Simultaneous efficient removal of high‐strength ammonia nitrogen and chemical oxygen demand from landfill leachate by using an extremely high ammonia nitrogen–resistant strain

Abstract

Bioaugmentation is a promising technology for pollutant elimination from stressed environments, and it would provide an efficient way to solve challenges in traditional biotreatment of wastewater with high strength of ammonia nitrogen (NH4(+)-N). A high NH4(+)-N-resistant bacteria strain, identified as Bacillus cereus (Jlu BC), was domesticated and isolated from the bacteria consortium in landfill leachate. Jlu BC could survive in 100 g/L NH4(+)-N environment, which indicated its extremely high NH4(+)-N tolerance than the stains found before. Jlu BC was employed in the bioaugmented system to remove high strength of NH4(+)-N from landfill leachate, and to increase the removal efficiency, response surface methodology (RSM) was used for optimizing bioaugmentation degradation conditions. At the optimum condition (initial pH 7.33, 4.14 days, initial chemical oxygen demand [COD] concentration [18,000 mg/L], 3.5 mL inoculated domesticated bacteria strain, 0.3 mg/mL phosphorus supplement, 30 °C, and 170 rpm), 94.74 ± 3.8% removal rate of NH4(+)-N was obtained, and the experiment data corresponded well with the predicted removal rate of the RSM models (95.50%). Furthermore, COD removal rate of 81.94 ± 1.4% was obtained simultaneously. The results presented are promising, and the screened strain would be of great practical importance in mature landfill leachate and other NH4(+)-N enrichment wastewater pollution control.

Optimal reduction of chemical oxygen demand and NH3–N from landfill leachate using a strongly resistant novel Bacillus salmalaya strain

“…Removal efficiency of NH 3 –N remarkably increased after the third day (47%) and improved until the last day of study. In a similar study, [ 6 ] reported that under optimum conditions (30 °C, pH 7.33, 4.14 days, and 170 rpm), 94.7% of NH 4 + –N was removed from landfill leachates that were treated with 3.5 mL of inoculated domesticated bacteria. The same trend was obtained for the sterilized samples (Fig.…”

“…A serious issue of landfill leachate is its high content of ammonia nitrogen (NH 3 –N), which prevents the activity and growth of microorganisms due to accelerate eutrophication and an increased dissolved oxygen reduction as well as toxic effects on aquatic organisms. Conventional treatment methods are challenging, especially for mature or old landfill leachate with NH 3 –N levels [ 6 – 8 ]. Organic matter in leachate is quantified by biochemical oxygen demand (BOD) and chemical oxygen demand (COD).…”

Modeling a Three-Stage Biological Trickling Filter Based on the A2O Process for Sewage Treatment

“…In the BTF process, several factors such as temperature, dissolved oxygen (DO), and reflux ratio (RR) can have impacts on treatment performance [23][24][25]. Therefore, the reliability of the system can be increased by a multistage BTF based on the A2O process (A2O-BTF).…”