Reduction of bacteria and human immunodeficiency virus Type 1 infectivity of platelet suspension in plasma using xenon flash‐pulse light in a bench‐scale trial

Abstract

Xe flash phototreatment could prevent bacterial proliferation and reduce HIV-1 infectivity in 100% plasma PCs without any additional compounds, but enhanced PLT storage lesions. Further improvement is required to increase the potency of pathogen inactivation with reducing PLT damage.

Pulsed xenon flash treatment inactivates bacteria in apheresis platelet concentrates while preserving in vitro quality and functionality

“…Two bacteria Staphylococcus aureus NBRC 13276 and Streptococcus dysgalactiae subsp. equisimilis ATCC 12394 were used because they were relatively resistant in the previous inactivation study . PCs within 4 days after collection were spiked with bacteria at an initial titer ranging from 8 to 108 colony‐forming units (CFUs) per bag.…”

“…PCs within 4 days after collection were spiked with bacteria at an initial titer ranging from 8 to 108 colony‐forming units (CFUs) per bag. Bacterial titer or growth was measured by the pour plate culture method as previously described …”

“…PC variables were measured according to the previous study . Briefly, PLT concentration and mean PLT volume (MPV) were measured using an automated hematology analyzer (XS‐800i, Sysmex), glucose concentration and pH at 22°C by an automated gas analyzer (RapidPoint 405; Siemens Healthcare Diagnostics), and HSR according to the method described by Holme and colleagues …”

“…Particle size distribution was analyzed by an injection flow particle image analyzer (IF‐200nano; Jasco) in triplicate measurement as previously described . Max distance, one of the parameters of the analyzer which represented the longest diameter of elliptic particles, was defined as the particle size.…”

“…These methods require the addition of chemical compounds (INTERCEPT and Mirasol) or replacement of plasma with PLT additive solution (AS; THERAFLEX UV‐Platelets). Therefore, we have previously investigated a novel method using pulsed xenon (Xe) flash, which requires neither the addition of compounds nor the dilution of plasma, and revealed that some bacterial strains spiked into PLT suspension in 100% plasma could be inactivated to sterility over 6 storage days . However, the method had limitations in bactericidal ability depending on the initial titer of contamination and bacterial strain or species.…”