Reduced bioactive gibberellin content in rice seeds under low temperature leads to decreased sugar consumption and low seed germination rates

Wang, Yaliang; Cui, Yongtao; Guohui, Hu; Wang, Xiaodan; Chen, Huizhe; Shi, Qinghua; Xiang, Jing; Zhang, Yikai; Zhu, Dan; Zhang, Yuping

doi:10.1016/j.plaphy.2018.10.020

Cited by 64 publications

(36 citation statements)

References 33 publications

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“…Finally, the absorbance of 0.2 mL of the toluene phase (upper phase) was read at 520 nm using a spectrophotometer (PowerWave XS2). Soluble sugar concentration was measured following the methods used previously (Wang et al ., ) with minor modifications. The early seedling tissues (0.2 g) were ground with 1 mL of distilled water, heated at 100 °C for 10 min, cooled, centrifuged at 8000 g for 10 min at 25 °C and diluted to a volume of 10 mL with distilled water.…”

Section: Methodsmentioning

confidence: 99%

The lipid transfer protein OsLTPL159 is involved in cold tolerance at the early seedling stage in rice

Zhao

Wang

Qin

et al. 2019

Plant Biotechnology Journal

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Summary Nonspecific lipid transfer proteins (nsLTPs) play critical roles in plant development and response to abiotic stresses. Here, we found that a rice lipid transfer protein, OsLTPL159, was associated with cold tolerance at the early seedling stage. Overexpression of an OsLTPL159IL112 allele from the cold‐tolerant introgression line IL112 in either the japonica variety Zhonghua17 (ZH17) or the indica variety Teqing background dramatically enhanced cold tolerance. In addition, down‐regulation of the expression of OsLTPL159 in the japonica variety ZH17 by RNA interference (RNAi) significantly decreased cold tolerance. Further transcriptomic, physiological and histological analysis showed that the OsLTPL159IL112 allele likely enhanced the cold tolerance of rice at the early seedling stage by decreasing the toxic effect of reactive oxygen species, enhancing cellulose deposition in the cell wall and promoting osmolyte accumulation, thereby maintaining the integrity of the chloroplasts. Notably, overexpression of another allele, OsLTPL159GC2, from the recipient parent Guichao 2 (GC2), an indica variety, did not improve cold tolerance, indicating that the variations in the OsLTPL159 coding region of GC2 might disrupt its function for cold tolerance. Further sequence comparison found that all 22 japonica varieties surveyed had an OsLTPL159 haplotype identical to IL112 and were more cold‐tolerant than the surveyed indica varieties, implying that the variations in OsLTPL159 might be associated with differential cold tolerance of japonica and indica rice. Therefore, our findings suggest that the OsLTPL159 allele of japonica rice could be used to improve cold tolerance of indica rice through a molecular breeding strategy.

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Section: Methodsmentioning

confidence: 99%

The lipid transfer protein OsLTPL159 is involved in cold tolerance at the early seedling stage in rice

Zhao

Wang

Qin

et al. 2019

Plant Biotechnology Journal

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“…Additionally, grain number per panicle, panicle length, and grain weight were decline in response to cold climates 27 . Rice seed germination and some physiological parameters during seed germination are affected by low temperature 28–30 . Low temperature (19–20 °C) results in a reduction in photosynthetic parameters and affects antioxidant enzyme activity and membrane lipid peroxidation 31 .…”

Section: Introductionmentioning

confidence: 99%

Responses of plant growth, physiological, gas exchange parameters of super and non-super rice to rhizosphere temperature at the tillering stage

Jiang

Wang

et al. 2019

Sci Rep

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Temperature is one of the critical factors affecting rice growth and yield production. This study investigated the effects of rhizosphere temperature at the tillering stage on the growth, physiological parameters and gas exchange attributes of two rice cultivars, i.e., the super rice cultivar Yuxiangyouzhan and the non-super rice cultivar Xiangyaxiangzhan, under hydroponic conditions. Three rhizosphere temperature treatments, i.e., a low-rhizosphere-temperature treatment (LT, nutrient solution at 17.5 ± 1.5 °C), an ambient-temperature treatment (AT, nutrient solution at 27.5 ± 1.5 °C), and a high-rhizosphere-temperature treatment (HT, nutrient solution at 37.5 ± 1.5 °C), were applied in this study. The results showed significant differences in most of the rice growth and physiological and gas exchange parameters as a result of cultivar and rhizosphere temperature as well as their interaction. In addition, the marked reduction in total dry weight was positively correlated with a notable reduction in plant morphological parameters, such as the fresh and dry weight of the leaves and stem sheaths, and changes in gas exchange parameters. Moreover, antioxidant reactions were active in response to high and low rhizosphere temperatures, which varied in different plant tissues. These results suggested that the super and non-super rice were sensitive to high and low rhizosphere temperatures, respectively.

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“…physiological changes in the seeds, such as deactivation of gibberellin (GA), abscisic acid (ABA) synthesis, reduction in starch hydrolysis, and other processes that, in combination, contribute to reduction in germination (Wang et al, 2018). In germination of the species Paeonia ostii, it was observed that in addition to eff ects related to ABA induction and lower starch degradation, low temperature induced lower protein synthesis and transcription of genes involved in the germination process (Ren et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

GERMINATION AND POST-SEMINAL DEVELOPMENT OF Melaleuca alternifolia (MAIDEN & BETCHE) CHEEL

et al. 2020

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There is little information regarding the germination pattern, seed characterization, and seedling development of Melaleuca alternifolia. This study aimed to determine the ideal temperature for the germination of M. alternifolia seeds, as well as to characterize the seeds and the post-seminal development of the species. Three lots of M. alternifolia seeds were placed to germinate at 20, 25, 20-30, 20-35, 30, and 35 °C, with daily evaluations to determine germination rate, germination speed index (GSI), speed of germination (SG), and mean time for germination of 50% (t50) and 100% (t100) of seeds. The inner morphology of the seeds was evaluated through X-ray images and seedling morphology by photographs. Alternating temperatures (20-30 and 20-35 °C) led to germination rates higher than the other temperatures. The GSI had the lowest values at the constant temperature of 20 °C and the highest values at the constant 30 °C for all the lots. The SG was lowest at a temperature of 20 °C and the highest at the temperature of 35 °C. At alternating temperatures (20-30 and 20-35 °C), t50 was around 5 days and t100 reached 16 days. In conclusion, the alternating temperatures of 20-30 °C and 20-35 °C are recommended for germination of M. alternifolia, and final evaluation can be performed at 16 days after sowing. The inner structures of seeds could be visualized by X-ray analysis, and full seeds could be distinguished from empty seeds and impurities. Seeds exhibit epigeal germination and seedling development is stabilized after 30 days.

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Reduced bioactive gibberellin content in rice seeds under low temperature leads to decreased sugar consumption and low seed germination rates

Cited by 64 publications

References 33 publications

The lipid transfer protein OsLTPL159 is involved in cold tolerance at the early seedling stage in rice

The lipid transfer protein OsLTPL159 is involved in cold tolerance at the early seedling stage in rice

Responses of plant growth, physiological, gas exchange parameters of super and non-super rice to rhizosphere temperature at the tillering stage

GERMINATION AND POST-SEMINAL DEVELOPMENT OF Melaleuca alternifolia (MAIDEN & BETCHE) CHEEL

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