Redox potential control and applications in microaerobic and anaerobic fermentations

Liu, Chen‐Guang; Xue, Chuang; Lin, Yen‐Han; Bai, Feng‐Wu

doi:10.1016/j.biotechadv.2012.11.005

Cited by 222 publications

(103 citation statements)

References 79 publications

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“…The regulatory effect of zinc on sugar transport and metabolism Compared to glucose, non-glucose sugars such as fructose and xylose were utilized slowly during ABE fermentations by C. acetobutylicum L7, however, significant improvements (Table 1) were achieved by micronutrient zinc while the ratios of butanol to ABE were relatively constant from these carbon sources (data not shown), indicating that these experimental results obtained were not ascribed to the redirection of carbon metabolic flux that was affected by intracellular redox balance (37). Furthermore, given the importance of the improved fructose/ xylose utilization in this study as well as glucose as previously described (21), micronutrient zinc played a significant role in the improved specific sugar consumption/growth/butanol production rates (Table 1).…”

Section: Resultscontrasting

confidence: 64%

Impact of zinc supplementation on the improved fructose/xylose utilization and butanol production during acetone–butanol–ethanol fermentation

Xue

Chen

et al. 2016

Journal of Bioscience and Bioengineering

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Section: Resultscontrasting

confidence: 64%

Impact of zinc supplementation on the improved fructose/xylose utilization and butanol production during acetone–butanol–ethanol fermentation

Xue

Chen

et al. 2016

Journal of Bioscience and Bioengineering

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“…Nutrient deficiency during fermentation and extracellular acidification due to acid production can affect intracellular pH (pH i ) (1)(2)(3). Characterizing the underlying mechanisms that cause and affect intracellular acidification and the molecular basis of pH homeostasis is important because such understanding also enables the optimization of industrial processes.…”

mentioning

confidence: 99%

Noninvasive High-Throughput Single-Cell Analysis of the Intracellular pH of Saccharomyces cerevisiae by Ratiometric Flow Cytometry

Valkonen

Mojžita

Penttilä

et al. 2013

Appl Environ Microbiol

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The ability of cells to maintain pH homeostasis in response to environmental changes has elicited interest in basic and applied research and has prompted the development of methods for intracellular pH measurements. Many traditional methods provide information at population level and thus the average values of the studied cell physiological phenomena, excluding the fact that cell cultures are very heterogeneous. Single-cell analysis, on the other hand, offers more detailed insight into population variability, thereby facilitating a considerably deeper understanding of cell physiology. Although microscopy methods can address this issue, they suffer from limitations in terms of the small number of individual cells that can be studied and complicated image processing. We developed a noninvasive high-throughput method that employs flow cytometry to analyze large populations of cells that express pHluorin, a genetically encoded ratiometric fluorescent probe that is sensitive to pH. The method described here enables measurement of the intracellular pH of single cells with high sensitivity and speed, which is a clear improvement compared to previously published methods that either require pretreatment of the cells, measure cell populations, or require complex data analysis. The ratios of fluorescence intensities, which correlate to the intracellular pH, are independent of the expression levels of the pH probe, making the use of transiently or extrachromosomally expressed probes possible. We conducted an experiment on the kinetics of the pH homeostasis of Saccharomyces cerevisiae cultures grown to a stationary phase after ethanol or glucose addition and after exposure to weak acid stress and glucose pulse. Minor populations with pH homeostasis behaving differently upon treatments were identified.

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“…It is reported that more reducing powers drive more metabolic flux toward more reducing products (Liu et al, 2013a). Mannitol, with an oxidation state of À1, generates 1 mol NADH more than glucose per mole consumed (Fig.…”

Section: Mannitol Utilization For Succinic Acid Production With Diffementioning

confidence: 98%

Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli

Bai

Zhou

Yang

et al. 2015

Bioresource Technology

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Redox potential control and applications in microaerobic and anaerobic fermentations

Cited by 222 publications

References 79 publications

Impact of zinc supplementation on the improved fructose/xylose utilization and butanol production during acetone–butanol–ethanol fermentation

Impact of zinc supplementation on the improved fructose/xylose utilization and butanol production during acetone–butanol–ethanol fermentation

Noninvasive High-Throughput Single-Cell Analysis of the Intracellular pH of Saccharomyces cerevisiae by Ratiometric Flow Cytometry

Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli

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