Reduced cell viabilityin vitro following exposure to a routine laboratory disinfectant

Boullier, Brian A.; Bosanquet, Andrew G.

doi:10.1007/bf00365090

Cytotechnology

1990

DOI: 10.1007/bf00365090

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Reduced cell viabilityin vitro following exposure to a routine laboratory disinfectant

Brian A. Boullier

Andrew G. Bosanquet

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1992

1994

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Airborne cytotoxicity in the DiSC assay caused by solutions of treosulfan but not busulphan

Bosanquet

Burlton

1994

Cytotechnology

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Treosulfan and busulphan are similar molecules, the former used in the treatment of ovarian cancer and the latter in chronic myelogenous leukaemia. We have used both in the differential staining cytotoxicity (DiSC) assay for in vitro drug sensitivity testing to aid in the choice of chemotherapy for individual patients. It was observed that occasionally the viability of control cells in one assay box was reduced compared with control cells in other boxes from the same assay. Treosulfan was suspected as the cause because cells throughout the microtitre box containing treosulfan had reduced viability in 28/62 (45%) experiments and in 9 of these, total kill of all cells in the box was observed. We tested the hypothesis that a metabolite of treosulfan might be the cause of this airborne cytotoxicity, and found that whilst 10 mg ml-1 of either methane sulphonic acid or tetrahydrofuran had no airborne cytotoxic effect, 1 mg ml-1 diepoxybutane killed over 95% of cells in all tubes in the same box. Treosulfan is another chemical (cf. azide, mafosfamide and possibly other cytotoxic agents) that can cause airborne cytotoxicity.

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Airborne cytotoxicity in the DiSC assay caused by solutions of treosulfan but not busulphan

Bosanquet

Burlton

1994

Cytotechnology

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Thein VitroRadiosensitivity of Lymphocytes from Chronic Lymphocytic Leukaemia Using the Differential Staining Cytotoxicity (DiSC) Assay. I—Investigation of the Method

Hinkley

Bosanquet

1992

International Journal of Radiation Biology

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A tumour sensitivity assay, the differential staining cytotoxicity (DiSC) assay, which has been shown to have potential in predicting tumour response to cytotoxic drugs, has been developed to investigate the radiosensitivity of peripheral blood lymphocytes isolated from patients with chronic lymphocytic leukaemia (CLL). The method involved irradiating isolated lymphocytes (0.64-20.48 Gy at 8 x 10(5) cells/ml) and incubating for 4 days. Subsequent radiation-induced cell kill was assessed by differential staining of dead and live cells and calculation of tumour cell viability. Factors affecting assay success rate and in vitro radioresponse were investigated. Results were shown to be reproducible both when repeat assays were performed on the same specimen on the same day, and on different specimens from the same patient after intervening periods of time. Greater than 1 day transit time (whole blood) or storage (separated cells in medium) was found to be detrimental to assay success, but pH of the medium (either pH 7.3 or 8) had little effect. An increased incubation period (from 4 to 7 days) slightly reduced cell survival, but the underlying radioresponsiveness of the cells did not change.

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Reduced cell viabilityin vitro following exposure to a routine laboratory disinfectant

Cited by 2 publications

References 2 publications

Airborne cytotoxicity in the DiSC assay caused by solutions of treosulfan but not busulphan

Airborne cytotoxicity in the DiSC assay caused by solutions of treosulfan but not busulphan

Thein VitroRadiosensitivity of Lymphocytes from Chronic Lymphocytic Leukaemia Using the Differential Staining Cytotoxicity (DiSC) Assay. I—Investigation of the Method

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