Reduced leaf peroxidase activity is associated with reduced lignin content in transgenic poplar.

Ipekci, Z.; Oğraş, Tijen Talas; Altlnkut, Ahu; Bajroviç, Kasım; Kazan, Kemal; Gözükırmızı, Nermin; Boydak, Melih; Tank, Turan; Akalp, Tahsin; Özden, Öznur; Çalıkoğlu, M. Erem; Tunçtaner, Korhan; Tulukçu, Mümtaz; Balkan, Hülya; Tanriyar, H.

doi:10.5511/plantbiotechnology.16.381

Cited by 24 publications

(8 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of calcium chloride (CaCl 2 ) sprays could increase the Ca concentrations in Anjou fruit and reduce the incidence of these disorders (Raese and Electronic supplementary material The online version of this article (doi:10.1007/s00344-014-9461-x) contains supplementary material, which is available to authorized users. others 1995, 1999Drake 1993, 1997;Richardson and Lombard 1979). In ripened fruit, CaCl 2 sprays before harvest resulted in increased fruit firmness, a lower ethylene production and respiration rate, slightly greener fruit, and better maintenance of acidity and extractable juices than in the unsprayed control treatment (Raese and others 1999).…”

Section: Introductionmentioning

confidence: 93%

Expression Analysis of Lignin-Associated Genes in Hard End Pear (Pyrus pyrifolia Whangkeumbae) and Its Response to Calcium Chloride Treatment Conditions

Zhang

et al. 2014

J Plant Growth Regul

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 93%

Expression Analysis of Lignin-Associated Genes in Hard End Pear (Pyrus pyrifolia Whangkeumbae) and Its Response to Calcium Chloride Treatment Conditions

Zhang

et al. 2014

J Plant Growth Regul

View full text Add to dashboard Cite

show abstract

“…CAld5H has not been studied in P. taeda cell cultures since they were derived from a gymnosperm, but one may extrapolate, in the absence of available data, that, like C4H and C3H in gymnosperms, CAld5H might also constitute a rate-limiting step in angiosperms. A similar role, as a rate-limiting step, has recently been proposed for the polymerization of monolignols catalyzed by basic pI class III plant peroxidases (Ipekci et al, 1999;Talas-Ogras et al, 2001;Blee et al, 2003). In this context, all the branching (also rate limiting) enzymes of the lignin biosynthetic pathway (C4H, C3H, and CAld5H) and the ligninassembling enzyme (peroxidase) are hemoproteins.…”

mentioning

confidence: 99%

Cloning and Molecular Characterization of the Basic Peroxidase Isoenzyme from Zinnia elegans, an Enzyme Involved in Lignin Biosynthesis

et al. 2005

View full text Add to dashboard Cite

The major basic peroxidase from Zinnia elegans (ZePrx) suspension cell cultures was purified and cloned, and its properties and organ expression were characterized. The ZePrx was composed of two isoforms with a M r (determined by matrix-assisted laser-desorption ionization time of flight) of 34,700 (ZePrx34.70) and a M r of 33,440 (ZePrx33.44). Both isoforms showed absorption maxima at 403 (Soret band), 500, and 640 nm, suggesting that both are high-spin ferric secretory class III peroxidases. M r differences between them were due to the glycan moieties, and were confirmed from the total similarity of the N-terminal sequences (LSTTFYDTT) and by the 99.9% similarity of the tryptic fragment fingerprints obtained by reverse-phase nano-liquid chromatography. Four full-length cDNAs coding for these peroxidases were cloned. They only differ in the 5#-untranslated region. These differences probably indicate different ways in mRNA transport, stability, and regulation. According to the k cat and apparent K m RH values shown by both peroxidases for the three monolignols, sinapyl alcohol was the best substrate, the endwise polymerization of sinapyl alcohol by both ZePrxs yielding highly polymerized lignins with polymerization degrees $87. Western blots using anti-ZePrx34.70 IgGs showed that ZePrx33.44 was expressed in tracheary elements, roots, and hypocotyls, while ZePrx34.70 was only expressed in roots and young hypocotyls. None of the ZePrx isoforms was significantly expressed in either leaves or cotyledons. A neighbor-joining tree constructed for the four full-length cDNAs suggests that the four putative paralogous genes encoding the four cDNAs result from duplication of a previously duplicated ancestral gene, as may be deduced from the conserved nature and conserved position of the introns.

show abstract

“…It plays a crucial role in reinforcing the plant cell wall structure. Biosynthesis of lignin in plant begins with the oxidative coupling of three monolignols as a building block of coniferyl, sinapyl, and p-coumaryl alcohols via phenylpropanoid pathway and the last step of lignification is the polymerization of cinnamyl alcohols by POD and using hydrogen peroxide as a substrate [24,25]. However, the expression of genes encoding plant lignification enzymes, including PAL, trans-cinnamate 4-hydroxylase, 4-coumarate CoA-ligase, ferulate 5-hydroxylase, COMT, and CCoAOMT, was repressed under flooding condition indicating that there was alternated lignin biosynthesis [26].…”

Section: Lignin Determinationmentioning

confidence: 99%

Capillary Water in Pericarp Enhances Hypoxic Condition during On-Tree Fruit Maturation That Induces Lignification and Triggers Translucent Flesh Disorder in Mangosteen (Garcinia mangostanaL.)

Noichinda

Bodhipadma

Kong-In

2017

Journal of Food Quality

View full text Add to dashboard Cite

Translucent flesh of mangosteen normally occurs during fruit ripening. Rainfall, after water stress, enhanced on-tree mature green fruit to develop translucent flesh disorder more frequently. Thus, this research pursued the effect of applied water on translucent flesh disorder development. The on-tree mature green stage fruits were selected and wrapped with 3 layers of fabric sheet. After that, water was continuously dropped (flow rate of 0.6 ml/min) on the wrapped sheet for 0, 1, and 2 days before picking. The results showed that duration time of water applying enhanced the increasing of water absorption significantly in peel. All of water-treated fruits ripened within 2-3 days after harvest and obviously had high lignin in secondary cell wall. It was hypothesized that lignification played an important role in hypoxia defense mechanism since the Na2CO3-SP fractionation extracted from alcohol insoluble residue (AIR) of translucent flesh aril was higher than those of normal aril. This Na2CO3-SP reinforced the strength of cell wall complexity as well as displaying the translucency character. Hence, we concluded that the capillary water (took place in intercellular air space of fruit pericarp) induced hypoxia tolerance mechanism that triggered translucent flesh disorder in mangosteen aril.

show abstract

Reduced leaf peroxidase activity is associated with reduced lignin content in transgenic poplar.

Cited by 24 publications

References 33 publications

Expression Analysis of Lignin-Associated Genes in Hard End Pear (Pyrus pyrifolia Whangkeumbae) and Its Response to Calcium Chloride Treatment Conditions

Expression Analysis of Lignin-Associated Genes in Hard End Pear (Pyrus pyrifolia Whangkeumbae) and Its Response to Calcium Chloride Treatment Conditions

Cloning and Molecular Characterization of the Basic Peroxidase Isoenzyme from Zinnia elegans, an Enzyme Involved in Lignin Biosynthesis

Capillary Water in Pericarp Enhances Hypoxic Condition during On-Tree Fruit Maturation That Induces Lignification and Triggers Translucent Flesh Disorder in Mangosteen (Garcinia mangostanaL.)

Contact Info

Product

Resources

About