Reduction in levels of deoxynivalenol in contaminated wheat by chemical and physical treatment

Young, J. Christopher; Subryan, L. M.; Potts, David M.; McLaren, M.; Gobran, F. H.

doi:10.1021/jf00069a021

Cited by 77 publications

(50 citation statements)

References 3 publications

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“…One possibility for the reduction of DON is the chemical treatment of cereals before feeding as already investigated in the 1980s. In a study by Young et al (1986), it was shown that the treatment of contaminated cereals with sodium bisulfite (SBS) is most effective in reducing the DON concentrations: less than 2% of the initial concentration of DON was detected after treatment of DON-contaminated wheat with SBS. During SBS-treatment, DON reacts with SBS in a 1,4-Michael addition yielding DON-sulfonate (DONS) (Fig.…”

Section: Introductionmentioning

confidence: 99%

Determination of deoxynivalenol-sulfonate (DONS) in cereals by hydrophilic interaction chromatography coupled to tandem mass spectrometry

et al. 2010

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Deoxynivalenol (DON) is one of most widespread mycotoxins in cereal commodities, and animal feed is prevalently contaminated at high concentrations. This poses a problem in animal nutrition as especially pigs are very sensitive to DON. An effective process for the reduction of the DON concentration is the treatment of contaminated feed with sodium bisulfite (SBS) whereby DON is transformed into DON-sulfonate (DONS). Although the success of this treatment has been confirmed in several feeding studies, it is unexplained if the decrease of DON is accompanied with a coincident increase of DONS. For this reason, we developed a method for the analysis of DONS using hydrophilic interaction chromatography coupled to tandem mass spectrometry. In order to investigate the correlation between DON and DONS concentrations during SBS-treatment, DON-contaminated wheat was treated with SBS and stored for up to 36 days. At defined timepoints of this treatment, samples were analyzed for DON and DONS using stable isotope labeled standards. The preparation, purification, and structure elucidation of DONS, and the HILIC-HPLC-MS/MS method for the analysis of DONS as well as the results of two storage experiments are presented in this paper.

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Section: Introductionmentioning

confidence: 99%

Determination of deoxynivalenol-sulfonate (DONS) in cereals by hydrophilic interaction chromatography coupled to tandem mass spectrometry

et al. 2010

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“…Therefore, it is suitable that detoxification methods are developed for the inactivation of toxins produced during the pre‐harvest stages of crop production. Although physical and chemical detoxification methods have been reported (Young et al. 1986; Trenholm et al.…”

Section: Introductionmentioning

confidence: 99%

Binding of Fusarium mycotoxins by fermentative bacteria in vitro

2006

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Aims: Fusarium toxins can occur in conserved forages impairing farm animal performances and health. On‐farm biological decontamination methods could be an alternative to traditional physico‐chemical methods. In this work, the ability to remove Fusarium toxins by fermentative bacteria was evaluated in vitro. Methods and Results: Twenty‐nine strains of lactic (LAB) and propionic acid bacteria (PAB) were tested for their ability to remove deoxynivalenol (DON) and fumonisins B1 and B2 (FB1, FB2) from an acid, pH 4, medium. Mycotoxin removal was widespread for LAB, but differences among strains were large. Removal was up to 55% for DON, 82% for FB1 and 100% for FB2. Selected strains were also capable of removing up to 88% zearalenone. The PAB strains were less efficient than the LAB. Binding, not biodegradation appeared to be the mode of action, as no toxin derivatives were observed and removal was not impaired in nonviable bacteria. Binding was not affected by pH, except for fumonisins that decreased to nearly 0% at neutral pH. Conclusions: Selected fermentative bacteria are able to bind main Fusarium mycotoxins. Significance and Impact of the Study: The binding ability of selected strains could be used to decrease the bioavailability of toxins in contaminated silages.

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“…Grain harvesting and post harvest strategies such as modifying combine harvester settings and the use of screening, cleaning, and aspiration techniques to removal shriveled, light-weight, and small-sized kernels have also been recommended as ways of further reducing losses due to FHB and DON (16,22,24,39,52). Grain harvesting and post harvest strategies such as modifying combine harvester settings and the use of screening, cleaning, and aspiration techniques to removal shriveled, light-weight, and small-sized kernels have also been recommended as ways of further reducing losses due to FHB and DON (16,22,24,39,52).…”

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confidence: 99%

Efficacy and Economics of Integrating In-Field and Harvesting Strategies to Manage Fusarium Head Blight of Wheat

2014

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A b s tra c t Salgado, J. D., Madden, L. V., and Paul, P. A. 2014. Efficacy and economics of integrating in-field and harvesting strategies to manage Fusarium head blight of wheat. Plant Dis. 98:1407-1421.Fusarium head blight (FHB), a fungal disease of wheat caused by Fusarium graminearum, and its associated toxins, particularly deoxynivalenol (DON), are best managed by integrating multiple strategies. Experiments were established in 2011 and 2013 to evaluate the effects of integrating cultivar resistance, fungicide application, and grain har vesting strategy on FHB index (IND; field severity), DON, grain yield (YLD), and grain test weight (TW; weight per unit volume). Plots of two moderately resistant and two susceptible cultivars were either treated with 19% tebuconazole + 19% prothioconazole or left un treated, and then inoculated with F. graminearum. IND was rated as the mean percentage of diseased spikelets per spike. Separate subsets of the plots of each cultivar-treatment combination were harvested with one of two combine harvester configuration: C 1 (the default, set at a fan speed of 1,375 rpm and a shutter opening of 70 mm) and C4 (modified, with the same fan speeds but a wider shutter opening of 90 mm). YLD and TW data were collected, and grain samples were rated for percent Fusarium-damaged kernels (FDK) and tested for DON. Results from linear mixed-model analyses showed that the cultivartreatment interaction was significant for all FHB-related responses, with the magnitude of the difference in mean arcsine-square-root-trans formed IND and FDK (arcIND and arcFDK) and log-transformed DON (logDON) between treated and untreated being higher for sus ceptible than moderately resistant cultivars. Plots harvested with the C4 combine configuration had significantly higher mean TW than those harvested with Cl. Treated plots had significantly higher YLD and TW than untreated plots, regardless of cultivar and configuration. Relative to the reference management program (untreated, susceptible cultivar, harvested with Cl), the greatest percent reduction in FDK and DON and increase in YLD was observed for programs that included moderate resistance and fungicide treatment. The greatest percent increase in TW relative to the reference was observed when C4 ad justed combine setting was integrated with resistance and fungicide. Overall, the most effective management programs all included fungi cide treatment, two included moderate resistance, and two included C4 combine setting. Relative to the reference management program, these programs resulted in 30 to 51% reduction in total estimated price dis count, $127 to 312 ha-1 increase in gross cash income, and economic benefit of $31 to 272 ha-1, depending on the level of FHB IND (5 to 15%), grain price ($118 to 276 metric ton-1), and fungicide application cost ($40 to 96 ha-1).

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Reduction in levels of deoxynivalenol in contaminated wheat by chemical and physical treatment

Cited by 77 publications

References 3 publications

Determination of deoxynivalenol-sulfonate (DONS) in cereals by hydrophilic interaction chromatography coupled to tandem mass spectrometry

Determination of deoxynivalenol-sulfonate (DONS) in cereals by hydrophilic interaction chromatography coupled to tandem mass spectrometry

Binding of Fusarium mycotoxins by fermentative bacteria in vitro

Efficacy and Economics of Integrating In-Field and Harvesting Strategies to Manage Fusarium Head Blight of Wheat

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