Lysine crotonylation of DgTIL1 at K72 modulates cold tolerance by enhancing DgnsLTP stability in chrysanthemum

Abstract

Summary Lysine crotonylation of proteins is a recently identified post‐translational modification (PTM) in plants. However, the function of lysine‐crotonylated proteins in response to abiotic stress in plants has not been reported. In this study, we identified a temperature‐induced lipocalin‐1‐like gene (DgTIL1) from chrysanthemum and showed that it was notably induced in response to cold stress. Overexpression of DgTIL1 enhanced cold tolerance in transgenic chrysanthemum. Ubiquitin membrane yeast two‐hybrid (MYTH) system and bimolecular fluorescence complementation (BIFC) assays showed that DgTIL1 interacts with a nonspecific lipid transfer protein (DgnsLTP), which can promote peroxidase (POD) gene expression and POD activity to reduce the accumulation of reactive oxygen species (ROS) and improve resistance to cold stress in DgnsLTP transgenic chrysanthemum. In addition, we found that DgTIL1 was lysine crotonylated at K72 in response to low temperature in chrysanthemum. Moreover, lysine crotonylation of DgTIL1 prevented DgnsLTP protein degradation in tobacco and chrysanthemum. Inhibition of DgnsLTP degradation by lysine crotonylation of DgTIL1 further enhanced POD expression and POD activity, reduced the accumulation of ROS under cold stress in DgTIL1 transgenic chrysanthemum, thus promoting the cold resistance of chrysanthemum.

DgbZIP3 interacts with DgbZIP2 to increase the expression ofDgPODfor cold stress tolerance in chrysanthemum

“…The constitutive expression of CdICE1 in chrysanthemum improved low-temperature tolerance [ 14 ]. Overexpression of DgTIL1 improved the tolerance of chrysanthemum to low-temperature stress, and the lysine crotonylation of DgTIL1 prevented the degradation of the non-specific lipid transfer protein DgnsLTP in chrysanthemum, thereby promoting the activity of peroxidase (POD) and reactive oxygen species (ROS) scavenging and therefore improving the tolerance of chrysanthemum to low-temperature stress [ 15 ]. DgMYB2 promotes increased glutathione peroxidase (GPX) activity by regulating the expression level of DgGPX1, reduces the accumulation of ROS in chrysanthemum, and improves the tolerance of chrysanthemum to low-temperature stress [ 16 ].…”

Secretory lipid transfer protein OsLTPL94 acts as a target of EAT1 and is required for rice pollen wall development

“…Because LTPs contain a hydrophobic cavity formed by eight conserved cysteine residues and can host different types of lipid molecules, they are also termed non‐specific LTPs (nsLTPs) (Buhot et al., 2004; Edqvist et al., 2018; Kader, 1996; Salminen et al., 2016). Plant nsLTPs are involved in key processes of plant biology, including seed development (Wang et al., 2015), wax assembly (Hollenbach et al., 1997), cell wall extension (Nieuwland et al., 2005), plant height (Deng et al., 2019), pathogen defense signaling (Deeken et al., 2016) and stress adaption (Guo, Yang, et al., 2013; Huang et al., 2020; Zhao, Wang, et al., 2020). Moreover, a growing body of research suggests that LTPs also have critical roles in pollen development.…”

Qualitative Proteome-Wide Analysis Reveals the Diverse Functions of Lysine Crotonylation in Dendrobium huoshanense

“…With a disturb to plant growth and gene potential, these stresses were treated as the most challengeable problem in agriculture, which can further entail a loss of grain yield at more than 50% ( Boyer, 1982 ; Parry and Hawkesford, 2012 ). It was surprising that crotonylated proteins had been proved to reduce the accumulation of reactive oxygen species (ROS) under low temperature stress and improve cold tolerance in Chrysanthemum ( Huang et al, 2021 ). Hsp70, a highly conserved protein, had been reported to play a vital role in maintaining cellular homeostasis and protecting living organism against stress, such as through viral suppression ( Vega et al, 2006 ).…”