Reduction of mucin-1 gene expression associated with increased Escherichia coli adherence in the canine uterus in the early stage of dioestrus

Ishiguro, Kayoko; Baba, Eiichiroh; Torii, Ryuzo; Tamada, Hiromichi; Kawate, Noritoshi; Hatoya, Shingo; Wijewardana, Viskam; Kumagai, D.; Sugiura, Kikuya; Sawada, Tsutomu; Inaba, Toshio

doi:10.1016/j.tvjl.2005.11.009

Cited by 29 publications

(19 citation statements)

References 21 publications

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“…The claim that some breeds are more prone to pyometra than others (Niskanen and Thrusfield, 1998) was not observed in our study due to an insufficient number of patients. The period between the last proestrus bleeding and the onset of pyometra signs was similar to some previous reports (Tsumagari et al, 2005;Ishiguro et al, 2007). However, this time period was markedly longer in 11 bitches in our study than some researchers' reports (Tsumagari et al, 2005;Ishiguro et al, 2007).…”

Section: Clinical Signs and Bacteriologysupporting

confidence: 90%

The comparative evaluation of serum biochemical, haematological, bacteriological and clinical findings of dead and recovered bitches with pyometra in the postoperative process

Küplülü¹,

Vural²,

Demırel³

et al. 2009

Acta vet. (Beogr.)

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The aim of this study was to investigate the prognosis, the risk status and the death reasons in dead and recovered bitches with pyometra after the operative approach by comparing serum biochemical, and haematological parameters, as well as bacteriological and clinical findings. Bitches with pyometra (n=30) were ovariohysterectomized. Eight bitches that died after the ovariohysterectomy formed Group 1 and the rest, which recovered, was Group 2. In addition, 10 healthy bitches were used as the reference for haematological and biochemical parameters. It was found that the level of band neutrophils in Group 1 was higher than 10% and there was lymphopenia along with a marked monocytosis resulting in a highly negative correlation (r=-0.74). There were significant differences (p<0.01) between Group 1 and 2 for BUN, creatinine and BUN/creatinine ratio. Also, the increased serum BUN and creatinine levels were significantly correlated (rs = 0.605 and rs = 0.514, respectively). A significant tendency was apparent for increasing mortality in bitches with pyometra whose BUN levels were over 30 mg/dL (odds ratio=23.80) and creatinine levels above 1.5 mg/dL (odds ratio=7.518). Clinical signs and bacteriological findings did not lead to marked differences between the groups. In pyometra cases, the elderly azotemic dogs having higher BUN and creatinine concentration, would more likely succumb within 3 days after surgery. It may be concluded that the increased BUN concentrations may serve as a reliable prognostic predictor

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Section: Clinical Signs and Bacteriologysupporting

confidence: 90%

The comparative evaluation of serum biochemical, haematological, bacteriological and clinical findings of dead and recovered bitches with pyometra in the postoperative process

Küplülü¹,

Vural²,

Demırel³

et al. 2009

Acta vet. (Beogr.)

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“…could also been detected [11,22,23] . It has been established that if E. coli migrates to the uterus, it binds with higher affinity to the endometrium and myometrium layers and constitutes a resistance to the uterus' defense mechanisms such as lactoferrin and mucin-1 [24][25][26][27] . As reported in previous studies [4,11,23,28] , gram negative bacteria (75%) such as E. coli, Klebsiella spp.…”

Section: Discussionmentioning

confidence: 99%

Köpeklerde Endotoksik Pyometranın Klinik, Bakteriyolojik ve Histopatolojik Özellikleri

Demırel¹,

Vural²,

Vural³

et al. 2018

Kafkas Univ Vet Fak Derg

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The object of this study was to compare the histopathologic change in uterus as well as the findings of clinical and bacteriological of twentyfour bitches with pyometra. Uterine lesions were grouped as acute endometritis, subacute endometritis, acute metritis, subacute metritis. Complete blood-count, blood biochemical parameters and toxin levels were determined. Only alkaline phosphatase levels were significantly different between all groups (P<0.01). Blood endotoxin level was different between the acute and subacute endometritis groups (P<0.01), and subacute endometritis and acute metritis groups (P<0.01). All bitches in the subacute endometritis group recovered. Three bitches in the acute endometritis group, two bitches in the acute metritis group and one bitch in the subacute metritis group died. The blood endotoxin level was determined to be quite high in the dead bitches. There was a difference in the levels of blood urea nitrogen (P<0.05), creatinine (P<0.01), and toxin (P<0.0001) between the dead and surviving bitches. In conclusion, the prognosis of pyometra may be recovered in bitches diagnosed with subacute endometritis associated with low levels of circulating endotoxin. The endotoxemic bitches that have blood endotoxin level above 0.96 EU/mL along with significantly increased alkaline phosphatase, blood urea nitrogen, and creatinine concentration would likely end up with a mortality outcome for acute endometritis, acute metritis, or subacute metritis. These parameters may serve as good prognostic markers in bitches with pyometra. Keywords: Bacteriology, Bitch, Histopathology, Prognosis, Pyometra Köpeklerde Endotoksik Pyometranın Klinik, Bakteriyolojik ve Histopatolojik Özellikleri ÖzBu çalışmanın amacı, pyometralı yirmi dört köpeğin uteruslarındaki histopatolojik değişikliklerin karşılaştırılmasıdır. Uterus lezyonları akut endometritis, subakut endometritis, akut metritis, subakut metritis olarak gruplandırıldı. Tam kan sayımı, kan biyokimyasal parametreler ve toksin seviyeleri belirlendi. Tüm gruplar arasındaki farklılık sadece alkalen fosfotaz seviyesinde önemliydi (P<0.01). Kan endotoksin seviyesi, akut ve subakut endometritis grupları (P<0.01) ile subakut endometritis ve akut metritis grupları (P<0.01) arasında farklıydı. Subakut endometritis grubundaki tüm köpekler iyileşti. Akut endometritis grubunda üç köpek, akut metritis grubunda iki köpek ve subakut metritis grubunda ise bir köpek öldü. Kan endotoksin seviyesinin ölen köpeklerde oldukça yüksek seviyede olduğu belirlendi. Ölen ve yaşayan köpekler arasında kan üre nitrojen (P<0.05), kreatinin (P<0.01) ve toksin (P<0.0001) seviyelerinin farklı olduğu görüldü. Sonuç olarak, dolaşımdaki endotoksinin düşük seviyesi ile ilişkili olarak subakut endometritis tanısı konulan köpeklerde pyometranın prognozunun iyileştirilebileceği düşünüldü. Yüksek seviyede alkalen fosfotaz, kan üre azotu ve kreatinin konsantrasyonu ile birlikte 0.96 EU/mL'nin üstündeki kan endotoksin düzeyine sahip endotoksemik köpeklerde akut endometritis, akut metritis veya ...

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“…The relationship between adherence of Escherichia coli and expression of MUC1 in the endometrium was studied in bitches at different stages of the estrus cycle: MUC1 gene expression markedly decreased in the canine endometrium at the early stage of diestrus, resulting in increased E. coli adherence. This could be one of the factors inducing pyometra (Ishiguro et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Sheep MUC1 Gene: Structure of the Repetitive Region and Polymorphism

Rasero

Bianchi

Cauvin

et al. 2007

Journal of Dairy Science

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An investigation was undertaken with the aim of studying the repetitive region of the MUC1 gene and analyzing its polymorphisms in some Italian sheep breeds. Two primers previously used for the goat MUC1 gene analyses allowed for the amplification of 4 different alleles. The sequence analysis showed that the repetitive region of the sheep MUC1 gene is an array of 60-bp repeats, in accordance with the information reported in humans, cattle, and goats. The polypeptide sequence encoded by the consensus repeat was very similar to the corresponding sequences of goats and cattle. The average homology of all repeated units was 82%; when the repeats were compared with the derived consensus repeat, homology dropped to 78%. The repeats were not all perfectly conserved, but the sequence homology was nevertheless clearly sufficient to preserve the mechanism giving rise to the variable-number tandem-repeat polymorphism. In spite of their reduced sequence homology, the sheep repeats shared a high number of potential glycosylation sites. The conservation of the exact number and position of glycosylation sites did not seem to be very important for the purpose of functional integrity, but glycosylation appeared to be conserved as a bulk property. Analysis of the polymorphism in 6 Italian breeds showed that the sheep repetitive region seemed to be less variable and smaller in size than the repetitive region of the goat. The findings of this study suggest that ruminants can be a useful model to study the mechanisms by which the variation in the repeat number and the extracellular domain size can modulate the effectiveness of MUC1 as a cell-surface shield.

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Reduction of mucin-1 gene expression associated with increased Escherichia coli adherence in the canine uterus in the early stage of dioestrus

Cited by 29 publications

References 21 publications

The comparative evaluation of serum biochemical, haematological, bacteriological and clinical findings of dead and recovered bitches with pyometra in the postoperative process

The comparative evaluation of serum biochemical, haematological, bacteriological and clinical findings of dead and recovered bitches with pyometra in the postoperative process

Köpeklerde Endotoksik Pyometranın Klinik, Bakteriyolojik ve Histopatolojik Özellikleri

Analysis of the Sheep MUC1 Gene: Structure of the Repetitive Region and Polymorphism

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