Altered activation of integrin ɑIIbβ3 on platelets irradiated with ultraviolet C from pathogen-reducing xenon flash

Abe, Hideki; Shiba, Masayuki; Satake, Masahiro

doi:10.1016/j.transci.2019.05.001

Cited by 2 publications

(2 citation statements)

References 16 publications

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“…Swirling was maintained in both the XeF‐FP–treated and nontreated PCs until the end of the storage, suggesting that a discoid shape was maintained in both products . Furthermore, we previously showed that PAC‐1–positive PLTs induced by the UVC irradiation from Xe flash did not bind to fibrinogen under resting condition in suspension . The changes in the above parameters in XeF‐FP–treated PCs were statistically significant as compared with those in the nontreated ones, but the clinical significance of these changes is unclear at present.…”

Section: Discussionmentioning

confidence: 89%

Flow path system of ultraviolet C irradiation from xenon flash to reduce bacteria survival in platelet products containing a platelet additive solution

et al. 2020

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BACKGROUND: Our previous study showed that ultraviolet C (UVC) from xenon (Xe) flash without any photoreactive compounds inactivated bacteria in platelet concentrates (PCs) with less damage to platelets (PLTs) as compared with Xe flash containing ultraviolet A, ultraviolet B, and visible light. Here, we report a UVC irradiation system for PCs under flow conditions consisting of a flow path-irradiation sheet, a peristaltic pump, and a collection bag. STUDY DESIGN AND METHODS: Plateletconcentrates containing Ringerʼs solution (R-PCs) inoculated with bacteria were injected into a flow path sheet using a peristaltic pump, being irradiated with UVC from Xe flash. The quality of the irradiated PCs containing platelet additive solution (PAS-PCs) was assessed based on PC variables, PLT surface markers, and aggregation ability. RESULTS: Streptococcus dysgalactiae (12 tests) andEscherichia coli (11) were all negative on bacterial culture, while Staphylococcus aureus (12) and Klebsiella pneumoniae (14) grew in one and two R-PCs, respectively. Bacillus cereus spores were inactivated in 7 of 12 R-PCs. PC variables became significantly different between irradiated and nonirradiated PAS-PCs. P-selectin, first procaspase-activating compound (PAC-1) binding, and phosphatidylserine increased by irradiation. Aggregability stimulated by adenosine diphosphate, collagen, or thromboxane A 2 increased in the irradiated PAS-PCs, while that by thrombin became smaller compared with nonirradiated controls.CONCLUSION: This newly developed system inactivated bacteria including spores in R-PCs. PAS-PCs irradiated by this system retained acceptable in vitro quality and aggregability. Usage of a peristaltic pump instead of agitator during irradiation may enable this system to be directly combined with an apheresis blood cell separator. E ven in the developed countries, transfusiontransmitted bacterial infection remains a serious lifethreatening adverse event following the transfusion of platelet concentrates (PCs). PC stored at a temperature of 20 to 24°C is susceptible to bacterial growth, although its incidence is quite low. In 2017, a fatal sepsis case caused by the transfusion of a PC contaminated with Escherichia coli was reported to the Japanese Red Cross Blood Service Headquarters, 1 although efforts to eliminate bacterial contamination have been promoted by a combination of improved skin disinfection and the diversion of initial blood flow at blood donation, 2 in addition to a short shelf life of PCs (3 days) in Japan.A longer shelf life of PCs (≥5 days) in many countries makes inventory management easier. Automated culture systems, such as BacT/ALERT 3D (BioMérieux), have been widely implemented in those countries for the detection of bacterial contamination in PCs by sampling an aliquot of PCs within ABBREVIATIONS: ADP = adenosine diphosphate; ANOVA = analysis of variance; FDA = US Food and Drug Administration; FITC = fluorescein isothiocyanate; HSR = hypotonic shock response; ihPAS = in-house platelet additive solution; MPV = mean pla...

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Section: Discussionmentioning

confidence: 89%

Flow path system of ultraviolet C irradiation from xenon flash to reduce bacteria survival in platelet products containing a platelet additive solution

et al. 2020

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“…Our study showed no difference in the adhesion of PLTs to fibrinogen between flash UVC‐irradiated and nonirradiated groups. We previously reported that fibrinogen in soluble form bound to flash UVC‐irradiated PLTs upon ADP stimulation, although it did not bind to irradiated PLTs without ADP stimulation under liquid‐phase conditions 20 . These results suggest that flash UVC irradiation did not affect αIIbβ3‐fibrinogen interaction and subsequent signals in our limited observations.…”

Section: Discussionmentioning

confidence: 40%

In vitro thrombus formation and in vivo hemostasis mediated by platelets irradiated with bactericidal ultraviolet C from xenon flash under flow conditions

et al. 2020

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Background We previously reported a flow path–ultraviolet C (UVC) irradiation system for platelet concentrates (PCs) with platelet additive solution (PAS) to minimize contamination by bacteria. Here, we investigated functionalities of irradiated platelets (PLTs) in in vitro thrombus formation and in vivo hemostasis. Study Design and Methods PAS‐PCs were irradiated with flash UVC using the flow path system. Their variables (PLT count, mean platelet volume, pH, glucose, lactate, glycoprotein [GP] Ib, and activated integrin αIIbβ3) were evaluated. Static adhesion to collagen or fibrinogen was analyzed using fluorescent microscopy. Thrombus formation under flow conditions was assessed using a collagen‐coated bead column. Adenosine diphosphate (ADP)‐induced Akt phosphorylation was determined by western blot. In vivo hemostasis and circulatory survival of PLTs were assessed with a rabbit bleeding model. Results All variables, except for GPIb expression, were slightly, but significantly, impaired after flash UVC irradiation throughout the 6‐day storage period. No difference was observed in static adhesion to either collagen or fibrinogen between irradiated and nonirradiated PAS‐PCs. In vitro thrombus formation of flash UVC‐irradiated PAS‐PCs was significantly greater than that of nonirradiated PAS‐PCs. ADP‐induced Akt phosphorylation was enhanced in irradiated PAS‐PCs. In vivo hemostatic efficacy was comparable between the groups on Day 1. The efficacy declined in nonirradiated PAS‐PCs on Day 5, while it was retained in flash UVC‐irradiated PAS‐PCs. Circulatory survival of PLTs was lower in irradiated PAS‐PCs. Conclusions PAS‐PCs irradiated with UVC from xenon flash have favorable properties to achieve hemostasis compared with nonirradiated PAS‐PCs.

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Altered activation of integrin ɑIIbβ3 on platelets irradiated with ultraviolet C from pathogen-reducing xenon flash

Cited by 2 publications

References 16 publications

Flow path system of ultraviolet C irradiation from xenon flash to reduce bacteria survival in platelet products containing a platelet additive solution

Flow path system of ultraviolet C irradiation from xenon flash to reduce bacteria survival in platelet products containing a platelet additive solution

In vitro thrombus formation and in vivo hemostasis mediated by platelets irradiated with bactericidal ultraviolet C from xenon flash under flow conditions

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