobacco wastewater was monitored by measuring four popular biomarkers (superoxide dismutase, SOD; catalase, CAT; glutathione, GSH; and malondialdehyde, MDA). In the non-BA (non-bio-augmented) system, more intense oxidative stress was induced by a higher concentration of nicotine in tobacco wastewater, and excessive oxidative stress was induced by 250 mg/l of nicotine at the final stage. However, when a nicotine-degrading bacterial strain Pseudomonas sp. HF-1 was added to the BA (bio-augmented) system, the oxidative stress was significantly reduced compared to the non-BA system (p < 0.01).These results suggested that the oxidative stress was mainly induced by nicotine in the SBR treatment of tobacco wastewater, and that bioaugmentation with strain HF-1 would be a potential strategy to reduce the oxidative stress and thereby improve the performance in SBRs. Additionally, the positive correlation between the nicotine content and CAT, GSH and MDA activity in both systems implied that these parameters can be used as biomarkers for reflecting the performance of SBR treatment of tobacco wastewater, and in monitoring nicotine environmental pollution.

Unraveling the concentration-dependent metabolic response of Pseudomonas sp. HF-1 to nicotine stress by 1H NMR-based metabolomics

“…Nicotine is an important oxidative stressor to organisms in our current environment (Shao et al 2010). It is the principal tobacco alkaloid, accounting for 95 % of the total alkaloid content and 1.5 % of the total weight of cigarette tobacco (Benowitz et al 1983).…”

Differential Responses of the Antioxidant System of Ametryn and Clomazone Tolerant Bacteria

“…Lipid peroxidation is one of the best predictors of ROS level inducted under stress conditions [60], and can be used as a marker of oxidative stress [61]. An increased content of MDA was detected when strain CC07 was exposed to clomazone alone or in mixture with ametryn (Fig.…”

Simultaneous efficient removal of high‐strength ammonia nitrogen and chemical oxygen demand from landfill leachate by using an extremely high ammonia nitrogen–resistant strain

“…The successful implementation of bioaugmentation depends on the efficiency of the added culture to degrade the pollutant under natural conditions and to adapt to the indigenous strains . Genetically modified strains usually have the advantage to enhance the transformation or removal of specific pollutants , but the competition between exogenous and indigenous microbiota may be responsible for unsuccessful bioaugmentation processes . Thus, isolating, culturing, and inoculating the dominant microorganisms may enhance their survival chance relative to other microbes, and consequently could have a desirable effect on removal efficiency.…”