Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli

Bai, Bing; Zhou, Jiemin; Yang, Maohua; Liu, Yilan; Xu, Xiaohui; Xing, Jianmin

doi:10.1016/j.biortech.2015.02.081

Cited by 51 publications

(21 citation statements)

References 36 publications

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“…Also, several engineered E. coli strains were successfully applied for succinic acid production in two‐stage processes (see Table ) and further used to identify the optimal state of transition from the first culture to yield highly active and long‐time producing bacteria in the second process stage. For a two‐stage process with the succinic acid producer E. coli BS002 it was shown that cells from the early in contrast to cells from late exponential phase of the aerobic growth stage showed reduced glucose consumption and succinic acid formation in the anaerobic production stage . Wu et al.…”

Section: Zero‐growth Bioprocesses With Facultative Anaerobesmentioning

confidence: 99%

“…Also, several engineered E. coli strains were successfully applied for succinic acid production in two-stage processes (see Table 1) and further used to identify the optimal state of transition from the first culture to yield highly active and long-time producing bacteria in the second process stage. For a two-stage process with the succinic acid producer E. coli BS002 it was shown that cells from the early in contrast to cells from late exponential phase of the aerobic growth stage showed reduced glucose consumption and succinic acid formation in the anaerobic production stage [56]. Wu et al [42] conducted growth of E. coli NZN111 in the aerobic stage on various gluconeogenic substrates (pyruvate, glycerol, succinate, malate, α-ketoglutarate) and found that growth on malate led to a yield of 0.87 mol succinate per mol glucose (see Table 1), which is about seven times higher compared to conditions with glucose as growth substrate.…”

Section: Two-stage Processmentioning

confidence: 99%

“…Dual-phase processes were applied for succinic acid [9,12,16,41,59,60] and D,L-alanine [61] production with tailored E. coli strains, but also conducted with C. glutamicum strains engineered for isobutanol [10] or succinic acid [16] production. Most approaches performed the shift to anaerobiosis at a certain biomass concentration or point in process time but often without considering the cell's history or the physiological state of the culture [56,58,59,[61][62][63][64].…”

Section: Dual-phase Processmentioning

confidence: 99%

See 2 more Smart Citations

Zero‐growth bioprocesses: A challenge for microbial production strains and bioprocess engineering

Lange

Blombach

2016

Engineering in Life Sciences

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Microbial fermentation of renewable feedstocks is an established technology in industrial biotechnology. Besides strict aerobic or anaerobic modes of operation, novel innovative and industrially applicable fermentation processes were developed connecting the advantages of aerobic and anaerobic conditions in a combined production approach. As a consequence, rapid aerobic biomass formation to high cell densities and subsequent anaerobic high‐yield and zero‐growth production is realized. Following this strategy, bioprocesses operating with substantial overall yield and productivity can be obtained. Here, we summarize the current knowledge and achievements in such microbial zero‐growth production processes and pinpoint to challenges due to the complex adaptation of the cellular metabolism during the cell's passage from aerobiosis to anaerobiosis.

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Section: Zero‐growth Bioprocesses With Facultative Anaerobesmentioning

confidence: 99%

Section: Two-stage Processmentioning

confidence: 99%

Section: Dual-phase Processmentioning

confidence: 99%

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Zero‐growth bioprocesses: A challenge for microbial production strains and bioprocess engineering

Lange

Blombach

2016

Engineering in Life Sciences

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“…Macroalgae, also denoted seaweeds, are explored as a potential sustainable biomass resource for microbial conversion into ethanol and butanol (Hou et al, 2015;Hou et al, 2017) and other platform chemicals, like succinic acid and 2,3-butanediol (Mazumdar et al, 2013;Bai et al, 2015;Marquez et al, 2015;Milledge and Harvey, 2016). The global production of seaweed reached 30.4 million tons in 2015, and cultivated seaweed constitutes more than 95% of the total production, with China as the biggest producer (FAO, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Recombinant strains of the mesophilic bacteria Escherichia coli and Corynebacterium glutamicum are used for production of biofuels, chemicals and amino acids. E. coli can naturally assimilate both mannitol and glucose and has been engineered to produce 17.4 g/l of succinic acid from a hydrolyzate made from brown seaweed Saccharina japonica (Bai et al, 2015). Recently, the lysine producing strain C. glutamicum LYS-12 was engineered to assimilate mannitol and efficiently produce 2 g/l L-lysine from this substrate in shake flask experiments (Hoffmann et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Production of Value-Added Chemicals by Bacillus methanolicus Strains Cultivated on Mannitol and Extracts of Seaweed Saccharina latissima at 50°C

et al. 2020

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The facultative methylotroph Bacillus methanolicus MGA3 has previously been genetically engineered to overproduce the amino acids L-lysine and L-glutamate and their derivatives cadaverine and γ-aminobutyric acid (GABA) from methanol at 50 • C. We here explored the potential of utilizing the sugar alcohol mannitol and seaweed extract (SWE) containing mannitol, as alternative feedstocks for production of chemicals by fermentation using B. methanolicus. Extracts of the brown algae Saccharina latissima harvested in the Trondheim Fjord in Norway were prepared and found to contain 12-13 g/l of mannitol, with conductivities corresponding to a salt content of ∼2% NaCl. Initially, 12 B. methanolicus wild type strains were tested for tolerance to various SWE concentrations, and some strains including MGA3 could grow on 50% SWE medium. Non-methylotrophic and methylotrophic growth of B. methanolicus rely on differences in regulation of metabolic pathways, and we compared production titers of GABA and cadaverine under such growth conditions. Shake flask experiments showed that recombinant MGA3 strains could produce similar and higher titers of cadaverine during growth on 50% SWE and mannitol, compared to on methanol. GABA production levels under these conditions were however low compared to growth on methanol. We present the first fed-batch mannitol fermentation of B. methanolicus and production of 6.3 g/l cadaverine. Finally, we constructed a recombinant MGA3 strain synthesizing the C30 terpenoids 4,4 -diaponeurosporene and 4,4 -diapolycopene, experimentally confirming that B. methanolicus has a functional methylerythritol phosphate (MEP) pathway. Together, our results contribute to extending the range of both the feedstocks for growth and products that can be synthesized by B. methanolicus.

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Probiotic Fermentation of Kelp Enzymatic Hydrolysate Promoted its Anti‐Aging Activity in D‐Galactose‐Induced Aging Mice by Modulating Gut Microbiota

Xiong

Jiang

et al. 2023

Molecular Nutrition Food Res

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Scope: To investigate anti-aging effects of probiotic-fermented kelp enzymatic hydrolysate culture (KMF), probiotic-fermented kelp enzymatic hydrolysate supernatant (KMFS), and probiotic-fermented kelp enzymatic hydrolysate bacteria suspension (KMFP) in D-galactose-induced aging mice. Methods and results: The study uses a probiotic-mixture of Lactobacillus reuteri, Pediococcus pentosaceus, and Lactobacillus acidophilus strains for kelp fermentation. KMF, KMFS, and KMFP prevent D-galactose-induced elevation of malondialdehyde levels in serum and brain tissue of aging mice, and they increase superoxide dismutase and catalase levels and total antioxidant capacity. Furthermore, they improve the cell structure of mouse brain, liver, and intestinal tissue. Compared with the model control group, the KMF, KMFS, and KMFP treatments regulate mRNA and protein levels of genes associated with aging, the concentrations of acetic acid, propionic acid, and butyric acid in the three treatment groups are more than 1.4-, 1.3-, and 1.2-fold increased, respectively. Furthermore, the treatments affect the gut microbiota community structures. Conclusions: These results suggest that KMF, KMFS, and KMFP can modulate gut microbiota imbalances and positively affect aging-related genes to achieve anti-aging effects.

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Efficient production of succinic acid from macroalgae hydrolysate by metabolically engineered Escherichia coli

Cited by 51 publications

References 36 publications

Zero‐growth bioprocesses: A challenge for microbial production strains and bioprocess engineering

Zero‐growth bioprocesses: A challenge for microbial production strains and bioprocess engineering

Production of Value-Added Chemicals by Bacillus methanolicus Strains Cultivated on Mannitol and Extracts of Seaweed Saccharina latissima at 50°C

Probiotic Fermentation of Kelp Enzymatic Hydrolysate Promoted its Anti‐Aging Activity in D‐Galactose‐Induced Aging Mice by Modulating Gut Microbiota

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