Biological Control of Molds and Mycotoxins in Foods

Bianchini, Andréia; Bullerman, Lloyd B.

doi:10.1021/bk-2009-1031.ch001

Cited by 21 publications

(20 citation statements)

References 67 publications

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“…The inhibitory effect in AFs production observed in the presence of LAB isolates also could be attributed to microbial mycotoxin degradation or to a sequestration effect caused by the LAB cells. Some of these antimicrobial LAB proprieties have been documented in the literature (Schnürer and Magnusson 2005;Bianchini and Bullerman 2009). …”

Section: Discussionmentioning

confidence: 99%

“…Lactic acid bacteria have been widely used in food and are 'generally regarded as safe' organisms (GRAS). They are used in commercial applications as biological control agents in processed foods to prevent fungal growth, to improve the shelf life of fermented products and to reduce potential health hazards associated with mycotoxins (Bianchini and Bullerman 2009). Several investigations indicate that LAB has beneficial health effects in humans and animals (Saxelin et al 2005;Perelmuter et al 2008;Oelschlaeger 2010).…”

Section: Introductionmentioning

confidence: 99%

“…isolated from several substrates as sourdoughs starters, bakery products and plant among others, and mycotoxigenic species belonging to Aspergillus, Penicillium and Fusarium genera (Batish et al 1989;Roy et al 1996;Gourama 1997;Corsetti et al 1998;Laitila et al 2002;Lavermicocca et al 2000Lavermicocca et al , 2003Magnusson et al 2003;Sjögren et al 2003;Schwenninger et al 2005;Bullerman et al 2006;Coda et al 2008;Hassan and Bullerman 2008;Gerez et al 2009). In the literature, Aspergillus section Flavi species growth has been described as controlled by other molds, yeasts and bacteria mainly Bacillus spp., propionic acid bacteria and LAB isolates (Wiseman and Marth 1981;Batish et al 1989;Karunaratne et al 1990;Gourama andBullerman 1995a, b, 1997;Schnürer and Magnusson 2005;Bianchini and Bullerman 2009). …”

Section: Introductionmentioning

confidence: 99%

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Effect of acid lactic bacteria isolated from faeces of healthy dogs on growth parameters and aflatoxin B1 production by Aspergillus species in vitro

et al. 2011

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The aim of the present study was to evaluate the inhibitory effect of Enterococcus faecium and Lactococcus lactis subsp. lactis isolated from faeces of healthy dogs on (i) lag phase, (ii) growth rate, and (iii) aflatoxin B1 production by Aspergillus section Flavi on in vitro assays. Thirteen lactic acid bacteria (LAB) isolates were used as antagonist microorganisms. Antagonistic activity was assayed against four potentially aflatoxigenic Aspergillus section Flavi isolates: A. flavus (AF210 and AF281), A. parasiticus (AP245) and A. parasiticus (NRRL 2999). In general, the longest lag phases of Aspergillus isolates were obtained with E. faecium GJ40. Respecting the growth rate, no significant reduction was found in this parameter in the interaction assays with A. flavus and antagonist isolates respecting the control. While in A. parasiticus a significant reduction in growth rate was only observed in the interaction among reference strain and E. faecium MF5 isolate (p < 0.05). In general, AFB1 production was reduced by most of the LAB isolates assayed, except for E. faecium GJ18, GJ20, MF3 and MF4. This study provides the first data about the antiaflatoxigenic activity of autochthonous LAB isolated from dog faeces.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of acid lactic bacteria isolated from faeces of healthy dogs on growth parameters and aflatoxin B1 production by Aspergillus species in vitro

et al. 2011

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“…Microbes have also been assessed as biocontrol agents for preventing aflatoxin contamination (Palumbo et al, 2008;Bianchini & Bullerman, 2010;Reddy et al, 2010). Atoxigenic strains of Aspergilli that can competitively exclude toxigenic strains from crops have been used for aflatoxin control (Brown et al, 1991;Cotty, 1994;Dorner & Cole, 2002), but some of atoxigenic strains produce a toxic metabolite, cyclopiazonic acid (Abbas et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Prevention of aflatoxin contamination by a soil bacterium of Stenotrophomonas sp. that produces aflatoxin production inhibitors

et al. 2013

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A soil bacterium, designated strain no. 27, was found to produce aflatoxin-production inhibitors. The strain was identified as a species of the genus Stenotrophomonas, and was found to be closely related to Stenotrophomonas rhizophila. Two diketopiperazines, cyclo(L-Ala-L-Pro) and cyclo(L-Val-L-Pro), were isolated from the bacterial culture filtrate as main active components. These compounds inhibited aflatoxin production of Aspergillus parasiticus and Aspergillus flavus in liquid medium at concentrations of several hundred mM without affecting fungal growth. Both inhibitors inhibited production of norsorolinic acid, a biosynthetic intermediate involved in an early step of the aflatoxin biosynthetic pathway, and reduced the mRNA level of aflR, which is a gene encoding a key regulatory protein necessary for the expression of aflatoxin-biosynthetic enzymes. These results indicated that the inhibitors targets are present in early regulatory steps leading to AflR expression. Co-culture of strain no. 27 with aflatoxigenic fungi in liquid medium effectively suppressed aflatoxin production of the fungus without affecting fungal growth. Furthermore, application of the bacterial cells to peanuts in laboratory experiments and at a farmer's warehouse in Thailand by dipping peanuts in the bacterial cell suspension strongly inhibited aflatoxin accumulation. The inhibitory effect was dependent on bacterial cell numbers. These results indicated that strain no. 27 may be a practically effective biocontrol agent for aflatoxin control.

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“…Biocontrol is receiving special attention since bacterial strains that possess antimicrobial activity against other bacteria and/or moulds can be found in nature. [20][21][22][23][24] Lactic acid bacteria (LAB) such as Lactobacillus spp., Leuconostoc spp., Streptocccus spp. and Enterococcus spp., among others, produce antimicrobial compounds that are important in the bio-preservation of foods and feeds.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory properties ofEnterococcusspp. isolated from faeces of healthy dogs

Juri

Muzzolon

Dalcero

et al. 2013

Journal of Environmental Science and Health, Part B

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The aim of the present study was to evaluate the inhibitory effect by the cross-streak method of nine Enterococcus faecium strains isolated from faeces of healthy dogs and their treated and non-treated cell-free supernatant (CFS) by the well-diffusion test on the growth of potentially pathogenic bacteria isolated from clinical cases and aflatoxigenic Aspergillus section Flavi and the consequent aflatoxin B1 (AFB1) production. Results obtained from the cross-strake assay showed that E. faecium MF1, GJ18 and GJ40 presented the major inhibitory activity against all pathogenic strains assayed; E. faecium GJ40 produced the larger inhibitory zones (26-27 mm). Well-diffusion test results showed that the majority of the enterococci strains CFS had antimicrobial activity against the pathogenic microorganisms, especially on Gram negative indicators. Cell-free supernatant of E. faecium GJ40 was the one that produced the largest inhibition zones (14 to 21 mm) in the majority of the indicator microorganisms assayed. All supernatants treated with 10 N NaOH (pH6) showed no inhibitory effect on the indicator strain assayed. With respect to fungal inhibition, any of the CFS assayed significantly inhibited the Aspergillus strains growth. But, in general, all CFS reduced AFB1 production from 8 to 87%. The results demonstrate that enterococci isolated from healthy dog feaces produce substances with the capacity to inhibit some potential pathogenic bacteria growth and the capacity of inhibiting or reducing the AFB1 production in vitro.

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Biological Control of Molds and Mycotoxins in Foods

Cited by 21 publications

References 67 publications

Effect of acid lactic bacteria isolated from faeces of healthy dogs on growth parameters and aflatoxin B1 production by Aspergillus species in vitro

Effect of acid lactic bacteria isolated from faeces of healthy dogs on growth parameters and aflatoxin B1 production by Aspergillus species in vitro

Prevention of aflatoxin contamination by a soil bacterium of Stenotrophomonas sp. that produces aflatoxin production inhibitors

Inhibitory properties ofEnterococcusspp. isolated from faeces of healthy dogs

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