The lipid transfer protein Os<scp>LTPL</scp>159 is involved in cold tolerance at the early seedling stage in rice

Zhao, Jie; Wang, Shanshan; Qin, Jingjing; Sun, Chuanqing; Liu, Fengxia

doi:10.1111/pbi.13243

Cited by 60 publications

(45 citation statements)

References 84 publications

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“…“Pectinesterase inhibitor activity” (GO: 0046910) and some genes categorized under “hydrolase activity, acting on glycosyl bonds” (GO: 0016798) relates stiffening or loosening of cell walls (Hong et al., 2010; Sharma et al., 2013). “Lipid transport” (GO: 0006869) is also involved in stress adaptation (Guo et al., 2013; Zhao et al., 2020).…”

Section: Resultsmentioning

confidence: 99%

Overexpression of exogenous biuret hydrolase in rice plants confers tolerance to biuret toxicity

et al. 2020

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Urea, currently the most widely used nitrogen (N) fertilizer worldwide (IFASTAT, https://www.ifast at.org/datab ases), might contain biuret [(CONH 2) 2 NH], as a common impurity. Biuret is formed by the thermal condensation of urea. It has been known since the 1950s that excessive amounts of biuret in urea fertilizers cause injury in crops (Jones, 1954; Sanford et al., 1954). A wide range of crops can be potentially affected by biuret toxicity, which often manifests as leaf chlorosis and stunted growth, especially in the young seedling stage (Mikkelsen, 1990). Earlier studies indicated that biuret inhibited protein synthesis in Xanthium pensylvanicum leaves (Webster et al., 1957) and wheat (Triticum aestivum) germplasms (Ogata & Yamamoto, 1959). The protein content, however, did not so much decrease in biuret-injured orange (Citrus sinensis) leaves (Impey & Jones, 1960). It remains uncertain whether biuret has a direct effect on the protein synthetic machinery. Additionally, ultrastructural analyses showed that changes in chloroplast structure in biuret-injured leaves were similar to those in senescent leaves in grapefruit (Citrus paradise) and orange plants (Achor & Albrigo, 2005). Moreover biuret seems to remain unmetabolized in plants, because it was still detected in orange leaves, eight months after foliar spraying was performed (Impey & Jones, 1960). The exact mechanism underlying biuret toxicity in plants, however, is still far from being understood.

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Section: Resultsmentioning

confidence: 99%

Overexpression of exogenous biuret hydrolase in rice plants confers tolerance to biuret toxicity

et al. 2020

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“…Cold tolerance is a complex trait controlled by multiple genes ( Zhang et al, 2014 ), and the genes that have been isolated thus far can be divided into two different types, those that regulate gene expression during stress responses, including signaling components and transcription factors (TFs) ( Huang et al, 2012 ), and those that have specific functions and participate in systemic metabolism to defend against cold stress ( Huang et al, 2012 ). For example, OsLTPL159 enhances the cold tolerance of rice at the early seedling stage by decreasing the toxic effect of reactive oxygen species, enhancing cellulose deposition in the cell wall, and promoting osmolyte accumulation ( Zhao et al, 2020 ). Moreover, CTB4a , as a leucine-rich repeat receptor-like kinase, positively regulates the activity and content of ATP under cold stress by interacting with the β subunit of ATP synthase, thereby increasing pollen fertility ( Zhang et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of Candidate Genes Conferring Cold Tolerance to Rice (Oryza sativa L.) at the Bud-Bursting Stage Using Bulk Segregant Analysis Sequencing and Linkage Mapping

et al. 2021

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Low-temperature tolerance during the bud-bursting stage is an important characteristic of direct-seeded rice. The identification of cold-tolerance quantitative trait loci (QTL) in species that can stably tolerate cold environments is crucial for the molecular breeding of rice with such traits. In our study, high-throughput QTL-sequencing analyses were performed in a 460-individual F2:3 mapping population to identify the major QTL genomic regions governing cold tolerance at the bud-bursting (CTBB) stage in rice. A novel major QTL, qCTBB9, which controls seed survival rate (SR) under low-temperature conditions of 5°C/9 days, was mapped on the 5.40-Mb interval on chromosome 9. Twenty-six non-synonymous single-nucleotide polymorphism (nSNP) markers were designed for the qCTBB9 region based on re-sequencing data and local QTL mapping conducted using traditional linkage analysis. We mapped qCTBB9 to a 483.87-kb region containing 58 annotated genes, among which six predicted genes contained nine nSNP loci. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis revealed that only Os09g0444200 was strongly induced by cold stress. Haplotype analysis further confirmed that the SNP 1,654,225 bp in the Os09g0444200 coding region plays a key role in regulating the cold tolerance of rice. These results suggest that Os09g0444200 is a potential candidate for qCTBB9. Our results are of great significance to explore the genetic mechanism of rice CTBB and to improve the cold tolerance of rice varieties by marker-assisted selection.

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“…Plant nsLTP can respond to cold stress, such as by promoting the overexpression of AtLTP3 , which reduces electrolyte leakage induced by cold stress to improve soluble sugar accumulation and the survival rate of A . thaliana (Debono et al ., 2009), and inducing the expression of OsLTPL159 , which has been shown to regulate the activity of POD enzymes in rice to improve plant cold tolerance (Zhao et al ., 2020). However, the TIL‐mediated molecular regulatory mechanism of nsLTP has not yet been studied and the biological function of nsLTPs has not been reported in chrysanthemum.…”

Section: Introductionmentioning

confidence: 99%

Lysine crotonylation of DgTIL1 at K72 modulates cold tolerance by enhancing DgnsLTP stability in chrysanthemum

Huang

Liao

Yang

et al. 2021

Plant Biotechnology Journal

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Summary Lysine crotonylation of proteins is a recently identified post‐translational modification (PTM) in plants. However, the function of lysine‐crotonylated proteins in response to abiotic stress in plants has not been reported. In this study, we identified a temperature‐induced lipocalin‐1‐like gene (DgTIL1) from chrysanthemum and showed that it was notably induced in response to cold stress. Overexpression of DgTIL1 enhanced cold tolerance in transgenic chrysanthemum. Ubiquitin membrane yeast two‐hybrid (MYTH) system and bimolecular fluorescence complementation (BIFC) assays showed that DgTIL1 interacts with a nonspecific lipid transfer protein (DgnsLTP), which can promote peroxidase (POD) gene expression and POD activity to reduce the accumulation of reactive oxygen species (ROS) and improve resistance to cold stress in DgnsLTP transgenic chrysanthemum. In addition, we found that DgTIL1 was lysine crotonylated at K72 in response to low temperature in chrysanthemum. Moreover, lysine crotonylation of DgTIL1 prevented DgnsLTP protein degradation in tobacco and chrysanthemum. Inhibition of DgnsLTP degradation by lysine crotonylation of DgTIL1 further enhanced POD expression and POD activity, reduced the accumulation of ROS under cold stress in DgTIL1 transgenic chrysanthemum, thus promoting the cold resistance of chrysanthemum.

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The lipid transfer protein OsLTPL159 is involved in cold tolerance at the early seedling stage in rice

Cited by 60 publications

References 84 publications

Overexpression of exogenous biuret hydrolase in rice plants confers tolerance to biuret toxicity

Overexpression of exogenous biuret hydrolase in rice plants confers tolerance to biuret toxicity

Identification of Candidate Genes Conferring Cold Tolerance to Rice (Oryza sativa L.) at the Bud-Bursting Stage Using Bulk Segregant Analysis Sequencing and Linkage Mapping

Lysine crotonylation of DgTIL1 at K72 modulates cold tolerance by enhancing DgnsLTP stability in chrysanthemum

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