Pulsed xenon flash treatment inactivates bacteria in apheresis platelet concentrates while preserving in vitro quality and functionality

Abstract

The fXe treatment inactivated bacteria in apheresis PCs in plasma without additional chemical compounds. The fXe-treated PCs retained acceptable in vitro properties of PC quality and PLT functionality.

Restored response to ADP downstream of purinergic P2Y12 receptor in apheresis platelets after pathogen‐reducing xenon flash treatment

“…In our previous study, we found that fXe-treated PLTs showed sustained aggregation in contrast to untreated blood products PLTs collected by apheresis, which showed reversible aggregation in response to ADP (10 mM), and the sustained aggregability of fXe-PLTs was maintained during 6-day storage. 10 We initially found that the change of the aggregation pattern in Aph-PLTs from reversible to sustained depended on the fXe flash dose (Fig. 1).…”

“…[7][8][9] Recently, we newly developed a method to prevent bacterial growth experimentally spiked into apheresis PCs using filtered-xenon (Xe) flash irradiation, named fXe treatment. 10 PCs in a UV-permeable bag were irradiated with UVC (wavelength of 200-290 nm) of Xe flash, filtered to exclude UVA, UVB, and visible light. Unlike INTERCEPT and Mirasol, fXe treatment requires no additional chemical compounds, but it is still in the experimental stage.…”

“…Aph‐PLTs was used within 15 to 21 hours after donation. To prepare filtered Xe flash‐treated PLTs (fXe‐PLTs), a 1‐mL aliquot of paired apheresis PCs was dispensed into a 35‐mm culture dish and irradiated with filtered Xe flash (UVC at a wavelength of 200‐290 nm) through the band‐stop filter U250/330 in order to exclude UVA, UVB, and visible light, as previously described . The irradiation equipment used in this study yielded 0.073 mJ/cm 2 /shot.…”

“…To prepare filtered Xe flash-treated PLTs (fXe-PLTs), a 1-mL aliquot of paired apheresis PCs was dispensed into a 35-mm culture dish and irradiated with filtered Xe flash (UVC at a wavelength of 200-290 nm) through the band-stop filter U250/330 in order to exclude UVA, UVB, and visible light, as previously described. 10 The irradiation equipment used in this study yielded 0.073 mJ/cm 2 /shot. Platelet-rich plasma (PRP) as freshly prepared PLTs (PRP-PLTs) was fractionated from ACD-collected whole blood by centrifugation at 200 3 g for 15 minutes followed by 100 3 g for 10 minutes, and was used within 2 to 7 hours after collection.…”

“…Recently, we newly developed a method to prevent bacterial growth experimentally spiked into apheresis PCs using filtered‐xenon (Xe) flash irradiation, named fXe treatment . PCs in a UV‐permeable bag were irradiated with UVC (wavelength of 200‐290 nm) of Xe flash, filtered to exclude UVA, UVB, and visible light.…”