Binding of Fusarium mycotoxins by fermentative bacteria in vitro

Abstract

Aims: Fusarium toxins can occur in conserved forages impairing farm animal performances and health. On‐farm biological decontamination methods could be an alternative to traditional physico‐chemical methods. In this work, the ability to remove Fusarium toxins by fermentative bacteria was evaluated in vitro. Methods and Results: Twenty‐nine strains of lactic (LAB) and propionic acid bacteria (PAB) were tested for their ability to remove deoxynivalenol (DON) and fumonisins B1 and B2 (FB1, FB2) from an acid, pH 4, medium. Mycotoxin removal was widespread for LAB, but differences among strains were large. Removal was up to 55% for DON, 82% for FB1 and 100% for FB2. Selected strains were also capable of removing up to 88% zearalenone. The PAB strains were less efficient than the LAB. Binding, not biodegradation appeared to be the mode of action, as no toxin derivatives were observed and removal was not impaired in nonviable bacteria. Binding was not affected by pH, except for fumonisins that decreased to nearly 0% at neutral pH. Conclusions: Selected fermentative bacteria are able to bind main Fusarium mycotoxins. Significance and Impact of the Study: The binding ability of selected strains could be used to decrease the bioavailability of toxins in contaminated silages.

Ability ofLactobacillus rhamnosusGAF01 to remove AFM1in vitroand to counteract AFM1immunotoxicityin vivo

“…Results of several studies suggest that binding is the main mechanism of detoxification against aflatoxins (Niderkorn et al, 2006;Gratz, 2007). Strains L. rhamnosus GG and LC-705 seem to be the most effective in such detoxifications (Lahtinen et al, 2004).…”

Dairy Propionibacteria: Less Conventional Probiotics to Improve the Human and Animal Health

“…The capacity of Propionibacterium freudenreichii strain JS used alone and combined with lactobacilli (L. rhamnosus GG or LC705) to remove mycotoxins has been studied by in vitro [210][211][212], ex vivo [211] and in vivo assays [213][214]. It has been determined that both viable and heat-killed forms of propionibacteria are able to remove efficiently aflatoxin B1, fumonisins and trichotecenes from liquid media.…”

“…Mycotoxins are also forage contaminants, which impair animal performances and health. Several probiotic bacteria, commonly used in food products, have been shown to bind Aflatoxin B1 and the toxins produced by Fusarium sp such as zearalenone, fumonisins B1 and B2 and trichothecenes, like deoxynivalenol (DON), nivalenol (NIV) and T-2 toxin (T-2) preventing their absorption in the gastrointestinal tracts of animals and humans [210][211][212][213][214].…”

Biological Control of Molds and Mycotoxins in Foods

“…L. rhamnosus LC-705 removed only deoxynivalenol and diacetoxyscirpenol, with removal varying from 10 to 64%. Niderkorn et al (60) screened lactic and propionic acid bacteria for their ability to remove deoxynivalenol and fumonisin from solution and they found that it was strain specific, with propionic acid bacteria being less efficient than LAB. L. rhamnosus removed up to 55% of deoxynivalenol, while Leuconostoc mesenteroides removed about 82% of fumonisin B 1 , and Lact.…”