Redox potential as a key parameter for monitoring and optimization of xylose fermentation with yeast Spathaspora passalidarum under limited-oxygen conditions

Bonan, C.I.D.G.; Biazi, Luiz Eduardo; Dionísio, S.R.; Soares, Lauren B.; Tramontina, Robson; Sousa, Amanda Silva de; Filho, Carlos Alberto de Oliveira; Costa, Aline Carvalho da; Ienczak, Jaciane Lutz

doi:10.1007/s00449-020-02344-2

Cited by 29 publications

(14 citation statements)

References 23 publications

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“…Biazi et al (2020) worked with a 1.4 L bioreactor and 900 mL of working volume using 30 g/L glucose, 75 g/L xylose, and 20 g/L of initial cell concentration at 30 °C, 48 h, 200 rpm, and a k L a of 8.0 h –1 . On the other hand, Bonan et al (2020) used 27.7 g/L of glucose and 66.3 g/L of xylose in a 3L bioreactor containing 1.4L of working volume, 17.2 g/L of initial cell concentration, at 30 °C, 48 h, 200 rpm, and a k L a of 4.9 h –1 . Both authors were able to obtain ethanol concentrations of 37.0 ± 0.09 g/L and 28.6 ± 0.4 g/L, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…The fermentation times were different in the works, but none of the studies reached high values of Y P/S and Q P . Biazi et al (2020) 58 and Bonan et al (2020) 81 worked with hemicellulosic hydrolysates obtained from acid pretreatment and S. passalidarum. Biazi et al (2020) 58 worked with a 1.4 L bioreactor and 900 mL of working volume using 30 g/L glucose, 75 g/L xylose, and 20 g/L of initial cell concentration at 30 °C, 48 h, 200 rpm, and a k L a of 8.0 h −1 .…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The high ethanol productivity obtained in this work may be related to the absence of inhibitors, the headspace volume of the flask, and the stirring rate, which led to good aeration rates. Bonan et al (2020) 81 studied the effect of oxygen availability on the fermentation by S. passalidarum and concluded that an adequate volumetric oxygen transfer coefficient combined with high cell density resulted in an increase in the Qp and a decrease in the time for sugar exhaustion.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Effect of Protic Ionic Liquids in Sugar Cane Bagasse Pretreatment for Lignin Valorization and Ethanol Production

Rabelo

et al. 2021

ACS Sustainable Chem. Eng.

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Ionic liquids have been called designer solvents because it is possible to fine-tune their properties from a huge range of possible combinations of cations and anions. In this work, we evaluate the potential of protic ionic liquids (PILs) in the fractionation of sugar cane bagasse in a biorefinery context. The PILs were designed to selectively solubilize lignin and leave a polysaccharides solid fraction. The carbohydrate fraction was further hydrolyzed to obtain pentose and hexose sugars, which were fermented using a pentose-consuming yeast. The results have shown that, differently from pretreatments that solubilize hemicelluloses, pentoses could be fermented together with hexoses without a prior detoxification step with high yield and productivity. The lignin recovered was characterized by 2D HSQC NMR, 31 P NMR, and GPC, showing that the design of the PIL influences lignin characteristics, but pretreatment temperature and time do not. The lignins obtained are sulfur-free, and the richness of their functional groups makes them attractive for conversion into a diversity of value-added products, which can make a biorefinery based on the proposed process viable.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

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Effect of Protic Ionic Liquids in Sugar Cane Bagasse Pretreatment for Lignin Valorization and Ethanol Production

Rabelo

et al. 2021

ACS Sustainable Chem. Eng.

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“… 196 Bonan et al (2020) attained highest ethanol titers of 28.6 g L −1 with 0.31 g g −1 yield and 1.12 g L −1 h −1 productivity with Spathaspora passalidarum at K La (h −1 ) of 45. 54 Hence, an ideal process engineering aspect would be to determine the optimal K La value which maximizes the xylose flux towards the desired product with minimal or no secretion of xylitol, eventually benefiting the cell growth and product formation. Alternative approaches such as overexpression of NOX, modification of the cofactor specificity of XR, or expression of NADH specific XR over NADPH-dependent XR can alleviate the problem of redox imbalance and replenish the flux towards central carbon metabolism.…”

Section: Challenges For Xylose Based Bioproductionmentioning

confidence: 99%

Valorisation of xylose to renewable fuels and chemicals, an essential step in augmenting the commercial viability of lignocellulosic biorefineries

Narisetty

Cox

Bommareddy

et al. 2022

Sustainable Energy Fuels

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“…Microorganisms that have the Crabtree effect produce ethanol when sugar concentrations are high, even in aerobic conditions. S. passalidarum is a Crabtree negative yeast, and cell production increases with higher aeration, and the choice to produce ethanol or cells depends on the concentration of O2 available to the cells [35,38]. In the case of the SBF with immobilized cells used in this work (shaker), the oxygen transfer was probable impaired due to the bioreactor and support choice, making it difficult to consume xylose and, consequently, to produce ethanol.…”

Section: Sbf With Immobilized and Free S Passalidarum Cellsmentioning

confidence: 99%

Immobilization of Spathaspora passalidarum NRRL Y-27907 in Calcium Alginate Aiming the Production of Second-Generation Ethanol

et al. 2021

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The immobilization of S. passalidarum in calcium alginate beads for second-generation ethanol production (E2G) was evaluated in a medium that simulated a hemicellulosic hydrolysate of sugarcane bagasse pretreated with diluted sulfuric acid in terms of sugars composition. Three sets of sequential batch fermentations (SBF) were carried out with free cells, or immobilized cells in high (HSC) and moderate (MSC) initial sugar concentration (120 and 70 g/L, respectively). SBF were characterized by five consecutive batches performed in shaker, at 30 °C and 110 rpm. Better results were observed for the SBF with immobilized cells in MSC medium when compared to HSC (Y'P/S of 0.27 ± 0.02 and 0.19 ± 0.03 g/g, respectively), in the second batch cycle. The value for YP/S in MSC was similar to the obtained with free cells (0.30 ± 0.02 to 0.33 ± 0.02 g/g). However, QP was lower for MSC, reaching 0.81 ± 0.04 g/L.h in in the second batch, while for free cells the QP varied from 1.06 ± 0.02 to 1.16 ± 0.22 g/L.h. A technique for determining the concentration of immobilized cells in the alginate beads was applied, which made it possible to determine the specific rates for the SBF performed. According to the results obtained, it was possible to demonstrate that S. passalidarum can be immobilized in calcium alginate and reuse through SBF, with performance similar to free cells, which can be a good strategy for fermentation of hemicellulosic hydrolysates.

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Redox potential as a key parameter for monitoring and optimization of xylose fermentation with yeast Spathaspora passalidarum under limited-oxygen conditions

Cited by 29 publications

References 23 publications

Effect of Protic Ionic Liquids in Sugar Cane Bagasse Pretreatment for Lignin Valorization and Ethanol Production

Effect of Protic Ionic Liquids in Sugar Cane Bagasse Pretreatment for Lignin Valorization and Ethanol Production

Valorisation of xylose to renewable fuels and chemicals, an essential step in augmenting the commercial viability of lignocellulosic biorefineries

Immobilization of Spathaspora passalidarum NRRL Y-27907 in Calcium Alginate Aiming the Production of Second-Generation Ethanol

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