Redox Homeostasis and Regulation in Pluripotent Stem Cells: Uniqueness or Versatility?

Ivanova, Julia; Lyublinskaya, O. G.

doi:10.3390/ijms222010946

Cited by 9 publications

(8 citation statements)

References 142 publications

(214 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is noteworthy that stem cells and cancer cells share strong similarities in the metabolic characteristics of glucose utilization, which may be attributed to the rapid proliferation, while anaerobic oxidation provides ATP and necessary metabolic intermediates for the biosynthesis demand [ 34 ]. In addition, PSCs show a plastic preferenec for anaerobic oxidation and conservative redox homeostasis, which may realize the control of ROS production and minimize the negative impact of oxidative damage via the maintenance of immature, low-quality mitochondria [ 11 ]. OXPHOS is inclined to establish a link with stem cell exhaustion caused by induced lineage commitment, while anaerobic oxidation has far-reaching significance for maintaining stem cell potential [ 12 , 13 ].…”

Section: Discussionmentioning

confidence: 99%

“…OXPHOS is inclined to establish a link with stem cell exhaustion caused by induced lineage commitment, while anaerobic oxidation has far-reaching significance for maintaining stem cell potential [ 12 , 13 ]. Metabolic reprogramming towards anaerobic oxidation would occur in induced pluripotent stem cells (iPSCs) from reprogrammed somatic cells, which can prove the substantial relevance of anaerobic oxidation to the pluripotency of stem cells [ 11 , 35 ]. We had assumed that the force-loading would increase aerobic oxidation since mechanical stimulation may cause oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

“…The physiological behavior of pluripotency stem cells (PSCs) under mechanical force is affected by various factors, such as gene and protein expression, signal pathway regulation, and metabolic key enzymes and processes [ 4 , 7 , 10 ]. Considerable studies have clarified that metabolic factors, especially the preference for glucose oxidation mode, have a crucial impact on the determination of stem cell fate, prevention of cellular stress, and maintenance of pluripotency [ 11 , 12 ]. Compared with the classic regulation of aerobic and anaerobic oxidation based on oxygen supply, the oxidative homeostasis of PSCs with high plasticity tends to anaerobic oxidation to adapt to stem cell maintenance and commitment [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mechanical force induces mitophagy-mediated anaerobic oxidation in periodontal ligament stem cells

Zhang

Cui

et al. 2023

Cell Mol Biol Lett

View full text Add to dashboard Cite

Background The preference for glucose oxidative mode has crucial impacts on various physiological activities, including determining stem cell fate. External mechanical factors can play a decisive role in regulating critical metabolic enzymes and pathways of stem cells. Periodontal ligament stem cells (PDLSCs) are momentous effector cells that transform mechanical force into biological signals during the reconstruction of alveolar bone. However, mechanical stimuli-induced alteration of oxidative characteristics in PDLSCs and the underlying mechanisms have not been fully elucidated. Methods Herein, we examined the expression of LDH and COX4 by qRT-PCR, western blot, immunohistochemistry and immunofluorescence. We detected metabolites of lactic acid and reactive oxygen species for functional tests. We used tetramethylrhodamine methyl ester (TMRM) staining and a transmission electron microscope to clarify the mitochondrial status. After using western blot and immunofluorescence to clarify the change of DRP1, we further examined MFF, PINK1, and PARKIN by western blot. We used cyclosporin A (CsA) to confirm the regulation of mitophagy and ceased the stretching as a rescue experiment. Results Herein, we ascertained that mechanical force could increase the level of LDH and decrease the expression of COX4 in PDLSCs. Simultaneously, the yield of reactive oxygen species (ROS) in PDLSC reduced after stretching, while lactate acid augmented significantly. Furthermore, mitochondrial function in PDLSCs was negatively affected by impaired mitochondrial membrane potential (MMP) under mechanical force, and the augment of mitochondrial fission further induced PRKN-dependent mitophagy, which was confirmed by the rescue experiments via blocking mitophagy. As a reversible physiological stimulation, the anaerobic preference of PDLSCs altered by mechanical force could restore after the cessation of force stimulation. Conclusions Altogether, our study demonstrates that PDLSCs under mechanical force preferred anaerobic oxidation induced by the affected mitochondrial dynamics, especially mitophagy. Our findings support an association between mechanical stimulation and the oxidative profile of stem cells, which may shed light on the mechanical guidance of stem cell maintenance and commitment, and lay a molecular foundation for periodontal tissue regeneration. Graphical Abstract

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mechanical force induces mitophagy-mediated anaerobic oxidation in periodontal ligament stem cells

Zhang

Cui

et al. 2023

Cell Mol Biol Lett

View full text Add to dashboard Cite

show abstract

“…ROS signaling plays a role in the immune system response, for example where neutrophils release superoxide to control bacterial infections 69 . Careful fine-tuning of ROS levels is also required in stem cells, where differences in concentration can directly impact cell fate decisions such as self renewal, stemness, differentiation, and senescence 70 . ROS is equally critical during aging.…”

Section: Discussionmentioning

confidence: 99%

Reactive Oxygen Species Signaling Differentially Controls Wound Healing and Regeneration

Huizen

Hack

Greene

et al. 2022

Preprint

View full text Add to dashboard Cite

Reactive oxygen species (ROS), such as hydrogen peroxide, are conserved and critical components of both wound healing and regeneration. Even though millions are affected each year by poor wound healing and an inability to restore functional tissue, how the same ROS-mediated signaling regulates these two different processes is not fully understood. Here, we investigate the role(s) of ROS during planarian wound healing and regeneration. We show ROS accumulate after injury and are required for wound closure (by promoting cytoskeletal movements) and regrowth (by promoting blastema formation). We found that different threshold levels of ROS regulate separate downstream targets to control wound healing (jun-1) versus regeneration (hsp70). By only manipulating ROS levels, we were able to control which injury-induced program was initiated: failure to close (chronic wound), healing only (no blastema), or full regeneration. Our results demonstrate that healing versus regenerative outcomes are based on differential ROS-mediated gene expression soon after injury. This study highlights ROS signaling as a potential therapeutic means to control wound repair mechanisms in multiple contexts. Therefore, investigating the mechanisms by which ROS control different tissue repair processes will be necessary not only for regenerative medicine but to improve clinical outcomes for chronic wounds and fibrosis.

show abstract

“…Metabolic pathways of polyploid cells also demonstrate manifestations of stemness [ 11 , 71 , 72 ]. Similarly to pluripotent stem cells, polyploid cells can simultaneously derive energy from pathways that are incompatible with differentiated cells, promoting adaptation to stress associated with energy deprivation [ 49 , 72 , 176 , 177 , 178 , 179 , 180 , 181 , 182 ]. Recent data obtained from polyploid cells from normal tissues of animals and plants, various tumors, and yeasts indicate that these cells possess energetically flexible metabolism combining glycolysis glutaminolysis and oxidative phosphorylation [ 43 , 57 , 72 , 183 , 184 , 185 ].…”

Section: Common Biological Effects Of Overexpressed Myc and Polyploid...mentioning

confidence: 99%

Polyploidy and Myc Proto-Oncogenes Promote Stress Adaptation via Epigenetic Plasticity and Gene Regulatory Network Rewiring

Anatskaya

Vinogradov

2022

IJMS

View full text Add to dashboard Cite

Polyploid cells demonstrate biological plasticity and stress adaptation in evolution; development; and pathologies, including cardiovascular diseases, neurodegeneration, and cancer. The nature of ploidy-related advantages is still not completely understood. Here, we summarize the literature on molecular mechanisms underlying ploidy-related adaptive features. Polyploidy can regulate gene expression via chromatin opening, reawakening ancient evolutionary programs of embryonality. Chromatin opening switches on genes with bivalent chromatin domains that promote adaptation via rapid induction in response to signals of stress or morphogenesis. Therefore, stress-associated polyploidy can activate Myc proto-oncogenes, which further promote chromatin opening. Moreover, Myc proto-oncogenes can trigger polyploidization de novo and accelerate genome accumulation in already polyploid cells. As a result of these cooperative effects, polyploidy can increase the ability of cells to search for adaptive states of cellular programs through gene regulatory network rewiring. This ability is manifested in epigenetic plasticity associated with traits of stemness, unicellularity, flexible energy metabolism, and a complex system of DNA damage protection, combining primitive error-prone unicellular repair pathways, advanced error-free multicellular repair pathways, and DNA damage-buffering ability. These three features can be considered important components of the increased adaptability of polyploid cells. The evidence presented here contribute to the understanding of the nature of stress resistance associated with ploidy and may be useful in the development of new methods for the prevention and treatment of cardiovascular and oncological diseases.

show abstract

Redox Homeostasis and Regulation in Pluripotent Stem Cells: Uniqueness or Versatility?

Cited by 9 publications

References 142 publications

Mechanical force induces mitophagy-mediated anaerobic oxidation in periodontal ligament stem cells

Mechanical force induces mitophagy-mediated anaerobic oxidation in periodontal ligament stem cells

Reactive Oxygen Species Signaling Differentially Controls Wound Healing and Regeneration

Polyploidy and Myc Proto-Oncogenes Promote Stress Adaptation via Epigenetic Plasticity and Gene Regulatory Network Rewiring

Contact Info

Product

Resources

About