Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples

Rocha, Giovanna S.; Silva, Martin; Cesarino, Ivana

doi:10.3390/ma13071751

Cited by 16 publications

(10 citation statements)

References 35 publications

(40 reference statements)

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“…Moreover, ΔE p of 237 mV for the GC/rGO-EDC-NHS/Ab electrode and 358 mV after the SARS-CoV-2 antigen is incubated are observed. This increase in the ΔE p was also observed in the antigen-antibody binding procedures of different types of electrochemical immunosensors [ 19 , 22 ]. In addition, Figure 2 C showed that the decrease of currents generated by the [Fe(CN) 6 ] 4− / 3− system observed in the cyclic voltammograms after SARS-CoV-2 antigen binding at the GC/rGO-EDC-NHS/Ab/BSA electrode has a clear correlation with the concentration of the antigen.…”

Section: Resultsmentioning

confidence: 55%

“…Our research group used the reduced graphene oxide (rGO) to immobilize enzymes for the preparation of enzymatic biosensors to monitor glucose during the second-generation ethanol production [ 16 ], to analyze neurotransmitters in urine and plasmatic serum samples [ 17 ] and to determine pesticides in food [ 18 ]. rGO was also employed as a platform in the development of an electrochemical biosensor for detection of staphylococcal enterotoxin A in milk samples [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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A Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosensor

et al. 2021

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Due to the SARS-CoV-2 pandemic, there has been an increase in the search for affordable healthcare devices for mass testing and rapid diagnosis. In this context, this work described a new methodology for SARS-CoV-2 detection based on an impedimetric immunosensor developed using the advantageous immobilization of antibodies in the reduced graphene oxide (rGO). The rGO was obtained by chemical synthesis from the commercial graphene oxide (GO), and the materials were morphologically, electrochemically and visually characterized. The cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques were used to evaluate the fabrication steps of the immunosensor. The electrochemical immunoassay was considered for SARS-CoV-2 spike protein RBD detection using a impedimetric immunosensor and redox couple ([(Fe(CN)6)]3−/4−) as a probe. The immunosensor was effectively developed and applied in the detection of SARS-CoV-2 spike protein RBD in saliva samples.

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Section: Resultsmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

A Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosensor

et al. 2021

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“…The research shows that antibodies and antigens have poor electrical conductivity, which could prevent the external redox mediator ([Fe(CN) 6 ] 3−/4− ) electrons’ transfer toward the electrode surface [ 20 ]. The EIS has been demonstrated to analyze the conductivity of the modified electrode.…”

Section: Resultsmentioning

confidence: 99%

“…Hence, the appropriate design and optimization of IDME is crucial for the detection performance. In addition, Au has been widely applied for IDME construction due to the simple fabrication and ease in forming self-assembled monolayers [ 20 ]. To pursue a higher sensitivity of a bioimpedance sensor, it is necessary to increase the amount of antibody as much as possible on the surface of an electrode.…”

Section: Introductionmentioning

confidence: 99%

An Innovative Nanobody-Based High-Biocompatibility Gold Interdigitated Microelectrode Electrochemical Bioimpedance Sensor for the Ultrasensitive Detection of Difenacoum in Human Serum

Guo

Wang

et al. 2021

Materials

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Difenacoum (DIF) is one of the most widely used anticoagulant rodenticides. However, accidental or intentional ingestion of DIF seriously threatens humans and other non-target species. Therefore, a rapid and sensitive detection method to quantify DIF is urgently needed. In this study, one anti-DIF nanobody (Nb) was assembled on the surface of a gold interdigitated microelectrode (IDME) using an Au–S bond to fabricate a bioimpedance sensor. To improve the immobilization amount of Nbs on the electrode, a polycrystalline gold IDME was prepared to provide a larger surface and better biocompatibility. Thus, a novel and ultrasensitive bioimpedance sensor based on electrochemical impedance spectroscopy (EIS) was designed for the determination of DIF, and it displayed good reproducibility and stability in human serum. The proposed bioimpedance sensor displayed a wide working range, between 0.1–1000 pg/mL, with a limit of detection (LOD) of 0.1 pg/mL of DIF. This method exhibited excellent performance, good sensitivity, and reproducibility and achieved the highest sensitivity of all currently existing methods used to quantify DIF. The highly sensitive DIF detection of this proposed bioimpedance sensor indicates its potential as an efficacious approach for DIF monitoring in human serum with high accuracy and precision.

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“…In addition, exploring methods for performance improving, application diversification and high reliability is also an adjoin research proposition, which lead most aspects of techniques to upgrade and even fuse. For these goals, many kinds of tentative studies have been done, such as the metal-pasted thread as the important conductive medium, the graphene-based conductive medium [12,13], and special synthetic textile materials as substrates. Certainly, the use of novel materials will push the fabrication techniques to make changes.…”

Section: Introductionmentioning

confidence: 99%

Wearable Textile UHF-RFID Sensors: A Systematic Review

2020

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Textile radio-frequency identification operating in ultra-high frequency (UHF-RFID) sensors based on different scenarios are becoming attractive with the forthcoming internet of things (IoT) era and aging society. Compared with conventional UHF-RFID sensors, textile UHF-RFID sensors offer the common textile features, light weight, washability and comfort. Due to the short time and low level of development, researches on the integration of textile UHF-RFID techniques and textile sensing techniques are not flourishing. This paper is motivated by this situation to identify the current research status. In this paper, we provide a systematic review of the fundamentals of textile UHF-RFID sensors techniques, materials, the brief history and the state-of-the-art of the scenario-based development through detailed summary and analysis on the achievements from the starting year of 2004 to the present time. Moreover, according to the analysis, we give a proposal of the future prospects in several aspects, including the new materials and manufacturing processes, machine learning technology, scenario-based applications and unavoidable reliability.

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Reduced Graphene Oxide-Based Impedimetric Immunosensor for Detection of Enterotoxin A in Milk Samples

Cited by 16 publications

References 35 publications

A Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosensor

A Novel Method for the Detection of SARS-CoV-2 Based on Graphene-Impedimetric Immunosensor

An Innovative Nanobody-Based High-Biocompatibility Gold Interdigitated Microelectrode Electrochemical Bioimpedance Sensor for the Ultrasensitive Detection of Difenacoum in Human Serum

Wearable Textile UHF-RFID Sensors: A Systematic Review

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