Reduction of 3T3 fibroblast adhesion on SS316L by methyl-terminated SAMs

Abstract

Inhibiting the non-specific adhesion of cells and proteins to biomaterials such as stents, catheters and guide wires is an important interfacial issue that needs to be addressed in order to reduce surface-related implant complications. Medical grade stainless steel 316L was used as a model system to address this issue. To alter the interfacial property of the implant, self assembled monolayers of long chain phosphonic acids with −CH3, −COOH, −OH tail groups were formed on the native oxide surface of medical grade stainless steel 316L. The effect of varying the tail groups on 3T3 fibroblast adhesion was investigated. The methyl terminated phosphonic acid significantly prevented cell adhesion however presentation of hydrophilic tail groups at the interface did not significantly reduce cell adhesion when compared to the control stainless steel 316L.

Phosphonate coupling molecules for the control of surface/interface properties and the synthesis of nanomaterials

“…Surface modification by phosphonate monolayers has been applied to a large variety of oxide substrates, notably titania, 8,9,12,50,51 alumina, 50,52,53 iron oxides, 10,[54][55][56] indium-tin oxide (ITO), 11,27,57 and silica. 18,58,59 PAs also react with the (native) oxide layer at the surface of metals and alloys 15 such as titanium 60,61 and titanium alloys (Ti-6Al-4V, Ti-45Nb, nitinol), [62][63][64][65] aluminum, 66,67 copper, 68,69 silver, 70 steel, [71][72][73] magnesium alloys [74][75][76] and cobalt-chromium alloys. 77,78 PAs have also been used for grafting the interlayer surface of lamellar materials such as layered double hydroxides or perovskites.…”

“…Inhibiting non-specific adhesion of cells and proteins to biomaterials is an important issue. Raman et al 71 have shown that phosphonate monolayers could be used to control the non-specific adhesion of cells and proteins onto stainless steel (SS316L). Hydrophobic octadecylphosphonic SAMs reduced the non-specific fibroblast cell adhesion by 77% whereas hydrophilic PA SAMs terminated by -OH and -COOH groups were not significantly different from a SS316L control.…”

Antimicrobial Activity of Nitric Oxide-Releasing Ti-6Al-4V Metal Oxide

“…Live and dead cells were counted on each microscope image and the average percent viability was calculated [41]. The resulting percent viabilities were normalized to the fibroblasts grown on unmodified Ti-6Al-4V.…”

“…The cell viability of NIH3T3 fibroblasts grown on S -nitroso-penicillamine-modified Ti-6Al-4V was determined using a Live/Dead ® Viability/Cytotoxicity assay. Live and dead cells were counted on each microscope image and the average percent viability was calculated [ 41 ]. The resulting percent viabilities were normalized to the fibroblasts grown on unmodified Ti-6Al-4V.…”

“…The number of live and dead cells was counted on 45 images for each sample and control ( n = 45). The percent viability for each Ti-6Al-4V sample was calculated by dividing the number of live cells by the total number of cells (live and dead) and was normalized to the fibroblast control [ 41 ].…”