Reducing effect of mangiferin on serum uric acid levels in mice

Niu, Yanfen; Lu, Wei; Gao, Lihui; Lin, Hui‐Yi; Liu, Xu; Li, Ling

doi:10.3109/13880209.2012.663763

Cited by 28 publications

(19 citation statements)

References 29 publications

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“…In the previous study, we reported that compound 1 at a dose of 1.5 mg/kg markedly decreased the serum uric acid levels in the hyperuricaemic mice induced by uricase inhibitor potassium oxonate in a manner that was similar to allopurinol treatment (Li & Chen 2010;Niu et al 2012), although the oral bioavailability of compound 1 was only 1.2% (Han et al 2010). This hinted that the observed hypouricaemic effect of compound 1 was related to its metabolites.…”

Section: Discussionmentioning

confidence: 90%

“…In the previous study, we reported that mangiferin had potential hypouricaemic effects. Given intragastrically at a 1.5-6.0 mg/kg dose, mangiferin significantly decreased the serum urate levels in hyperuricaemic mice induced by potassium oxonate (an uricase inhibitor), in a dose-dependent manner (Li & Chen 2010;Niu et al 2012). The potency of hypouricaemic action was similar to that of allopurinol.…”

Section: Introductionmentioning

confidence: 76%

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Hypouricaemic action of mangiferin results from metabolite norathyriol via inhibiting xanthine oxidase activity

Niu

Liu

et al. 2016

Pharmaceutical Biology

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Context Mangiferin has been reported to possess a potential hypouricaemic effect. However, the pharmacokinetic studies in rats showed that its oral bioavailability was only 1.2%, suggesting that mangiferin metabolites might exert the action. Objective The hypouricaemic effect and the xanthine oxidase inhibition of mangiferin and norathyriol, a mangiferin metabolite, were investigated. Inhibition of norathyriol analogues (compounds 3-9) toward xanthine oxidase was also evaluated. Materials and methods For a dose-dependent study, mangiferin (1.5-6.0 mg/kg) and norathyriol (0.92-3.7 mg/kg) were administered intragastrically to mice twice daily for five times. For a timecourse study, mice received mangiferin and norathyriol both at a single dose of 7.1 mmol/kg. In vitro, inhibition of test compounds (2.4-2.4 mM) against xanthine oxidase activity was evaluated by the spectrophotometrical method. The inhibition type was identified from Lineweaver-Burk plots. Results Norathyriol (0.92, 1.85 and 3.7 mg/kg) dose dependently decreased the serum urate levels by 27.0, 33.6 and 37.4%, respectively. The action was more potent than that of mangiferin at the low dose, but was equivalent at the higher doses. Additionally, the hypouricaemic action of them exhibited a time dependence. In vitro, norathyriol markedly inhibited the xanthine oxidase activities, with the IC 50 value of 44.6 mM, but mangiferin did not. The kinetic studies showed that norathyriol was an uncompetitive inhibitor by Lineweaver-Burk plots. The structure-activity relationships exhibited that three hydroxyl groups in norathyriol at the C-1, C-3 and C-6 positions were essential for maintaining xanthine oxidase inhibition. Discussion and conclusion Norathyriol was responsible for the hypouricaemic effect of mangiferin via inhibiting xanthine oxidase activity. ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 76%

Hypouricaemic action of mangiferin results from metabolite norathyriol via inhibiting xanthine oxidase activity

Niu

Liu

et al. 2016

Pharmaceutical Biology

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“…12) In the current study, it was demonstrated that mangiferin was able to significantly reduce the serum urate levels in uric acid-induced hyperuricemic mice, in a dose-and time-dependent manner, exhibiting a uricosuric effect. The uricosuric action was as potent as that of benzbromarone, although the onset of mangiferin was not as fast as that of benzbromarone with the onset time being less than 1 h after the administration.…”

Section: Discussionmentioning

confidence: 90%

“…1), 1,3,6,7-tetrahydroxyxanthone-C-2-β-Dglucoside, is a natural glucosyl xanthone from the leaves of Mangifera indica L. (Anacardiaceae), which is a tropical tree that was cultivated in many areas of the Old and the New World for its edible fruit. In previous study, we found that mangiferin had the potent hypouricemic effect associated with inhibiting the activity ofxanthine dehydrogenase/oxidase, at a dose of 1.5-6.0 mg/kg, as well as oral safety with a dose of 25.0 g/kg in mice, 12) exhibiting the potential to be developed as a new therapeutic agent for treatment of hyperuricemia and gout. However, the effects of mangiferin on renal excretion of urate and mechanisms of mangiferin-mediated hypouricemic action have not been completely elucidated.…”

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confidence: 99%

Mangiferin Inhibits Renal Urate Reabsorption by Modulating Urate Transporters in Experimental Hyperuricemia

Yang

Gao

Niu

et al. 2015

Biological & Pharmaceutical Bulletin

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Mangiferin, a natural glucosyl xanthone from the leaves of Mangifera indica L., was previously shown to exert potent hypouricemic effects associated with inhibition of the activity of xanthine dehydrogenase/ oxidase. The present study aimed to evaluate its uricosuric effect and possible molecular mechanisms underlying the renal urate transporters responsible for urate reabsorption in vivo. Mangiferin (1.5-24.0 mg/kg) was administered intragastrically to hyperuricemic mice and rats induced by the intraperitoneal injection of uric acid and potassium oxonate, respectively. The uricosuric effect was evaluated by determining the serum and urinary urate levels as well as fractional excretion of uric acid (FEUA). The mRNA and protein levels of renal urate-anion transporter 1 (URAT1), organic anion transporter 10 (OAT10), glucose transporter 9 (GLUT9), and PDZ domain-containing protein (PDZK1) were analyzed. The administration of mangiferin significantly decreased the serum urate levels in hyperuricemic mice in a dose-and time-dependent manner. In hyperuricemic rats, mangiferin also reduced the serum urate levels and increased the urinary urate levels and FEUA. These results indicate that mangiferin has uricosuric effects. Further examination showed that mangiferin markedly inhibited the mRNA and protein expression of renal URAT1, OAT10, and GLUT9 in hyperuricemic rats, but did not interfere with PDZK1 expression. Taken together, these findings suggest that mangiferin promotes urate excretion by the kidney, which may be related to the inhibition of urate reabsorption via downregulation of renal urate transporters.

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“…In addition to this, gouticin also contains Apium graveolens, Colchicum autumnale, Tribulus terrestris, and Withania somnifera along with Z. officinale Rosc. [17]. Our study showed that nearly 55% of inhibition from the flavonoid extract obtained from the rhizome of Z. officinale compared with allopurinol.…”

Section: Discussionmentioning

confidence: 84%

Evaluation of Xanthine Oxidase Inhibitory Activity by Flavonoids From Pongamia Pinnata Linn

Bambrana

Sheela

2017

Asian J Pharm Clin Res

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Objective: Flavonoids from the crude seeds extract of Pongamia pinnata L., dried fruit powder of Morinda citrifolia L., bark of Mangifera indica L., and rhizome of Zingiber officinale Rosc. were screened for xanthine oxidase (XO) inhibition at different concentration. The inhibitory potential of quercetin and allopurinol were used for the determination of 50% inhibitory concentration (IC 50 ) and K i values.Methods: Isolation of flavonoids from the plant extracts was processed by column chromatography and tested for XO inhibitory activity in the range of 6-800 µg/ml. Results:The results demonstrated that optimized flavonoids extract of P. pinnata L. exhibited promising XO inhibition. P. pinnata L., M. indica L., and Z. officinale Rosc. had IC 50 in the concentration of 8.74 mM, 1.09 mM, 5.4 mM and Ki 0.35 mM, 1.73 mM, 2.7 mM, respectively. Conclusion:The study showed that plant species under investigation exhibited XO inhibition by optimized flavonoid extract. P. pinnata L. indicated promising XO inhibition compared to other plant extracts. Flavonoids can be used as a potent inhibitor of XO an alternative to allopurinol.

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Reducing effect of mangiferin on serum uric acid levels in mice

Cited by 28 publications

References 29 publications

Hypouricaemic action of mangiferin results from metabolite norathyriol via inhibiting xanthine oxidase activity

Hypouricaemic action of mangiferin results from metabolite norathyriol via inhibiting xanthine oxidase activity

Mangiferin Inhibits Renal Urate Reabsorption by Modulating Urate Transporters in Experimental Hyperuricemia

Evaluation of Xanthine Oxidase Inhibitory Activity by Flavonoids From Pongamia Pinnata Linn

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